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Axiooberver z1 inverted microscope

Manufactured by Zeiss
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The AxioOberver Z1 is an inverted microscope manufactured by Zeiss. It is designed for a wide range of applications, including cell biology, developmental biology, and other life science research. The microscope features a stable and ergonomic design, and includes advanced optics and illumination systems to provide high-quality imaging capabilities.

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Lab products found in correlation

Amca conjugated donkey anti goat igg h l, by Jackson ImmunoResearch (2 mentions) Irf4 antibody, by Santa Cruz Biotechnology (2 mentions) Biotinylated anti mouse cd138 clone 281 2, by BD (2 mentions) Bcl6 antibody, by Santa Cruz Biotechnology (2 mentions) Gam igd fc 7s, by Cedarlane (2 mentions) Fitc conjugated anti igd clone 11 26c 2a, by BD (2 mentions) Biotin conjugated anti cd45.1 clone a20, by BioLegend (2 mentions) Biotin conjugated anti dcir2 clone 33d1, by BioLegend (2 mentions) Biotin conjugated anti cd25 clone pc61, by BD (2 mentions) Fitc conjugated anti hrp, by Jackson ImmunoResearch (2 mentions) Ap conjugated anti fitc, by Jackson ImmunoResearch (2 mentions) Ap conjugated sa, by Jackson ImmunoResearch (2 mentions) Amca conjugated donkey anti goat igg, by Jackson ImmunoResearch (2 mentions) Alexa 488 conjugated donkey anti rabbit igg, by Thermo Fisher Scientific (2 mentions) Alexa 647 conjugated streptavidin, by Thermo Fisher Scientific (2 mentions) Tsa biotin system, by PerkinElmer (2 mentions) Vibratome, by Leica (1 mentions) 7mp two photon microscope, by Zeiss (1 mentions) Pkh26, by Merck Group (1 mentions) Zen 2012, by Zeiss (1 mentions)

Spelling variants of this product

Inverted microscope (490 citations) Z1 inverted microscope (28 citations) AxioOberver (4 citations) AxioOberver microscope (3 citations)

9 protocols using axiooberver z1 inverted microscope

1

Immunohistochemical Staining and Multiphoton Imaging of Murine Spleen

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Cryosections of 7 μm were fixed and stained immunohistochemically as previously described (Allen et al., 2004 (link)) with the following antibodies: PE-conjugated anti-IgD (11–26c.2a; Biolegend), biotin-conjugated anti-GL7 (GL7, Biolegend), and FITC-conjugated 33D1 antibody (33D1, Biolegend). Images were captured with a Zeiss AxioOberver Z1 inverted microscope. For multiphoton imaging, CD11cYFP mice were injected with PKH26 (Sigma-Aldrich)-labeled Cd47−/− RBCs 20 mins before the spleen was harvested and sectioned longitudinally with a vibratome (Leica). The cut spleen was perfused with RPMI1640 medium (Life Technologies) plus 10mM HEPES and 95% CO2 5% O2. The cut spleen was imaged with a 7MP two-photon microscope (Carl Zeiss) equipped with a Chameleon laser (Coherent). Excitation wavelengths were 940nm for YFP and 800nm for PKH26. The z-stack was 21μm thick with 3μm intervals. The image was acquired with ZEN2012 (Carl Zeiss) and the three dimensional movie was made with Imaris 7.4.2 x64 (Bitplane).
Yi T., Li J., Chen H., Wu J., An J., Xu Y., Hu Y., Lowell C.A, & Cyster J.G. (2015). Splenic dendritic cells survey red blood cells for missing self-CD47 to trigger adaptive immune responses. Immunity, 43(4), 764-775.
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2

Multicolor Immunofluorescence Staining Protocol

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Cryosections of 10 µm were fixed by acetone for 10 min, dried for 1 h, and stained with goat anti-IgD, FITC-conjugated anti-Marco (Bio-Rad), FITC-conjugated anti-CD169 (BioLegend), biotin-conjugated 33D1 (BioLegend), streptavidin-AF647 (Thermo Fisher Scientific), and AMCA-conjugated donkey anti-goat IgG (H+L; Jackson Immunoresearch). Primary antibody incubation was performed overnight at 4°C, followed by secondary staining for 3 h at room temperature. For staining of 33D1 (anti-DCIR2), the tyramide amplification kit was used (TSA Biotin System) according to the manual. Images were captured with a Zeiss AxioOberver Z1 inverted microscope and stitched by Zen software.
Liu D., Wu J., An J, & Cyster J.G. (2020). Requirements for cDC2 positioning in blood-exposed regions of the neonatal and adult spleen. The Journal of Experimental Medicine, 217(11), e20192300.
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3

Immunophenotyping of Mesenteric Lymph Nodes

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Mesenteric lymph nodes were fixed in 4% paraformaldehyde in PBS for 4 hours on ice and incubated in 30% sucrose overnight, followed by embedding in tissue freezing medium (OCT). Cryosections of 10 μm were stained with goat anti-IgD, rabbit anti-Tmem119, APC-conjugated anti-CD21/35, biotin-conjugated EpCAM, streptavidin-PE (Biolegend) and AMCA-conjugated donkey anti-goat IgG (H+L) (Jackson Immunoresearch). Images were captured with a Zeiss AxioOberver Z1 inverted microscope.
Duan L., Liu D., Chen H., Mintz M.A., Chou M.Y., Kotov D.I., Xu Y., An J., Laidlaw B.J, & Cyster J.G. (2021). Follicular dendritic cells restrict interleukin 4 availability in germinal centers and foster memory B cell generation. Immunity, 54(10), 2256-2272.e6.
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4

Immunophenotyping of Tumor Cryosections

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Cryosections 7 μm in thickness from mLN and spleen were cut and prepared as described3 . Tumor immunopheotyping was performed using goat polyclonal IRF4 antibody (Santa Cruz, sc-6059) or biotinylated anti-mouse CD138 (clone 281-2; BD Biosciences). For Bcl-6 staining, cryosections were fixed with 4% PFA for 10 minutes and stained with rabbit polyclonal Bcl6 antibody (Santa Cruz, sc-368). Images were captured with a Zeiss AxioOberver Z1 inverted microscope.
Muppidi J.R., Schmitz R., Green J.A., Xiao W., Larsen A.B., Braun S.E., An J., Xu Y., Rosenwald A., Ott G., Gascoyne R.D., Rimsza L.M., Campo E., Jaffe E.S., Delabie J., Smeland E.B., Braziel R.M., Tubbs R.R., Cook J.R., Weisenburger D.D., Chan W.C., Vaidehi N., Staudt L.M, & Cyster J.G. (2014). Loss of signaling via Gα13 in germinal center B cell-derived lymphoma. Nature, 516(7530), 254-258.
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5

Immunohistochemical Staining of Cryosections

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Cryosections of 7 μm were fixed and stained immunohistochemically as described17 (link),31 (link) with: FITC-conjugated anti-IgD (clone 11-26c.2a, BD Biosciences), biotin-conjugated anti-CD45.1 (clone A20, Biolegend, San Diego, CA, USA), biotin-conjugated anti-DCIR2 (clone 33D1, Biolegend) or biotin-conjugated anti-CD25 (clone PC61.5, BD Biosciences) followed by HRP-conjugated anti-FITC, AP-conjugated anti-FITC, and/or AP-conjugated SA (Jackson Immunoresearch, Newmarket, Suffolk, UK). For staining of DCIR2 and CD25, a tyramide amplification kit was used (TSA Biotin System; Perkin Elmer; Waltham, MA, USA). For immunofluorescence, staining was performed with biotin conjugated anti-CD45.1, rabbit anti-GFP (Molecular Probes, Eugene, OR, USA), and goat anti-mouse IgD (GAM/IGD(FC)/7S, Cedarlane Laboratories), followed by AMCA-conjugated donkey anti-goat IgG (Jackson Immunoresearch), Alexa 488-conjugated donkey anti-rabbit IgG and Alexa 647-conjugated streptavidin (Invitrogen). Images were captured with a Zeiss AxioOberver Z1 inverted microscope.
Li J., Lu E., Yi T, & Cyster J.G. (2016). EBI2 augments Tfh cell fate by promoting interaction with IL2-quenching dendritic cells. Nature, 533(7601), 110-114.
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6

Immunophenotyping of Tumor Cryosections

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Cryosections 7 μm in thickness from mLN and spleen were cut and prepared as described3 . Tumor immunopheotyping was performed using goat polyclonal IRF4 antibody (Santa Cruz, sc-6059) or biotinylated anti-mouse CD138 (clone 281-2; BD Biosciences). For Bcl-6 staining, cryosections were fixed with 4% PFA for 10 minutes and stained with rabbit polyclonal Bcl6 antibody (Santa Cruz, sc-368). Images were captured with a Zeiss AxioOberver Z1 inverted microscope.
Muppidi J.R., Schmitz R., Green J.A., Xiao W., Larsen A.B., Braun S.E., An J., Xu Y., Rosenwald A., Ott G., Gascoyne R.D., Rimsza L.M., Campo E., Jaffe E.S., Delabie J., Smeland E.B., Braziel R.M., Tubbs R.R., Cook J.R., Weisenburger D.D., Chan W.C., Vaidehi N., Staudt L.M, & Cyster J.G. (2014). Loss of signaling via Gα13 in germinal center B cell-derived lymphoma. Nature, 516(7530), 254-258.
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7

Multicolor Immunohistochemistry of Spleen

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Spleen tissue cryosections (10 μm) were fixed in acetone for 10 min, dried for 1 h and subjected to staining with goat anti-IgD, AF647-conjugated anti-IgM (RMM-1, 406526; 1:100), APC-conjugated anti-IgMa (MA-69, 408613; 1:100) and PE-conjugated anti-IgMb (AF6-78, 406208; 1:100) from Biolegend and AMCA-conjugated donkey anti-goat IgG (H + L; 705-155-147, 0.5 mg ml−1; 1:200) from Jackson Immunoresearch. Images were captured using a Zeiss AxioOberver Z1 inverted microscope and stitched together using ZEN 2 (blue edition).
Liu D., Winer B.Y., Chou M.Y., Tam H., Xu Y., An J., Gardner J.M, & Cyster J.G. (2023). Dynamic encounters with red blood cells trigger splenic marginal zone B cell retention and function. Nature Immunology, 25(1), 142-154.
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8

Immunohistochemical Staining of Cryosections

Cited 1 time
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Cryosections of 7 μm were fixed and stained immunohistochemically as described17 (link),31 (link) with: FITC-conjugated anti-IgD (clone 11-26c.2a, BD Biosciences), biotin-conjugated anti-CD45.1 (clone A20, Biolegend, San Diego, CA, USA), biotin-conjugated anti-DCIR2 (clone 33D1, Biolegend) or biotin-conjugated anti-CD25 (clone PC61.5, BD Biosciences) followed by HRP-conjugated anti-FITC, AP-conjugated anti-FITC, and/or AP-conjugated SA (Jackson Immunoresearch, Newmarket, Suffolk, UK). For staining of DCIR2 and CD25, a tyramide amplification kit was used (TSA Biotin System; Perkin Elmer; Waltham, MA, USA). For immunofluorescence, staining was performed with biotin conjugated anti-CD45.1, rabbit anti-GFP (Molecular Probes, Eugene, OR, USA), and goat anti-mouse IgD (GAM/IGD(FC)/7S, Cedarlane Laboratories), followed by AMCA-conjugated donkey anti-goat IgG (Jackson Immunoresearch), Alexa 488-conjugated donkey anti-rabbit IgG and Alexa 647-conjugated streptavidin (Invitrogen). Images were captured with a Zeiss AxioOberver Z1 inverted microscope.
Li J., Lu E., Yi T, & Cyster J.G. (2016). EBI2 augments Tfh cell fate by promoting interaction with IL2-quenching dendritic cells. Nature, 533(7601), 110-114.
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9

Immunohistochemistry of Paraformaldehyde-Fixed Tissue

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From paraformaldehyde-fixed tissue, 7 μm sections were prepared, as previously described (Gray et al., 2012 (link)). In some cases, sections were fixed by acetone (Gray et al., 2012 (link)). Sections were stained with the flowing antibodies: anti-Lyve1-A647, anti-CD3ε-bio (clone 145-2C11), Goat anti-CCL20 (AF760), Polyclonal Rabbit anti-GFP (Thermo Fisher Scientific), anti-B220-A647 (RA3-6B2), anti-ICAM1-bio (3E2), anti-IL7Rα-A647 (A7R34), rat anti-scart2, Hamster anti-TCRγδ (GL3), Donkey anti-Goat-biotin Jackson immunoresearch), Goat anti-Armenian Hamster-bio (Jackson immunoresearch), Donkey anti-Rat-bio (Jackson immunoresearch), Anti-biotin-A488 (Jackson immunoresearch), Anti-biotin-Cy3 (Jackson immunoresearch). Images were captured with a Zeiss AxioOberver Z1 inverted microscope.
Zhang Y., Roth T.L., Gray E.E., Chen H., Rodda L.B., Liang Y., Ventura P., Villeda S., Crocker P.R, & Cyster J.G. (2016). Migratory and adhesive cues controlling innate-like lymphocyte surveillance of the pathogen-exposed surface of the lymph node. eLife, 5, e18156.
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