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H3 trimethyl lys 27 antibody

Manufactured by Merck Group
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The H3 trimethyl Lys 27 antibody is a laboratory research tool used for the detection and quantification of histone H3 proteins with trimethylation at lysine 27. This modification is associated with epigenetic regulation of gene expression. The antibody can be used in various applications, such as Western blotting, immunoprecipitation, and chromatin immunoprecipitation, to study the role of this histone mark in cellular processes.

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Lab products found in correlation

Ez chip kit, by Merck Group (13 mentions) Sybr green mix, by Takara Bio (8 mentions) Ezh2 antibody, by Abcam (6 mentions) Ezh2 and suz12 antibodies, by Abcam (3 mentions) Histone h3, by Merck Group (3 mentions) Acetyl histone h3 lys27, by Merck Group (3 mentions) Ezh2 and suz12, by Abcam (2 mentions) Simple chip enzymatic chromatin ip kit, by Merck Group (1 mentions) Ezh2 antibody, by Merck Group (1 mentions) Sybr green master kit, by Roche (1 mentions) Ez magna chip a g one day chromatin immunoprecipitation kit, by Merck Group (1 mentions) Beacon designer 7, by Premier Biosoft (1 mentions) Suv39h1, by Abcam (1 mentions) E2f1 antibody, by Cell Signaling Technology (1 mentions)

17 protocols using h3 trimethyl lys 27 antibody

1

ChIP Assays for Epigenetic Modifications

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The ChIP assays were performed according to the Protocol for the fast chromatin immunoprecipitation (ChIP) method [42 (link)]. EZH2 antibody was purchased from Abcam. H3 trimethyl Lys 27 antibody was from Millipore. Gene specific primers for LIMK2b are listed in Additional file 5: Table S5. Results were normalized using the internal control IgG. Precipitated chromatin DNA was recovered and analyzed by qPCR.
Niu Y., Ma F., Huang W., Fang S., Li M., Wei T, & Guo L. (2017). Long non-coding RNA TUG1 is involved in cell growth and chemoresistance of small cell lung cancer by regulating LIMK2b via EZH2. Molecular Cancer, 16, 5.
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2

ChIP-qPCR Analysis of EZH2 and SUZ12

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The ChIP assays were performed using the EZ-CHIP KIT according to the manufacturer's instructions (Millipore, Billerica, MA, USA). EZH2 and SUZ12 antibodies were obtained from Abcam (Hercules, CA, USA). H3 trimethyl Lys 27 antibody was purchased from Millipore. Quantification of immunoprecipitated DNA was performed using qPCR with SYBR Green Mix (Takara). The ChIP data were calculated as a percentage relative to the input DNA using the equation 2[Input Ct−Target Ct] × 0.1 × 100.
Su J., Zhang E., Han L., Yin D., Liu Z., He X., Zhang Y., Lin F., Lin Q., Mao P., Mao W, & Shen D. (2017). Long noncoding RNA BLACAT1 indicates a poor prognosis of colorectal cancer and affects cell proliferation by epigenetically silencing of p15. Cell Death & Disease, 8(3), e2665-.
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3

Chromatin Immunoprecipitation (ChIP) Assay

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ChIP assays were conducted using the SimpleChIP®
Plus Enzymatic Chromatin IP Kit, according to the
manufacturer’s instructions (CST, USA). H3 trimethyl Lys
27 antibody was obtained from Millipore (USA). EZH2
(5246) antibody was obtained from CST. Quantification of
immunoprecipitated DNA was performed by quantitative
PCR (qPCR). ChIP data were calculated as a percentage
relative to the input DNA.
Zeng S.G., Xie J.H., Zeng Q.Y., Dai S.H., Wang Y., Wan X.M, & Liu J.C. (2021). lncRNA PVT1 Promotes Metastasis of Non-Small Cell Lung Cancer Through EZH2-Mediated Activation of Hippo/NOTCH1 Signaling Pathways. Cell Journal (Yakhteh), 23(1), 21-31.
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4

ChIP Assay Using EZ-CHIP Kit

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ChIP assays were performed using EZ-CHIP KIT according to the manufacturer’s instructions (Millipore, USA). EZH2 was obtained from Abcam. H3 trimethyl Lys 27 antibody, Histone H3, and Acetyl-Histone H3 Lys27 were from Millipore. The ChIP primer sequences are listed in Table S2. Quantification of immunoprecipitated DNA was performed using qPCR. ChIP data were calculated as a percentage relative to the input DNA by the following equation: 2[Input Ct − Target Ct] × 100 (%).
He X., Wang J., Chen J., Han L., Lu X., Miao D., Yin D., Geng Q, & Zhang E. (2019). lncRNA UCA1 Predicts a Poor Prognosis and Regulates Cell Proliferation and Migration by Repressing p21 and SPRY1 Expression in GC. Molecular Therapy. Nucleic Acids, 18, 605-616.
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5

ChIP-qPCR Assay for Histone Modifications

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Chromatin immunoprecipitation assay (ChIP) assays were performed using an EZ-CHIP KIT (Millipore). EZH2 antibody was obtained from Abcam. H3 trimethyl Lys 27 antibody was from Millipore. The ChIP primer sequences are listed in Supplementary Table 1. Quantification of immunoprecipitated DNA was performed using qPCR with SYBR Green Mix (TaKaRa). ChIP data were calculated as a percentage relative to the input DNA by the equation of 2(Input Ct - Target Ct) × 0.1 × 100.
Chen W.M., Chen W.D., Jiang X.M., Jia X.F., Wang H.M., Zhang Q.J., Shu Y.Q, & Zhao H.B. (2017). HOX transcript antisense intergenic RNA represses E-cadherin expression by binding to EZH2 in gastric cancer. World Journal of Gastroenterology, 23(33), 6100-6110.
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6

Chromatin Immunoprecipitation Protocol

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ChIP assays were performed using EZ-CHIP KIT according to the manufacturer, s instruction (Millipore, USA). EZH2 and SUZ12 antibodies were obtained from Abcam. E2F1 was from Cell Signaling Technology. H3 trimethyl Lys 27 antibody was from Millipore. The ChIP primer sequences were listed in Supplementary Table S1. Quantification of immunoprecipitated DNA was performed using qPCR with SYBR Green Mix (Takara). ChIP data was calculated as a percentage relative to the input DNA by the equation 2[Input Ct- Target Ct] × 0.1×100.
Zhang E.B., Kong R., Yin D.D., You L.H., Sun M., Han L., Xu T.P., Xia R., Yang J.S., De W, & Chen J.F. (2014). Long noncoding RNA ANRIL indicates a poor prognosis of gastric cancer and promotes tumor growth by epigenetically silencing of miR-99a/miR-449a. Oncotarget, 5(8), 2276-2292.
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7

Chromatin Immunoprecipitation (ChIP) Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
ChIP assays were performed using an EZ-CHIP KIT according to the manufacturer's instructions (Millipore, USA). EZH2 antibody was obtained from Abcam. H3 trimethyl Lys 27 antibody was from Millipore. The ChIP primer sequences are listed in Supplementary Table S1. Quantification of immunoprecipitated DNA was performed using qPCR with SYBR Green Mix (Takara). ChIP data is presented as a percentage relative to the input DNA using the equation 2[Input Ct- Target Ct] ×0.1 × 100 [19 (link)].
Chen W.M., Huang M.D., Sun D.P., Kong R., Xu T.P., Xia R., Zhang E.B, & Shu Y.Q. (2016). Long intergenic non-coding RNA 00152 promotes tumor cell cycle progression by binding to EZH2 and repressing p15 and p21 in gastric cancer. Oncotarget, 7(9), 9773-9787.
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8

ChIP Assay with EZH2 and H3K27me3

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ChIP assays were performed using EZ-CHIP KIT according to the manufacturer’s instruction (Millipore, USA). EZH2 antibody was obtained from Abcam. H3 trimethyl Lys 27 antibody was from Millipore. The ChIP primer sequences were listed in Additional file 4: Table S1. Quantification of immunoprecipitated DNA was performed using qPCR with SYBR Green Mix (Takara). ChIP data was calculated as a percentage relative to the input DNA by the equation 2[Input Ct − Target Ct] × 0.1 × 100.
Kong R., Zhang E.B., Yin D.D., You L.H., Xu T.P., Chen W.M., Xia R., Wan L., Sun M., Wang Z.X., De W, & Zhang Z.H. (2015). Long noncoding RNA PVT1 indicates a poor prognosis of gastric cancer and promotes cell proliferation through epigenetically regulating p15 and p16. Molecular Cancer, 14, 82.
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9

ChIP Assay for Epigenetic Regulation

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Simple ChIP Enzymatic Chromatin IP Kit (Millipore, USA) was used for ChIP assays according to the manufacturer's protocol. Cells were cross-linked with formaldehyde and collected in lysis buffer. Cell lysates were then sonicated to generate chromatin fragments of 200–300 bp and immunoprecipitated with EZH2 and H3K27me3-specific antibodies (CST) or IgG as control. Immunoprecipitated DNA was amplified by PCR using primers, which are listed in Supplementary Table 1. EZH2 antibodies were obtained from Abcam. The H3 trimethyl Lys 27 antibody was purchased from Millipore. Precipitated chromatin DNA was recovered and analyzed by qRT-PCR.
Sun Y., Jin S.D., Zhu Q., Han L., Feng J., Lu X.Y., Wang W., Wang F, & Guo R.H. (2017). Long non-coding RNA LUCAT1 is associated with poor prognosis in human non-small cell lung cancer and regulates cell proliferation via epigenetically repressing p21 and p57 expression. Oncotarget, 8(17), 28297-28311.
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10

ChIP Analysis of Histone Modifications

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The EZ-CHIP KIT was utilized to execute ChIP assays as stated by the instructions of the manufacturer (Millipore). The EZH2 antibody and H3 trimethyl Lys 27 antibody were acquired from Millipore and Abcam, respectively. qPCR was utilized to determine quantification of immunoprecipitated DNA, and the ChIP data was measured in relation to the input DNA: 2[Input Ct-Target Ct] ×0.1×100.
Zhu L., Zhang C., Xue J., He X., Yin D., Zhu Q., Shu Y, & De W. (2021). EZH2-mediated epigenetic suppression of lncRNA PCAT18 predicts a poor prognosis and regulates the expression of p16 by interacting with miR-570a-3p in gastric cancer. Journal of Cancer, 12(23), 7069-7078.
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