Plasma levels of total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL-c), and low-density lipoprotein (LDL-c) were measured at the Department of Medical Biochemistry, Oslo University Hospital. TC, TG, and HDL-c were directly measured using an Integra 800 instrument from Roche Diagnostics, according to standard methods. LDL-c was calculated by Friedewald formula at the Department of Medical Biochemistry, Oslo University Hospital. This method changed during the study to direct measurement of LDL-c using an Integra 800 instrument from Roche Diagnostics.
Integra 800
Manufactured by Roche
Sourced in Switzerland, United States, Germany
The Integra 800 is a versatile laboratory instrument designed for a range of analytical tasks. It features a high-performance liquid chromatography (HPLC) system and is capable of performing automated sample preparation, separation, and detection. The Integra 800 is a reliable and efficient tool for various analytical applications in research and clinical settings.
Automatically generated - may contain errors
Lab products found in correlation
Cobas integra 800, by Roche (2 mentions)
Eia kit, by R&D Systems (1 mentions)
Onetouch ultra, by Johnson & Johnson (1 mentions)
Radioimmunoassay kit, by DiaSorin (1 mentions)
Centaur xp, by Siemens (1 mentions)
Combi 514, by Hanil (1 mentions)
Tempus spin rna isolation kit, by Thermo Fisher Scientific (1 mentions)
Tina quant hemoglobin a1c gen 2, by Roche (1 mentions)
Elisa plate reader, by Agilent Technologies (1 mentions)
Procartaplex kit, by Thermo Fisher Scientific (1 mentions)
30 protocols using integra 800
1
Lipid Profile Measurement Protocols
2
Creatinine-based Kidney Function Assessment
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Serum creatinine was assessed using the Roche Integra 800 colorimetric assay (Roche Diagnostics Ltd., Rotkreuz, ZG, Switzerland), calibrated to the standards set by the National Institute of Standards and Technology (NIST). Estimated GFR (eGFR; in mL/min/1.73 m2) was calculated from serum creatinine using the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation [28 (link)]. A mid-stream urine sample was also collected using 50 mL specimen containers and assessed using the Roche Integra 800 colorimetric assay (Roche Diagnostics Ltd., Rotkreuz, ZG, Switzerland) to determine urinary albumin-creatinine ratio (UACR in mg/mmol). DKD was defined as eGFR <60 mL/min/1.73 m2 (N = 81) [29 (link)], and/or UACR >3.39 mg/mmol (equivalent to 30 mg/g) (N = 122) in this study. The CKD-EPI formula has been validated extensively in Asian populations with accuracy of estimating CKD similar to that reported in Caucasian studies [30 (link),31 (link)].
3
Pediatric Blood Sample Collection and Analysis
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Blood tests were performed at Princess Margaret Hospital for Children located in Perth (Western Australia). Each child supplied one blood sample upon recruitment. Following an overnight fast, blood was drawn for subsequent measurement of glucose, insulin and HbA1c levels. Blood glucose was determined using a spectrophotometric (colorimetric) method (Vitros Glu, Ortho-Clinical Diagnostics, NY, USA), plasma insulin by a chemiluminescent immunoassay (Immulite 2000 Diamond Diagnostics, MA, USA) [19 (link)] and HbA1c on a Roche Integra 800 (Roche Diagnostics, Mannheim, Germany). As per Figure 1 , there was some data missing for these measures, with these participants excluded.
4
Lipid Profile Measurement Protocol
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The lipid profile parameters: total cholesterol, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and triglycerides (TGs), were measured in the biochemistry laboratory at SMC, using Clinical Analyzer ROCHE COBAS INTEGRA 800 (Rotkreuz, Switzerland).
5
Clinical Biomarkers in Cardiometabolic Health
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BMI was calculated as the individual's body weight (in kilograms) divided by the square of their height (in meters). Blood sampling was performed between 8 am and 2 pm (in patients) and between 8 am and 5 pm (in healthy controls). The analysis of clinical chemistry parameters was performed at the Department of Clinical Chemistry, Oslo University Hospital, Oslo, Norway, on an Integra 800 from Roche Diagnostics (Basel, Switzerland), by using standard methods.
For the immunological analysis, blood was drawn into EDTA-containing vials, and plasma was extracted and stored at -80 °C. Plasma levels of hs-CRP, gp130, and sTNF-R1
were measured via enzyme immunoassay (EIA) kits obtained from R&D systems (Minneapolis MN, USA). Intra-and interassay coefficients of variance were less than 10%.
For the immunological analysis, blood was drawn into EDTA-containing vials, and plasma was extracted and stored at -80 °C. Plasma levels of hs-CRP, gp130, and sTNF-R1
were measured via enzyme immunoassay (EIA) kits obtained from R&D systems (Minneapolis MN, USA). Intra-and interassay coefficients of variance were less than 10%.
6
Lipid and Glucose Metabolism Assay
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Serum total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and glucose concentrations were measured by enzymatic assay using an automatic biochemical analyzer (ROCHE COBAS INTEGRA 800, Basel, Switzerland) from the fasting mice at the time-points dictated.
Three days before euthanasia, intraperitoneal Glucose Tolerance Test (ipGTT) was performed on 6 hrs fasted mice. The mice (n = 6 each group) were intraperitoneally injected with glucose (2 g/kg bodyweight, 20% glucose solution). Blood samples were respectively obtained from tail vein, at 0, 10, 30, 60, 90 and 120 min. after the glucose load, and blood glucose concentration was measured using a One Touch Ultra glucometer (Johnson&Johnson New Jersey, USA). The area under the curve (AUC) was then determined using the linear method of the trapezoid rule 21 (link),22 (link).
Three days before euthanasia, intraperitoneal Glucose Tolerance Test (ipGTT) was performed on 6 hrs fasted mice. The mice (n = 6 each group) were intraperitoneally injected with glucose (2 g/kg bodyweight, 20% glucose solution). Blood samples were respectively obtained from tail vein, at 0, 10, 30, 60, 90 and 120 min. after the glucose load, and blood glucose concentration was measured using a One Touch Ultra glucometer (Johnson&Johnson New Jersey, USA). The area under the curve (AUC) was then determined using the linear method of the trapezoid rule 21 (link),22 (link).
7
Plasma Lipid and Enzyme Profile
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Cholesterol and triglyceride plasma levels [mg/dl] as well as aspartate aminotransferase (AST), alanine transaminase (ALT) and gamma-glutamyl transpeptidase (gGT) activities in plasma [U/l] were measured with Roche automated system (Integra 800, Roche diagnostics).
8
Serum Biomarker Measurement Protocol
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Serum cholesterol, triglycerides, creatinine and urea were measured using an automatic analyser Integra 800 (Roche Diagnostic, Mannheim, Germany). Plasma renin activity was measured with a commercially available radioimmunoassay kit (DiaSorin, Stillwater, MN, USA).
9
Serum Calcium, Phosphorus, and Bone Markers
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Serum calcium, phosphorus, and ALP levels were measured with a spectrophotometric device using a commercial kit (Roche Integra 800). Vitamin D was quantified by high performance liquid chromatography (HPLC) (Vertical Mark of Column device by UFLC-SHIMADZU, with features by Verti SepTM GES C18HPLC Column, ImmuChrom GmbH lot number VD-130218F). Serum levels of PTH were measured by a chemiluminescence immunoassay device (Siemens Centaur XP).
10
Fasting Metabolic Profile Assessment
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Venous blood samples were obtained after a 12-h overnight fast and analyzed using standard automated laboratory methods at the Laboratory of Srinagarind Hospital, Faculty of Medicine, Khon Kaen University, Thailand. The measurements obtained included fasting blood glucose, serum total cholesterol (TC), HDL and LDL (Roche Integra 800, Basel, Switzerland), and hsCRP concentrations (BN ProSpec, Dade Behring Marburg GmbH, United States). In addition, serum insulin concentration was measured using radioimmunoassay with a commercial kit (I125/RIA) from MP Biomedicals, LLC (Irvine, CA, United States). Insulin sensitivity was determined by the homeostatic model assessment of insulin resistance (HOMA-IR) technique described by Matthews et al. (1985) (link), whereby:
Compliance with the target workloads and number of sessions was > 90%.
Compliance with the target workloads and number of sessions was > 90%.
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