Rictor antibody

We used 1:1,000 dilution for all antibodies. Two antibodies (home-made antibody number 1842 and HPA045537 (Sigma, St Louis, MO) against human 4E-BP3 were used. Antibody against human TFE3 (A302-622A) was purchased from Bethyl (Montgomery, TX). Antibodies against 4E-BP1(9644), 4E-BP2(2845), rpS6, phospho-rpS6 (Ser240/244) (2215), Lamin B1 (13435) and α-Tubulin (2144S) were purchased from Cell Signaling Technology (Danvers, MA). Antibodies against eIF4E (610270) and β-Actin (A5441) were from Sigma. Raptor antibody was from Millipore (Billerica, MA). Rictor antibody was from Bethyl. Horseradish peroxidase-conjugated anti-rabbit IgG and anti-mouse IgG were from Amersham Biosciences (Baie d'Urfé, QC, Canada). PP242 and INK1341 were provided by Intellikine (La Jolla, CA, USA).

Antibodies and reagents were obtained from the following sources: the raptor antibody (catalogue No. 09-217) was from Millipore, Schwalbach Germany. mTOR (catalogue No. 2972) and myc-tag (catalogue No. 2272) antibodies were from Cell Signaling Technology, Danvers, MA, USA. The rictor antibody (catalogue No. A300-459A) was from Bethyl Laboratories, Montgomery, TX, USA. hnRNP A2/B1 (catalogue No. sc-32316) and dynamin 2 (catalogue No. sc-6400) was from Santa Cruz Biotechnology, Dallas, TX, USA. Horseradish peroxidase (HRP) labelled secondary antibodies were from Bio-Rad, Munich, Germany. CHAPS buffer was from Applichem, Darmstadt, Germany. The complete protease and phosphatase inhibitor cocktail were from Roche, Mannheim, Germany. Dithiothreitol (DTT), trypsin, trifluoroacetic acid (TFA), formic acid (FA), acetonitrile (ACN) and ammonium bicarbonate (AMBIC) were from Sigma-Aldrich, Steinheim, Germany. RPMI-1640, DMEM, phosphate buffer saline (PBS), penicillin and streptomycin were from PAA Laboratories, Colbe, Germany. The myc-tag Co-IP kit was from Thermo Scientific Pierce, Rockford, IL, USA. The myc-tag raptor pRK5 plasmid was a kind gift of Dr. Doss Sarbassove (The University of Texas, Austin, TX, USA).