Ang 1 7

Manufactured by Bachem

Sourced in Switzerland, United States, Germany, Panama

Ang-(1-7) is a pharmaceutical-grade peptide compound produced by Bachem. It is a seven-amino-acid fragment of the angiotensin protein. Ang-(1-7) is available in high purity for use in research and development applications.

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Lab products found in correlation

Ang 2, by Merck Group (6 mentions) Angii, by Bachem (4 mentions) Losartan, by Merck Group (4 mentions) Apocynin, by Merck Group (3 mentions) D ala7 ang 1 7, by Bachem (2 mentions) Capsaicin, by Merck Group (2 mentions) Tempol, by Merck Group (2 mentions) Sq22536, by Merck Group (2 mentions) Rp adenosine 3 5 cyclic monophosphothionate rp camp dibutyryl camp db camp nω nitro l arginine methyl ester l name, by Merck Group (2 mentions) C57bl 6j mice, by Jackson ImmunoResearch (2 mentions) Sdf 1α, by R&D Systems (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions) Lysotracker red, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Protease inhibitors cocktail, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Penicilin streptomycin, by Thermo Fisher Scientific (1 mentions) Goat anti mouse antibody coupled to alexa 594, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

ANG I (2 citations) D-Ala7-Ang-(1-7) (2 citations) D-Pro7-Ang-(1-7) (2 citations)

31 protocols using ang 1 7

Pharmacological Characterization of NPFF and Ang(1-7) Ligands

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Non-peptide ligands AR234960 (AR-agonist) and AR244555 (AR-inverse agonist) were a gift from Arena Pharmaceuticals, Inc. (San Diego, CA). Neuropeptide FF (NPFF) was initially purchased from Bachem (King of Prussia, PA). NPFF and NPFF analogs [Y¹]-NPFF, NPFF-C were synthesized by the Molecular Biotechnology Core at Lerner Research Institute (Cleveland, OH). The NPFF analog [^DY¹] [^NMeF³]-NPFF was obtained from Bachem (King of Prussia, PA). Ang(1–7) and angiotensin metabolites were obtained from multiple sources. Ang(1–7) was purchased from Bachem (King of Prussia, PA), Sigma-Aldrich (St. Louis, MO) and Phoenix Pharmaceuticals (Burlingame, CA). Ang(1–7)-amide was purchased from Labpe Chemicals (Houston, TX). Ang(1–7) analogs ([^DA⁷]-Ang(1–7) (A779) and [Sar¹]-Ang(1–7)-amide) and angiotensin metabolites (AngIII, AngIV/Ang(3–8) and Ang(3–7)) were purchased from Bachem (King of Prussia, PA). AngIV-amide was synthesized by the Molecular Biotechnology core at Lerner Research Institute (Cleveland, OH). Pan-inhibitor of G-protein signaling, BIM-46187, was a gift from IPSEN Innovation (Les Ulis, France).

Tirupula K.C., Desnoyer R., Speth R.C, & Karnik S.S. (2014). Atypical Signaling and Functional Desensitization Response of MAS Receptor to Peptide Ligands. PLoS ONE, 9(7), e103520.

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Angiotensin II-Induced Cell Injury Assay

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Ang II (Sigma Aldrich, MO) induced cell injury model was produced as previously reported [20 (link)–22 (link)]. In brief, confluent HbmECs were incubated with increasing concentrations of Ang II (10⁻⁹, 10⁻⁸, 10⁻⁷ and 10⁻⁶ M) for 24 h. After that, cells were collected for annexin V measurements. In order to determine the effective concentration of Ang-(1–7) (Bachem AG, CH) to suppress Ang II-induced pro-apoptotic activity, HbmECs were pre-treated with different concentrations of Ang-(1-7) (10⁻⁹, 10⁻⁸, 10⁻⁷ and 10⁻⁶ M) for 30 min before addition of Ang II (10⁻⁷ M) for 24 h. After that, HbmECs were collected for annexin V measurements. Upon completion of this study, we chose 10⁻⁷ M of Ang II and Ang-(1-7) for the subsequent experiments.

Xiao X., Zhang C., Ma X., Miao H., Wang J., Liu L., Chen S., Zeng R., Chen Y, & Bihl J.C. (2015). Angiotensin-(1-7) Counteracts Angiotensin II-induced Dysfunction in Cerebral Endothelial Cells via Modulating Nox2/ROS and PI3K/NO Pathways. Experimental cell research, 336(1), 58-65.

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Ang-(1-7) Modulates CD34+ Cell Migration

Cited 1 time

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CD34⁺ cell migration toward 100 nM stromal cell-derived factor 1α (SDF-1α, R&D Systems, Minneapolis, MN) or PBS for 4 h at 37°C was measured using the QCM Chemotaxis 5 μm Migration Chamber (Millipore, Billerica, MA) according to manufacturer instructions and as previously described^{18 (link)}. For determination of the effects of Ang-(1-7) on migration, freshly isolated CD34⁺ cells were pretreated with 100 nM Ang-(1-7) (Bachem, Bubendorf, Switzerland) for 1 h before following protocol above. This concentration of Ang-(1-7) was selected based on a preliminary dose-response experiment and previous experience with treatment of CD34⁺ cells with Ang-(1-7) in vitro^{16 (link)}.

Cole-Jeffrey C.T., Pepine C.J., Katovich M.J., Grant M.B., Raizada M.K, & Hazra S. (2018). Beneficial effects of Angiotensin-(1-7) on CD34+ cells from heart failure patients. Journal of cardiovascular pharmacology, 71(3), 155-159.

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Analyzing Angiotensin Receptor Trafficking

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Fetal bovine serum, penicilin-streptomycin, Lipofectamine 2000, goat anti-mouse antibody coupled to Alexa 594 and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Invitrogen (Carlsbad, CA, USA). Bovine seroalbumin, paraformaldehyde, phosphate buffered saline (PBS) and the protease inhibitors cocktail were from Sigma Chemical Co. (St. Louis, MO, USA). Mouse anti Rab4, Rab11 or anti-CAV-1 antibodies were purchased from BD Biosciences. LysoTracker Red was from Molecular Probes. Ang-(1-7) was from Bachem. The plasmid containing MAS1R-YFP cDNA was obtained as previously described.^{17 (link)} shRNA ß-arrestin2 was from SantaCruz. All other chemicals were analytical grade reagents of the highest purity available.

Cerniello F.M., Carretero O.A., Carbajosa N.L., Cerrato B.D., Santos R.A., Grecco H.E, & Gironacci M.M. (2017). MAS1 Receptor Trafficking Involves ERK1/2 Activation through a β-arrestin2-dependent Pathway. Hypertension (Dallas, Tex. : 1979), 70(5), 982-989.

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Ang-(1-7) Peptide Purification and Endotoxin Testing

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Ang-(1-7) was purchased as a synthetic peptide from Bachem Inc., and purity (> 99%) was checked by the company using high-performance liquid chromatography. The peptide was diluted in endotoxin-free PBS, and solutions were tested by the Limulus amebocyte lysate endotoxin assay (Pierce). LPS contamination was insignificant (<0.5 endotoxin units/mL or <0.05 ng).

Zaidan I., Tavares L.P., Sugimoto M.A., Lima K.M., Negreiros-Lima G.L., Teixeira L.C., Miranda T.C., Valiate B.V., Cramer A., Vago J.P., Campolina-Silva G.H., Souza J.A., Grossi L.C., Pinho V., Campagnole-Santos M.J., Santos R.A., Teixeira M.M., Galvão I, & Sousa L.P. (2022). Angiotensin-(1-7)/MasR axis promotes migration of monocytes/macrophages with a regulatory phenotype to perform phagocytosis and efferocytosis. JCI Insight, 7(1), e147819.

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Vasopressor Signaling Pathway Modulation

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All chemicals were dissolved in normal saline to create working solutions at stated concentrations. Ang-(1-7) and D-alanine-Ang-(1-7) (A-779, an antagonist of Mas receptors) were obtained from Bachem (Bubendorf, Switzerland). Ang II, losartan, prostaglandin F2α (PGF 2α), and acetylcholine (ACh) were purchased from Sigma Chemical Co. (St. Louis, MO, USA).

Zhang F., Xu Y., Pan Y., Sun S., Chen A., Li P., Bao C., Wang J., Tang H, & Han Y. (2019). Effects of Angiotensin-(1-7) and Angiotensin II on Acetylcholine-Induced Vascular Relaxation in Spontaneously Hypertensive Rats. Oxidative Medicine and Cellular Longevity, 2019, 6512485.

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Angiotensin II-Induced HBVSMC Responses

Cited 2 times

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HBVSMCs (Sciencell Research Laboratories, Carlsbad, CA, USA) were cultured in 75-mm²flasks in SMC medium, supplemented with 2% fetal bovine serum, 100 U/ml penicillin and streptomycin, and 100 U/ml cell growth supplement (Sciencell Research Laboratories) in a 37°C incubator with humidified atmosphere of 5% CO2/95% air. After reaching confluence, cells were randomly subjected to different treatments for 24 hrs (Figure 1): vehicle, Ang II, Ang II + Ang-(1-7), Ang II + A-779, Ang II + Ang-(1-7) + A-779. The concentration of Ang II (10⁻⁶ M, Sigma-Aldrich, St. Louis, MO) was chosen according to our previous study [15 (link)]. The dose of Ang-(1-7) (10⁻⁷ M, Bachem AG) was determined based on the concentration-response study (Figure 2A). A-779 (Mas receptor antagonist, 1 μM, BachemAG, Torrance, CA, USA) was pre-incubated with cells for 30 min [16 (link)].

Bihl J.C., Zhang C., Zhao Y., Xiao X., Ma X., Chen Y., Chen S., Zhao B, & Chen Y. (2015). Angiotensin-(1-7) Counteracts the Effects of Ang II on Vascular Smooth Muscle Cells, Vascular Remodeling and Hemorrhagic Stroke: Role of the NFκB Inflammatory Pathway. Vascular pharmacology, 73, 115-123.

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Radioimmunoassay Protocol for Labeled Peptides

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The radioimmunoassay requires preparation of
¹²⁵I-labeled peptides.

Iodine-125 10 mCi per 0.1 ml (Perkin Elmer
NEZ033A010MC).

MilliQ Plus ultra pure water system with distilled
water input (Millipore Q Pak cartridges #CPMO004D2).

Chloramine T (Sigma #C9887) 1 mg/ml in MilliQ
ultra-pure water, prepare fresh.

Sodium metabisulfite (Sigma #S-9000) 3 mg/ml in
MillQ water, prepare fresh.

Phosphate buffered saline (PBS).

Ang-(1–7) (Bachem) 1 mM in MillQ water.

SepPak columns: Sep-Pak C18 3 cc Vac cartridges (Waters
#WAT020805).

Trifluoroacetic acid (TFA, HPLC grade, Pierce CAS 76
05-01) 0.1 % solution. 0.1 ml TFA in 100 ml MillQ
water.

Methanol (HPLC grade, Fisher #A412),
80% solution in 0.1 % TFA. Add 0.1 ml TFA to 80
ml methanol and bring to a final volume of 100 ml with MilliQ
water.

15 ml conical tubes (Starstedt
#62.554.002).

Brosnihan K.B, & Chappell M.C. (2017). Measurement of Angiotensin Peptides: HPLC-RIA. Methods in molecular biology (Clifton, N.J.), 1527, 81-99.

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Pharmacological Modulation of Ang-(1–7) Axis

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The Mas receptor antagonist A779 (Asp-Arg-Val-Tyr-Ile-His-[D-Ala]) and the specific ACE2 inhibitor DX600 (Ac-Gly-Asp-Tyr-Ser-His-Cys-Ser-Pro-Leu-Arg-Tyr-Tyr-Pro-Trp-Trp-Lys-Cys-Thr-Tyr-Pro-Asp-Pro-Glu-Gly-Gly-Gly-NH2), were purchased from Phoenix Pharmaceuticals Inc. (St. Joseph, MO). Ang-(1–7) was purchased from Bachem Americas (Torrance, CA) and diphenyleneiodonium chloride (DPI) was purchased from Sigma (St. Louis, MO).

Wang L., Liang J, & Leung P.S. (2015). The ACE2/Ang-(1-7)/Mas Axis Regulates the Development of Pancreatic Endocrine Cells in Mouse Embryos. PLoS ONE, 10(6), e0128216.

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Human Endothelial Cell Culture Protocol

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M199 culture medium, foetal calf serum (FCS) was from Biological Industries (Beit-Hamek, Israel). Heparin, endothelial cell growth supplement (ECGS), amphotericin, type II collagenase, type I collagen, EDTA, sodium orthovanadate, phenylmethylsulfonyl fluoride (PMSF) were purchased from Sigma (St. Louis, MO, USA). IL-1β and human recombinant klotho (r-klotho) were purchased from Preprotech (London, UK) and Abcam (ab84072; Cambridge, UK), respectively, while Ang-(1-7) was purchased from Bachem (Bubendorf, Switzerland). The sodium salt CP-456773 (also known as MCC 950) was purchased from Sigma (St. Louis, MO, USA). Anakinra was obtained from Biovitrum (SOBI, Stockholm, Sweden).

Romero A., Dongil P., Valencia I., Vallejo S., Hipólito-Luengo Á.S., Díaz-Araya G., Bartha J.L., González-Arlanzón M.M., Rivilla F., de la Cuesta F., Sánchez-Ferrer C.F, & Peiró C. (2022). Pharmacological Blockade of NLRP3 Inflammasome/IL-1β-Positive Loop Mitigates Endothelial Cell Senescence and Dysfunction. Aging and Disease, 13(1), 284-297.

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