Infinite m200 pro fluorescent microplate reader

SKBR3, ZR75 and MCF10A cell lines (10,000 cells/well) were seeded on clear bottom 96-well plates (Thermo Fisher Scientific, USA), cultured in their respective media (100 µl/well) and were left to adhere overnight.
Cells were treated with different concentrations of PAMAM dendrimers, ranging from 0.1 to 100 µM. Additionally, lapatinib; a well-known anti-HER2 drug, was used as a control. Based on previous studies, lapatinib treatment was given in the concentrations of 10 to 100 nM in SKBR3, and 1 to 100 µM in ZR75 [27] (link), [28] (link), [29] (link). Cells were treated at three different time-points (24, 48, and 72 h). Control wells received 100 μl of media (control). Alamar Blue Cell viability reagent (Invitrogen, Thermo Fisher Scientific, USA) was used to determine cell viability, according to the manufacturer’s protocol. Briefly, 2% Alamar Blue dye was added to the plates, followed by incubation for 3–4 h. Post-incubation, fluorescence was recorded at a wavelength of 560 nm (excitement) and 600 nm (emission) using Infinite m200 PRO fluorescent microplate reader (TECAN, Switzerland).

SKBR3 and ZR75 cells were seeded in 96-well plates (Thermo Fisher Scientific, Mississauga, ON, Canada) in a density of 1 × 10⁴ cells/well. Cells were treated with different concentrations of DA (1, 5, 7, 10, 15, and 20 µM) and BMS-202 (1, 5, 7, 10, 15, 20, and 30 µM) for 48 h. A combined treatment of DA and BMS-202 was prepared at various concentrations (1, 2, 3, 5, 7, 10, and 20 µM) and used on both cell lines for 48 h. The concentration of DMSO did not exceed 0.1%. Control wells received 100 µl of media. According to the manufacturer’s protocol, cell viability inhibition was determined after 48 h of treatment using Alamar Blue Cell viability reagent (Invitrogen, Thermo Fisher Scientific). After 4 hours of incubation with the dye, the shifts in fluorescence were recorded at a wavelength of 560 nm (excitation) and 600 nm (emission) using the Infinite m200 PRO fluorescent microplate reader (TECAN, Männedorf, Switzerland). Relative cell viability was determined based on the fluorescence of drug-treated cells compared with that of control cells.