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Human interleukin 35 il35 elisa kit

Manufactured by Nordic Biosite
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The Human Interleukin 35 (IL35) ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the detection and quantification of human IL35 in various biological samples.

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Lab products found in correlation

Human tgf beta 1 elisa kit, by Abcam (2 mentions) Softmax pro 7, by Molecular Devices (2 mentions) Spectra max plus 384 microplate spectrophotometer, by Molecular Devices (2 mentions) Quantikine hs elisa human il10, by RnD Systems (2 mentions)

2 protocols using human interleukin 35 il35 elisa kit

1

Treg-specific Cytokine Quantification

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The Quantikine HS ELISA Human IL10 (RnD Systems, cat. HS100C), Human TGF beta 1 ELISA kit (Abcam, cat. ab100647) and Human Interleukin 35 (IL35) ELISA Kit (Nordic BioSite, cat. EKX-6FHVKH-96) were used to measure the amount of Treg-specific cytokines in Treg suppression assay supernatant, according to instructions from the manufacturers. For IL35, samples were diluted 1:1 in sample dilution buffer, for IL10 and TGF- β , samples were handled according to manufacturer’s protocols. Absorbance was read at A450 nm (IL35 and TGF- β ), and A490 nm (IL10) using the SpectraMax plus 384 Microplate Spectrophotometer and SoftMax Pro 7.1 software (Molecular Devices).
Sjøgren T., Islam S., Filippov I., Jebrzycka A., Sulen A., Breivik L.E., Hellesen A., Jørgensen A.P., Lima K., Tserel L., Kisand K., Peterson P., Ranki A., Husebye E.S., Oftedal B.E, & Wolff A.S. (2024). Single cell characterization of blood and expanded regulatory T cells in autoimmune polyendocrine syndrome type 1. iScience, 27(4), 109610.
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2

Quantification of Treg-Specific Cytokines

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Treg-specific cytokines were measured in supernatant from the suppression assay at the end of culturing using Quantikine HS ELISA Human IL-10 (RnD Systems, cat. HS100C), Human TGF beta 1 ELISA Kit (Abcam, cat. AB100647), and Human Interleukin 35 (IL-35) ELISA Kit (Nordic BioSite, cat. EKX-6FHVKH-96) according to instructions from the manufacturers. Absorbance was read at A450 nm (IL-35 and TGF-β), and A490 nm (IL-10) using a SpectraMax plus 384 Microplate Spectrophotometer and the SoftMax Pro 7.1 software (Molecular Devices).
Sjøgren T., Bjune J.I., Husebye E.S., Oftedal B.E, & Wolff A.S. (2023). Regulatory T cells in autoimmune primary adrenal insufficiency. Clinical and Experimental Immunology, 215(1), 47-57.
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