The levels of TC (cat. no. CS0005; Sigma-Aldrich; Merck KGaA), TG (cat. no. MAK266; Sigma-Aldrich; Merck KGaA) and LDL-r (cat. no. RAB0707; Sigma-Aldrich; Merck KGaA) were measured using ELISA kits. An Oil Red O staining kit (cat. no. ab150678; Abcam) was also employed. The primary antibodies were anti-β-actin (cat. no. ab8227; 1:4,000; Abcam), anti-SRA1 (1:500; cat. no. sc-166139; Santa Cruz Biotechnology, Inc.), anti-CD36 (1:3,500; cat. no. ab133625; Abcam), anti-SREBP2 (1:3,000; cat. no. ab30682; Abcam) and anti-LDL-r (1:4,000; cat. no. ab52818; Abcam). The secondary antibodies used were Goat IgG horseradish peroxidase (HRP)-conjugated antibody (1:2,000; cat. no. HAF017; R&D Systems, Inc.) and Rabbit IgG HRP-conjugated antibody (1:2,000; cat. no. HAF008; R&D Systems, Inc.).
Anti sra1
Manufactured by Abcam
Sourced in United Kingdom
Anti-SRA1 is a protein-specific antibody that recognizes the SRA1 (Steroid Receptor RNA Activator 1) protein. SRA1 is a non-coding RNA that functions as a transcriptional coactivator for various nuclear receptors.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd36, by Santa Cruz Biotechnology (1 mentions)
Anti ldlr, by Abcam (1 mentions)
Anti srebp2, by Abcam (1 mentions)
Rabbit igg hrp conjugated antibody, by R&D Systems (1 mentions)
Anti chop, by Cell Signaling Technology (1 mentions)
Huh7 cell line, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti sr b1, by Novus Biologicals (1 mentions)
Anti α tubulin, by GeneTex (1 mentions)
Sodium palmitate, by Merck Group (1 mentions)
Anti cd36, by GeneTex (1 mentions)
Anti α sma, by GeneTex (1 mentions)
N acetylcysteine, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
2 protocols using anti sra1
1
Lipid Metabolism Pathway Analysis
2
Hepatocyte Lipid Metabolism Assay
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BPA, bovine serum albumin (BSA), sodium oleate (OA), sodium palmitate (PA), n-acetylcysteine (NAC), and phosphate-buffered saline (PBS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). BODIPY FL lactosylceramide (LacCer) complexed to BSA was purchased from Thermo Fisher Scientific (Waltham, MA, USA). The antibodies used included anti-CD36 (GeneTex, Irvine, CA, USA), anti-α-SMA (GeneTex), anti-α-tubulin (GeneTex), anti-collagen I (GeneTex), anti-CHOP (Cell Signaling Technology, Danvers, MA, USA), anti-cleaved caspase-3 (Cell Signaling Technology), anti-SR-A1 (Abcam, Cambridge, UK), and anti-SR-B1 (Novus Biologicals, Littleton, CO, USA).
A human hepatoma HUH-7 cell line was purchased from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). HUH-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich) and a 1% mixture of penicillin G, streptomycin, and amphotericin B at 37 °C in a 5% CO2 incubator.
A human hepatoma HUH-7 cell line was purchased from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan). HUH-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich) and a 1% mixture of penicillin G, streptomycin, and amphotericin B at 37 °C in a 5% CO2 incubator.
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