Briefly, 1,25(OH)2D3, ACET, acetaminophen glucuronide (AG), BUP, hydroxybupropion (HBUP), carbamazepine (CARB), diltiazem (DIL), theophylline (THEO), TOL, 4-hydroxytolbutamide (HTOL), and uridine diphosphate glucuronic acid (UDPGA) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Dihydronicotinamide adenine dinucleotide phosphate (NADPH) was purchased from Corning, Inc. (New York, NY, USA). All solvents used were of HPLC grade. The PrimeScriptTM 1st strand cDNA Synthesis Kit and SYBR® Premix Ex TaqTM II ROX plus were purchased from Takara Bio, Inc. (Shiga, Japan). Forward and reverse primers for quantitative polymerase chain reaction (qPCR) experiment were synthesized by Bioneer Co. (Daejeon, Korea).
Acetaminophen glucuronide
Manufactured by Merck Group
Sourced in United States
Acetaminophen glucuronide is a laboratory reagent used in analytical and research applications. It is a conjugate of acetaminophen and glucuronic acid, commonly used as an internal standard or reference material in the analysis of acetaminophen and its metabolites.
Automatically generated - may contain errors
Lab products found in correlation
Diltiazem, by Merck Group (1 mentions)
Uridine diphosphate glucuronic acid, by Merck Group (1 mentions)
Hydroxybupropion, by Merck Group (1 mentions)
Theophylline, by Merck Group (1 mentions)
4 hydroxytolbutamide, by Merck Group (1 mentions)
Primescripttm 1st strand cdna synthesis kit, by Takara Bio (1 mentions)
Carbamazepine, by Merck Group (1 mentions)
Metaphosphoric acid, by Merck Group (1 mentions)
Trimethylchlorosilane, by Merck Group (1 mentions)
Allopurinol, by Merck Group (1 mentions)
High purity trifluoroacetic acid, by Merck Group (1 mentions)
Salicyluric acid, by Merck Group (1 mentions)
Catechol, by Merck Group (1 mentions)
2 4 6 tris 2 pyridyl 1 3 5 triazine, by Merck Group (1 mentions)
2 acetamidophenol, by Merck Group (1 mentions)
3 phenylbutyric acid, by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Caffeine, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Hydrogen peroxide, by Merck Group (1 mentions)
3 protocols using acetaminophen glucuronide
1
Quantitative Analysis of Drug Metabolites
2
Comprehensive Metabolite Quantification
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Caffeine, allopurinol, acetaminophen-glucuronide, acetaminophen-sulphate, high purity trifluoroacetic acid, salicyluric acid, 3-phenyl butyric acid, N,N-diethyl-paraphenylenediamine (DEPPD), sodium acetate, ammonium acetate, hydrogen peroxide, 2-acetamidophenol, 2,3 and 2,5 dihydroxy benzoic acid, catechol, N,O Bis(trimethylsilyl)fluoro acetamide (BSTFA), trimethylchlorosilane (TMCS), hydrochloric acid, sodium sulfate, pyridine, 2,4,6-tris-2-(pyridyl)- 1,3,5-triazine (TPTZ), Ferri Chloride (FeCl3·6H2O), and metaphosphoric acid (MPA) were all obtained from Sigma-Aldrich (now Merck). Acetaminophen-mercapturate was supplied by Toronto Research Chemicals. HPLC grade acetonitrile, methanol, and water was supplied by Burdick & Jackson. Ferrous sulphate was supplied by Labchem. Ethyl acetate and diethyl ether, formic acid, acetic acid, and butanoic HCL were purchased from Merck. Carnitine and acylcarnitine reference standards and deuterated analogues were obtained from Cambridge Isotope Laboratories. GSH/GSSG-412 assay kits were purchased from Aoxre Bio-Sciences.
3
Analytical Validation of Glucuronide Assays
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All animal experiments were performed under the supervision and with the approval of the Institutional Animal Care and Use Committee of Hokkaido University, Japan (approval number 14-0119), which conform to the Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC).
Chemicals. 1-Hydroxypyrene, pyrene glucuronide, Acetaminophen glucuronide, β-estradiol, sodium cholate hydrate, UDPGA, and RNA later ® were obtained from Sigma-Aldrich (St.
Louis, MO). Acetaminophen, acetic acid, sodium phosphate, and ammonium acetate solution were purchased from Wako Pure Chemical Industries Ltd. (Osaka, Japan). β-estradiol-3-β-Dglucuronide sodium salt was obtained from Santa-Cruz Biotechnology Inc. (Santa-Cruz, CA).
All chemicals used for high-performance liquid chromatography (HPLC) and mass spectrometry (MS) were HPLC or MS grade and were obtained from Kanto Chemical Co. Inc.
(Tokyo, Japan).
Chemicals. 1-Hydroxypyrene, pyrene glucuronide, Acetaminophen glucuronide, β-estradiol, sodium cholate hydrate, UDPGA, and RNA later ® were obtained from Sigma-Aldrich (St.
Louis, MO). Acetaminophen, acetic acid, sodium phosphate, and ammonium acetate solution were purchased from Wako Pure Chemical Industries Ltd. (Osaka, Japan). β-estradiol-3-β-Dglucuronide sodium salt was obtained from Santa-Cruz Biotechnology Inc. (Santa-Cruz, CA).
All chemicals used for high-performance liquid chromatography (HPLC) and mass spectrometry (MS) were HPLC or MS grade and were obtained from Kanto Chemical Co. Inc.
(Tokyo, Japan).
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