Malvern zetasizer 7

¹H NMR (400 MHz), ³¹P NMR (161.92 MHz) and ¹⁹F NMR (376.03 MHz) were recorded using an Ascend Bruker NMR spectrometer (Bruker Biospin Corp, Billerica, MA, USA). Dynamic light scattering (DLS) measurements were conducted using a Malvern Zetasizer Nano series (Malvern Panalytical, Malvern, UK) and data analysis was performed using Malvern Zetasizer 7.10 software (Malvern Instruments Ltd., Worcestershire, UK). Fluorescence spectra were recorded using a BioTek synergy neo2 multi-mode reader (BioTek Instruments, Inc., Winooski, VT, USA). UV-vis spectral analysis was conducted using a NanoDrop2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). Size-exclusion chromatography (SEC) analysis of molecular masses and polymer degradation studies were conducted using an Agilent 1260 Infinity II Binary LC system equipped with a G7112B binary pump, G7167A Multisampler, G7116A multicolumn thermostat, G7117C diode array detector, G7121A fluorescence detector (Agilent Technologies, Inc., Santa Clara, CA, USA) and TSKgel GMPW size-exclusion column (Tosoh Bioscience, LLC, King of Prussia, PA, USA); PBS with 10% acetonitrile was used as a mobile phase.

Dynamic light scattering (DLS) was performed using a Zetasizer Nano series, ZEN3600 (Malvern Instruments Ltd., Worcestershire, UK) and analyzed using Malvern Zetasizer 7.10 software (Malvern Instruments Ltd., Worcestershire, UK). Samples were prepared in PBS, pH 7.4 at a concentration of 1 mg/mL and filtered using Millex 0.22 μm filters (Millipore Sigma) prior to the analysis.