Log In Sign up
The largest database of trusted experimental protocols

Anti ccr7

Manufactured by BioLegend
Visit Supplier
Sourced in United States

Anti-CCR7 is a lab equipment product that recognizes the CCR7 chemokine receptor. CCR7 is involved in the trafficking of lymphocytes, particularly T cells and dendritic cells, to secondary lymphoid organs. This product can be used to study the expression and function of CCR7 in various biological and experimental systems.

Automatically generated - may contain errors

Lab products found in correlation

Anti cd3, by BioLegend (4 mentions) Anti cd45ra, by BioLegend (4 mentions) Anti cd4, by BioLegend (4 mentions) Anti cd8, by BioLegend (4 mentions) Anti hla dr, by BioLegend (4 mentions) Anti cd86, by BioLegend (3 mentions) Anti cd127, by BioLegend (3 mentions) Anti cd45, by BioLegend (2 mentions) Anti cd62l, by BioLegend (2 mentions) Anti cd80, by BioLegend (2 mentions) Anti cd11c, by BioLegend (2 mentions) Anti cd11b, by BioLegend (2 mentions) Anti cd45ra, by BD (2 mentions) Efluor 455uv fixable live dead stain, by Thermo Fisher Scientific (2 mentions) Lsrfortessa x 20, by BD (2 mentions) Anti ccr6, by BioLegend (2 mentions) Anti pd 1, by BD (2 mentions) Anti ccr4, by BioLegend (2 mentions) Anti cd44, by BioLegend (2 mentions) Anti cd3, by BD (2 mentions)

Spelling variants of this product

Anti-CCR7-PE-Cy7 (11 citations) Anti-CCR7-BV785 (4 citations) Anti-CCR7-PerCP/Cy5.5 (3 citations) Anti-CCR7-FITC (3 citations) APC-Cy7 anti-human CCR7 (2 citations) Anti-CCR7-FITC (clone G043H7, (2 citations) APC-conjugated anti-CCR7 (2 citations) Anti-CCR7 (G043H7, (2 citations) Alexa Fluor 488-conjugated anti-CCR7 (2 citations) Peridinin-chlorophyll protein-Cy5.5 anti-CCR7 (2 citations)

13 protocols using anti ccr7

1

Comprehensive Immunophenotyping of LNMC and PBMC

Check if the same lab product or an alternative is used in the 5 most similar protocols
Two million of LNMC or PBMC were stained for flow cytometry as previously described to analyze the expression of surface and intracellular markers as well as cellular composition using the 3-laser FACS Fortessa (Becton Dickinson; San Jose, USA)(16 (link)). Antibodies used in these analyses were purchased from either BD or Biolegend and included anti-CD16 (clone 3G8), anti-CD11b (clone M1/70), anti-CD1c (clone L161), anti-CD8 (clone SK1), anti-CD14 (clone M5E2), anti-CD11c (clone 3.9), anti-CD4 (clone OKT4), anti-CD20 (clone 2H7), anti-CD123 (clone 7G3), anti-HLA-DR (clone Immu-357), anti-CD3 (clone SP34–2), anti-CCR6 (clone GO34E3), anti-PD1 (clone EH12.2H7), anti-CD28 (clone 28.2), anti-CD69 (clone FN50), anti-CD95 (clone DX2), anti-CDCXCR3 (clone G025H7), anti-CD25 (clone M-A251), anti-Ki67 (clone B56), anti-CXCR5 (clone 1C6), and anti-CCR7 (clone 3D12). A minimum of 100,000 CD3+ T cells were collected, and post-acquisition analysis performed by Y.C. using FlowJo (Version 9.6, TreeStar) software.
CAI Y., Watkins M.A., Xue F., Ai Y., Cheng H., Midkiff C.C., Wang X., Alvarez X., Poli A.N., Salvino J.M., Veazey R.S, & Montaner L.J. (2019). BCL6 BTB-specific Inhibition via FX1 Treatment Reduces Tfh Cells & Reverses Lymphoid Follicle Hyperplasia in Indian Rhesus Macaque (Macaca mulatta). Journal of medical primatology, 49(1), 26-33.
+ Open protocol
+ Expand
2

Phenotyping Peripheral Blood T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peripheral blood mononuclear cells (PBMCs) were isolated by centrifugation on Ficoll. PBMCs were stained with anti-CD45, anti-CD3, anti-CD4, anti-CD8, anti-CD45RA, anti-CCR7 and anti-CD62L antibodies (all from Biolegend) and analyzed on a LSR II Fortessa (BD Biosciences). Analysis of relative frequencies was done using FlowJo v10 (FlowJo LLC).
Läubli H., Balmelli C., Kaufmann L., Stanczak M., Syedbasha M., Vogt D., Hertig A., Müller B., Gautschi O., Stenner F., Zippelius A., Egli A, & Rothschild S.I. (2018). Influenza vaccination of cancer patients during PD-1 blockade induces serological protection but may raise the risk for immune-related adverse events. Journal for Immunotherapy of Cancer, 6, 40.
+ Open protocol
+ Expand
3

Phenotypic Analysis of Surface DCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
For flow cytometry analysis of surface DC phenotype, we used the following monoclonal antibodies (mAb): FITC-labeled anti-CD1c (Biolegend, San Diego, CA, USA), anti-CD14, anti-CD16 and anti-HLA I (all from Invitrogen, Camarillo, CA, USA), Alexa Fluor 488-labeled anti-CD1a and anti-CCR7 (both Biolegend). PE-labeled antibodies included: anti-CD11b, anti-CD11c (both Biolegend), anti-CD80, anti-CD86, anti-DC-SIGN, (all Biolegend), and anti-HLA DR (Miltenyi Biotec, Bergisch Gladbach, Germany).
DCs differentiated in Cellgenix® DC GMP medium (Cellgenix GmbH, Freiburg, Germany) were harvested and collected by centrifugation. Before staining, the cells were washed twice in DPBS in all cases. Antibody was added and the cells were incubated for 15 min in the dark, then washed twice and resuspended in 2% paraformaldehyde (PHA). Samples were analyzed on a FACSCalibur system (BD biosciences, Franklin Lakes, NJ, USA). Data were analyzed with CellQuest software (BD biosciences).
Fevžer T., Poženel P., Zajc K., Tešić N, & Švajger U. (2022). Combined TLR-3/TLR-8 Signaling in the Presence of α-Type-1 Cytokines Represents a Novel and Potent Dendritic Cell Type-1, Anti-Cancer Maturation Protocol. Cells, 11(5), 835.
+ Open protocol
+ Expand
4

Longitudinal Immunophenotyping of PBMC

Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMC samples were obtained from patients before the administration of αGalCer-pulsed APCs, and another eight times during the 11-week study period (figure 1). mAbs to detect and fractionate mononuclear cells in peripheral blood were: FITC-conjugated anti-TCR Vα24 (Beckman Coulter, Brea, CA), anti-CD14 (BD Biosciences), anti-CD45RA (BD Biosciences), PE-conjugated anti-TCR Vβ11 (Beckman Coulter), anti-CD56 (BD Biosciences), anti-CCR7 (Biolegend), allophycocyanin-conjugated anti-CD3 (BD Biosciences), αGalCer-loaded CD1d tetramer (ProImmune), allophycocyanin-Cy7 conjugated anti-CD3 (BD Biosciences), PE-Cy7-conjugated anti-CD8 (BD Biosciences), PB-conjugated anti-CD4 (BD Biosciences), anti-CD16 (BD Biosciences), anti-PD-1 (Biolegend), and anti-HLA-DR(Biolegend). The absolute numbers of these cells were also calculated using automated full blood counts.
Toyoda T., Kamata T., Tanaka K., Ihara F., Takami M., Suzuki H., Nakajima T., Ikeuchi T., Kawasaki Y., Hanaoka H., Nakayama T., Yoshino I, & Motohashi S. (2020). Phase II study of α-galactosylceramide-pulsed antigen-presenting cells in patients with advanced or recurrent non-small cell lung cancer. Journal for Immunotherapy of Cancer, 8(1), e000316.
+ Open protocol
+ Expand
5

Phenotyping of T-cell Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMCs (5 × 105) were resuspended in 100 μL 1X phosphate buffered saline and stained with eFluor 455UV fixable live-dead stain (Thermo Fisher Scientific, Carlsbad, CA), to stain nonviable cells. The cells then were surface stained with anti-CD3, anti-CD4, anti-CD8, anti-CD45RA, anti-CCR7, anti-CD127, anti-CD25, and anti-PD1 fluorochrome conjugated antibodies (BioLegend, San Diego, CA) at room temperature for 30 min to identify the T-cell population and their memory or effector phenotypes. Cells were washed, and acquisition was carried out with a cell analyzer (BD LSRFortessaX-20; BD Biosciences, San Jose, CA). Data analyses were carried out with FlowJo data analysis software (FlowJo version 10.4.2, USA).
Packiriswamy N., Upreti D., Zhou Y., Khan R., Miller A., Diaz R.M., Rooney C.M., Dispenzieri A., Peng K.W, & Russell S.J. (2020). Oncolytic measles virus therapy enhances tumor antigen-specific T-cell responses in patients with multiple myeloma. Leukemia, 34(12), 3310-3322.
+ Open protocol
+ Expand
6

Phenotypic Characterization of Dendritic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell phenotype staining was performed using the following antibodies: mouse anti-human MGL (HML clone, MoAb MLD-1 [25 (link)]; a generous gift from Professor Tatsura Irimura), mouse anti-human MUC1 (HMFG2), mouse anti-STn (TKH2,) followed by FITC-conjugated goat anti-mouse IgG (H+L; DAKO). MoAbs directly conjugated with FITC or phycoerythrin were also used (all from Beckman Coulter unless stated): IgG1-PE or FITC as isotype controls, anti-MGL (Biolegend; clone H037G3), anti-CD1a, anti-CD83, anti-CD80, anti-CD86 (all PE), anti-CD14, anti-HLA-DR, anti-CCR7, anti-CD40 (all FITC). Cells were suspended in PBS + 0.5% BSA (2 × 105 cells/100 μL/sample) and incubated with MoAbs according to the manufacturer's instructions. At least 1 × 104 events were evaluated using either Epics XL, (Beckman Coulter) or FACSCalibur (BD Biosciences) flow cytometers. Analysis was performed using either WinMDI or Cellquest software.
Beatson R., Maurstad G., Picco G., Arulappu A., Coleman J., Wandell H.H., Clausen H., Mandel U., Taylor-Papadimitriou J., Sletmoen M, & Burchell J.M. (2015). The Breast Cancer-Associated Glycoforms of MUC1, MUC1-Tn and sialyl-Tn, Are Expressed in COSMC Wild-Type Cells and Bind the C-Type Lectin MGL. PLoS ONE, 10(5), e0125994.
+ Open protocol
+ Expand
7

Comprehensive Immunophenotyping of T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell culture media was RPMI 1640 (Mediatech Inc.) supplemented with 10% heat-inactivated FBS, 100 U/ml penicillin, 100 mg/ml streptomycin, 2 mM L-glutamine, and 50 mM 2-mercaptoethanol (Sigma-Aldrich). Fluorescent anti-CD4, anti-CD8α, anti-CD8β, anti-CD25, anti-CD44, anti-CD45, anti-CD45.1, anti-CD45.2, anti-CD62L, anti-CD103, anti-CCR4, anti-CCR6, anti-CCR7, anti-CCR-9, anti-TCRβ and anti-TCRγδ antibodies were purchased from Biolegend. Anti-CTLA-4, anti-PD-1, anti-GITR, anti-CD127, anti-IFNγ, anti-CXCR3 and anti-CXCR4 were purchased from BD Biosciences-Pharmingen. Anti-MHCII, anti-CD207, anti-Gr-1, anti-CD11b, and anti-Foxp3 staining kit were purchased from eBioscience. Murine recombinant IL-2, IL-6 and E-selection-FC were purchased from R&D Systems. BD Cytofix/Perm buffer was used for intracellular staining. Cells were run on a BD LSR II flow cytometer or a Beckman Coulter Gallios flow cytometer and analyzed by Flowjo (Flowjo LLC).
Zhang R., Borges C.M., Fan M.Y., Harris J.E, & Turka L.A. (2015). Requirement for CD28 in Effector Treg Differentiation, CCR6 induction, and Skin Homing. Journal of immunology (Baltimore, Md. : 1950), 195(9), 4154-4161.
+ Open protocol
+ Expand
8

T Cell Phenotyping of TBI-1301 Infusion Products

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
T cell phenotypes of the TBI-1301 products were analyzed using the following antibodies: anti-CD3 (Becton Dickinson Biosciences, Franklin Lakes, New Jersey, USA), anti-CD4 (Becton Dickinson Biosciences), anti-CD8 (Becton Dickinson Biosciences), anti-CD45RA (Beckman Coulter, Brea, California, USA), and anti-CCR7 (BioLegend, San Diego, California, USA). T cell differentiation status of the infusion products was determined by measuring the proportion of stem cell-like memory (CD45RA+/CCR7+), central memory (CD45RA−/CCR7+), effector memory (CD45RA−/CCR7−), and terminally differentiated (CD45RA+/CCR7−) T cells.22 (link) Data analysis was performed using FlowJo (Tree Star, San Carlos, California, USA).
Ishihara M., Kitano S., Kageyama S., Miyahara Y., Yamamoto N., Kato H., Mishima H., Hattori H., Funakoshi T., Kojima T., Sasada T., Sato E., Okamoto S., Tomura D., Nukaya I., Chono H., Mineno J., Kairi M.F., Diem Hoang Nguyen P., Simoni Y., Nardin A., Newell E., Fehlings M., Ikeda H., Watanabe T, & Shiku H. (2022). NY-ESO-1-specific redirected T cells with endogenous TCR knockdown mediate tumor response and cytokine release syndrome. Journal for Immunotherapy of Cancer, 10(6), e003811.
+ Open protocol
+ Expand
9

Comprehensive Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood sample collected from the patient. The cells were then used for flow cytometric analysis according to the manufacturer’s instructions (Mindray, Shenzhen, China), the relevant kits were used for flow cytometry to measure the surface phenotype of immune cells. Anti-CD3 was purchased from Beckman Coulter (Miami, FL, USA) or BD Biosciences (San Jose, CA, USA). Antibodies of CD45, IFN-γ, IL-4, CD28, CD38, CD45RA, CD45, CD4, CD25, and FoxP3 Alexa Fluor® 647 were provided by BD Pharmingen (San Diego, CA, USA). Antibodies of CD4, CD45, CD8, CD4, and CD3 were bought from BD Biosciences (San Jose, CA, USA). Anti-CD8, anti-CCR7, and anti-HLA-DR were from BioLegend (San Diego, CA, USA). Anti-IL-17 was provided by eBioscience (San Diego, CA, USA).
Ding X., Huang H., Zhong L., Chen M., Peng F., Zhang B., Cui X, & Yang X.A. (2021). Disseminated Talaromyces marneffei Infection in a Non-HIV Infant With a Homozygous Private Variant of RELB. Frontiers in Cellular and Infection Microbiology, 11, 605589.
+ Open protocol
+ Expand
10

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fluorescence staining was performed using the following antibodies: anti-CD11c (AF448 labeled), anti-CD11b (BV510 labeled) anti–I-Ab (BV421 labeled), anti-CCR7 (BV421 labeled), anti-CXCR4 (APC labeled), anti-PD-L1 (APC labeled), anti-PD-L2 (PE labeled) all from Biolegend (San Diego, CA, USA) and anti-CD40 (APC labeled) and anti-CD86 (PE labeled) from eBioscience (San Diego, CA, USA). Fluorescence was analyzed using a FACS Canto™ II (Becton Dickinson, Heidelberg, Germany) flow cytometer and Flowjo™ software (version 10.0.8, FlowJoLLC, Ashland, OR, USA).
Gaffal E., Kemter A.M., Scheu S., Leite Dantas R., Vogt J., Baune B., Tüting T., Zimmer A, & Alferink J. (2020). Cannabinoid Receptor 2 Modulates Maturation of Dendritic Cells and Their Capacity to Induce Hapten-Induced Contact Hypersensitivity. International Journal of Molecular Sciences, 21(2), 475.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!