The treated HUVECs (500 µL/well) were added into a 24-well plate coated with Matrigel matrix (BD Biosciences). After 4 h, calcein solution (Solarbio, Beijing, China) was added at 37℃ for 15–30 min. The cells were observed under a fluorescence microscope (Nikon TiS, Tokyo, Japan) with an excitation wavelength of 490 nm and an emission wavelength of 515 nm. The tube formation results were observed under an inverted microscope and analyzed using the Image J software.
Calcein solution
Manufactured by Solarbio
Sourced in China
Calcein solution is a fluorescent dye commonly used in biological research applications. It is a water-soluble, high-affinity calcium indicator that emits green fluorescence when bound to calcium ions. The core function of calcein solution is to act as a fluorescent marker for the detection and visualization of calcium-related processes in cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using calcein solution
1
Angiogenic Potential of HUVECs
2
Hydrogel Vascularization Potential Evaluation
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Cell migration was observed using Transwell (8 µm of pore size; Bilfil, China) to assess the recruitment of vascular endothelial cells by the hydrogel. After 24h of culture, cells migrating from the transwell chamber to the bottom were fixed and stained with 0.5% CV for 30 min. The vascularization capacity of the hydrogels was examined by seeding 2×10 4 HUVECs in a 24-well plate coated with Matrigel (BD Biosciences, NJ) and incubating them at 37°C for 12h. The cells were then stained with a calcein solution (Solarbio, China) for 15 min. Finally, images were captured using an EVOS scanning system (EVOS FL, Thermo-Fisher, USA), and semi-quantitative analysis of cell movement rates was performed in ImageJ.
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