Mir 101 inhibitor

The Jurkat and HEK293 cell lines were purchased from the American Type Culture Collection (ATCC; Rockville, MD, USA), and cultured in RPMI-1640 medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) at 37°C in a humidified atmosphere with 5% CO₂. Adriamycin (ADM) was obtained from Sangon Biotech Co., Ltd., (Shanghai, China) and dissolved in phosphate-buffered saline (PBS). Cells were treated with 5 µg/ml adriamycin for 24 h. The cells were transfected with miR-negative control (NC), miR-101 mimic, miR-101 inhibitor, Notch1-pcDNA3.1 or pcDNA3.1 empty vector (Shanghai GenePharma, Co., Ltd., Shanghai, China) using Lipofectamine 2000 (Invitrogen, Waltham, MA, USA) following the manufacturer's protocols. After 6 h, the medium was replaced with fresh medium for further experiments.