We fixed the mice, after exsanguination, in 4% paraformaldehyde by perfusion and isolated dorsal root ganglia (DRG) at L3–L5. Isolated thigh bones were decalcified in 10% EDTA for one week. DRG and thigh bones were cut into 10 μm-thick sections and incubated with anti-proinsulin (mouse monoclonal; Fitzgerald Industries International, Acton, MA, USA), anti-TNF-α antibody (goat polyclonal; Santa Cruz Biotechnology, Dallas, TX, USA) and anti-microtubule-associated protein 2 (MAP2) antibody (rabbit polyclonal; Cell Signaling Technology, Danvers, MA, USA). Next we incubated the sections with species-matched fluorescence-labelled second antibodies, and observed the sections under a fluorescence or light microscope (Zeiss, Thornwood, NY, USA; Nikon, Tokyo, Japan). We counted 350–500 DRG neurons per mouse in at least three sections separated by 50 μm intervals. The number of immunopositive cells was normalised to the total number of neurons counted.
Anti microtubule associated protein 2 antibody
Manufactured by Cell Signaling Technology
Sourced in United States
The Anti-microtubule-associated protein 2 (MAP2) antibody is a laboratory reagent used to detect and study the MAP2 protein, which is a key component of the cytoskeleton in neurons. MAP2 plays a crucial role in the stabilization and organization of microtubules within neuronal cells. This antibody can be used in various applications, such as immunohistochemistry, immunocytochemistry, and Western blotting, to visualize and analyze the distribution and expression of MAP2 in biological samples.
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Lab products found in correlation
Anti proinsulin, by Biosynth (1 mentions)
Anti tnf α antibody, by Santa Cruz Biotechnology (1 mentions)
Glutamate, by Thermo Fisher Scientific (1 mentions)
Glutamine, by Thermo Fisher Scientific (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Poly l lysine hydrobromide, by Merck Group (1 mentions)
Neurobasal medium, by Thermo Fisher Scientific (1 mentions)
Anti glial fibrillary acidic protein antibody, by Merck Group (1 mentions)
Chitosan from crab shells, by Merck Group (1 mentions)
B27 supplement, by Thermo Fisher Scientific (1 mentions)
Sodium alginate, by Merck Group (1 mentions)
2 protocols using anti microtubule associated protein 2 antibody
1
Proinsulin and Neuroinflammation in DRG
2
Neuronal and Glial Cell Culture Protocols
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Chitosan from crab shells, sodium alginate, polyethyleneimine (MW 60kD), poly‐l ‐lysine hydrobromide (MW15‐30kD), hydrated polyhydroxy small gap fullerenes, bovine serum albumin (BSA), 4′,6‐diamidino‐2‐phenylindole (DAPI) nuclear stain and (3‐4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) were purchased from Sigma‐Aldrich (St. Louis, MO, USA). Neurobasal medium, B27 supplement, glutamine, and glutamate were purchased from Life Technologies (Carlsbad, CA, USA). Anti‐microtubule‐associated protein 2 (MAP2) antibody was purchased from Cell Signaling Technology (Danvers, MA, USA), and anti‐glial fibrillary acidic protein (GFAP) antibody was purchased from Sigma‐Aldrich (St. Louis, MO, USA).
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