Sch23390

SST was purchased from Bachem. The following chemicals were products of R&D Systems: tetrodotoxin (TTX), kynurenic acid, picrotoxin, CGP55845, BAPTA, SCH23390, linopirdine, ZD7288, CH275, L-803,087, BIM 23052, 1R,1′S,3′R/1R,1′R,3′S)-L-054,264 (abbreviated as L-054,264), octreotide, CYN154806, GDP-β-S, MDL12330A, SQ22536, Rp-cAMPS, H89, KT5720, GF109203X, forskolin, IBMX, HJC0350. PKI(5-24) was provided by Enzo Life Sciences. Drugs were initially prepared in stock solution, aliquoted and stored at −20°C. For those chemicals which are only soluble in dimethyl sulfoxide (DMSO), the concentration of DMSO was less than 0.1%. This concentration of DMSO either in the recording pipettes or in the bath had no significant effects on neuronal activity.

Nicotine hydrogen tartrate salt was purchased from Sigma-Aldrich (St. Louis, MO) and mixed in sterile saline, the pH was adjusted to 7.0 (±0.2) with a dilute NaOH solution. Nicotine dose (0.4 mg/kg) was calculated from the freebase form and the solution was injected subcutaneously 15 min before testing sessions unless otherwise noted. SCH-23390, (−)-eticlopride hydrochloride, and flupenthixol dihydrochloride were purchased from R&D Systems (Minneapolis, MN) and mixed in sterile saline. All DA antagonists were injected into the intraperitoneal cavity (ip) 30 min before test sessions. Powdered Nesquick^® (chocolate) was purchased from a local market and dissolved in tap water at a concentration of 5% (w/v).

The following chemicals were products of R&D Systems: AVP, tetrodotoxin (TTX), kynurenic acid, picrotoxin, SR49059, TASP0390325, tolvaptan, GDP-β-S, U73122, U73343, heparin, thapsigargin, chelerythrine, bisindolylmaleimide II (Bis II), KB-R7943, capsazepine, AMG9810, AMG1629, capsaicin, ML 133, ML 297 and SCH23390. Dioctanoyl phosphatidylinositol 4,5-bisphosphate (dic8-PIP₂) was purchased from Echelon Biosciences. Drugs were initially prepared in stock solution, aliquoted and stored at −20°C. For those chemicals requiring dimethyl sulfoxide (DMSO) as a solvent, the concentration of DMSO was less than 0.1%. This concentration of DMSO either in the recording pipettes or in the bath had no significant effects on neuronal activity.