Phospho iκb ser32

Manufactured by Santa Cruz Biotechnology

Phospho-IκB (Ser32) is a laboratory reagent used to detect and quantify the phosphorylation of the serine 32 residue on the IκB protein. IκB is an important regulator of the NF-κB signaling pathway, and its phosphorylation at Ser32 is a key step in the activation of NF-κB. This product can be used in various biochemical and cell-based assays to study the regulation of the NF-κB pathway.

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Lab products found in correlation

Redd1, by Proteintech (1 mentions) Odyssey nitrocellulose membrane, by LI COR (1 mentions) Odyssey imager, by LI COR (1 mentions) Irdye secondary antibody, by LI COR (1 mentions) Fluocinolone acetonide, by Merck Group (1 mentions) Lamin b, by Cell Signaling Technology (1 mentions) Mk2206, by Merck Group (1 mentions) Wortmannin, by Merck Group (1 mentions) Fkbp51, by Santa Cruz Biotechnology (1 mentions) Gapdh g9545, by Merck Group (1 mentions) Nvp bez235, by Merck Group (1 mentions) Redd1 10638 1 ap, by Proteintech (1 mentions) Azd8055, by Merck Group (1 mentions) Phospho rps6, by Cell Signaling Technology (1 mentions) Phospho 4ebp1, by Cell Signaling Technology (1 mentions) Phospho p70s6k, by Cell Signaling Technology (1 mentions) 5 bromo 2 deoxyuridine brdu, by Merck Group (1 mentions)

2 protocols using phospho iκb ser32

Quantitative Western Blot Analysis

Cited 1 time

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Western blot analysis of whole-cell, cytoplasmic, and nuclear protein extracts was performed as described (Baida et al., 2015 (link)). Proteins were resolved by SDS-PAGE and transferred to Odyssey nitrocellulose membranes (LI-COR Biosciences, Lincoln, NE). After blocking, membranes were incubated with primary antibodies to GR (H-300 or M-20), RelA/p65, phospho-RelA/p65 (Ser536), IκB, and phospho-IκB (Ser32) (Santa Cruz Biotechnology, Dallas, TX); raptor, phospho-GR (Ser211), phospho-rpS6 (Ser235/236), phospho-4EBP1 (Thr37/46), pP70S6K (Thr389), lamin B, and tubulin (Cell Signaling, San Jose, CA); and REDD1 (Proteintech Group, Rosemont, IL), followed by IRDye secondary antibodies (LI-COR Biosciences), and signal was visualized by LI-COR Odyssey Imager. Results were normalized to GAPDH and tubulin expression for whole-cell proteins and to lamin B and HDAC1 for nuclear proteins.

Lesovaya E., Agarwal S., Readhead B., Vinokour E., Baida G., Bhalla P., Kirsanov K., Yakubovskaya M., Platanias L.C., Dudley J.T, & Budunova I. (2018). Rapamycin Modulates Glucocorticoid Receptor Function, Blocks Atrophogene REDD1, and Protects Skin from Steroid Atrophy. The Journal of investigative dermatology, 138(9), 1935-1944.

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Signaling Pathway Regulation in Cells

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Fluocinolone acetonide (FA), croton oil (CO), Wortmannin (WM), AZD8055 (AZD), NVP-BEZ235 (NVP), MK-2206 (MK), and 5-bromo-2′-deoxyuridine (BrdU), were from Sigma Aldrich (St. Louis, MO), LY294002 (LY) was from LC Laboratories (Woburn, MA). TNF-α and cytoplasmic and nuclear fractionation kit were from Thermo Fisher Scientific (Waltham, MA).
We used antibodies to GR (sc-8992, RRID:AB_2155784), RelA/p65 (sc-8008, RRID:AB_628017), phospho-RelA/p65 (Ser536) (sc-136548, RRID:AB_10610391), IκB (sc-1643, RRID:AB_627772), phospho-IκB (Ser32) (sc-8404, RRID:AB_627773), FKBP51 (sc-271547, RRID:AB_10649040) (Santa Cruz Biotechnology, Dallas, TX); GAPDH (G9545, RRID:AB_796208, Sigma Aldrich); phospho-GR (Ser211) (4161, RRID:AB_2155797), phospho-rpS6 (Ser235/236) (4856S, RRID:AB_2181037), phospho-4E-BP1 (Thr37/46) (9459L, RRID:AB_2262165), phospho-P70S6K (Thr389) (9234, RRID:AB_2269803), phospho-AKT (Thr308 and Ser473) (9266S, RRID:AB_659801 and 9271, RRID:AB_329825), lamin B (12586, RRID:AB_2650517) and tubulin (2148, RRID:AB_2288042) (Cell Signaling, San Jose, CA), REDD1 (10638-1-AP, RRID,AB_2245711, Proteintech Group, Rosemont, IL).

Agarwal S., Mirzoeva S., Readhead B., Dudley J.T, & Budunova I. (2019). PI3K inhibitors protect against glucocorticoid-induced skin atrophy. EBioMedicine, 41, 526-537.

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