Methylcellulose

Manufactured by Nacalai Tesque

Sourced in Japan

Methylcellulose is a water-soluble cellulose derivative used in various laboratory applications. It functions as a thickening, stabilizing, and binding agent.

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Lab products found in correlation

Penicillin streptomycin, by Nacalai Tesque (1 mentions) Glasgow minimum essential medium, by Merck Group (1 mentions) Non essential amino acids (neaa), by Nacalai Tesque (1 mentions) Sodium pyruvate, by Nacalai Tesque (1 mentions) β mercaptoethanol, by Nacalai Tesque (1 mentions) Calcein am, by Dojindo Laboratories (1 mentions) Hybrid cell count module, by Keyence (1 mentions) Olmesartan, by Daiichi Sankyo (1 mentions) Bz x710 fluorescence microscope, by Keyence (1 mentions) Sp8 confocal microscope, by Leica (1 mentions)

Close spelling variants of this product

Methylcellulose #400 (2 citations)

6 protocols using methylcellulose

Mouse ESC Differentiation via Embryoid Body

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The mouse ESC lines EB5 and ZHBTc4 were kindly provided by Dr Hitoshi Niwa (22 (link),23 (link)). The cells were cultured in Glasgow minimum essential medium (Sigma-Aldrich) containing 10% fetal bovine serum, 1000 U/ml recombinant leukemia inhibitory factor (LIF) (Nacalai Tesque Inc.), 1 mM sodium pyruvate (Nacalai Tesque Inc.), 0.1 mM non-essential amino acids (Nacalai Tesque Inc.), 0.1 mM β-mercaptoethanol (Nacalai Tesque Inc.) and penicillin/streptomycin (Nacalai Tesque Inc.). They were cultured on gelatin-coated plates at 37°C in a 5% CO₂ incubator.
Differentiation of ESCs was performed by the induction of embryoid body (EB) formation in vitro. ESCs were suspended in medium without LIF and drops of 400 cells/20 μl were transferred to the lid of a bacterial grade Petri dish (hanging drop method) (24 (link)). These hanging drops were incubated for 2 days and subsequently 50 EBs were transferred to differentiation medium with 2% methylcellulose (Nacalai Tesque Inc.) in each well of 24-well culture plates. The EBs were cultured further and collected at 4 days after the withdrawal of LIF.

Semba Y., Harada A., Maehara K., Oki S., Meno C., Ueda J., Yamagata K., Suzuki A., Onimaru M., Nogami J., Okada S., Akashi K, & Ohkawa Y. (2017). Chd2 regulates chromatin for proper gene expression toward differentiation in mouse embryonic stem cells. Nucleic Acids Research, 45(15), 8758-8772.

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Glioblastoma Spheroid Assay Protocol

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T98G and A172 cells were cultured in spheroid culture medium containing 1.6% (w/v) methylcellulose (Nacalai Tesque). Fresh aliquots of MACS NeuroBrew‐21, EGF and bFGF were added at day 3. After 6 days in culture, the living cells were stained with Calcein‐AM (Dojindo) and counted using hybrid cell count module (Keyence). In some experiments, cells were treated with Strictinin (Nagara Science Co., Ltd.), a Ror1 inhibitor. The numbers and sizes of spheroids formed were measured, and spheroids were classified into small (<1000 μm²), medium (1001–2000 μm²), and large (>2001 μm²) according to their sizes.

Ishikawa T., Ogura Y., Tanaka K., Nagashima H., Sasayama T., Endo M, & Minami Y. (2022). Ror1 is expressed inducibly by Notch and hypoxia signaling and regulates stem cell‐like property of glioblastoma cells. Cancer Science, 114(2), 561-573.

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Dietary Salt-Induced Kidney Damage

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At 6 weeks of age, DSS rats were selected at random to receive rat chow containing normal salt (NS: 0.5% NaCl, Oriental Yeast, Tokyo, Japan) or high salt (HS: 4% NaCl, Oriental Yeast) for 14 weeks from 6 to 20 weeks of age. From 6 to 20 weeks of age, HS-fed DSS rats were further treated with either vehicle (0.5% methyl cellulose, Nacalai Tesque, Kyoto, Japan), olmesartan (OLM: oral gavage; 10 mg/kg body weight/day, p.o.; Daiichi-Sankyo Co., Ltd., Tokyo, Japan), azelnidipine (AZEL: 3 mg/kg body weight/day, p.o.; Daiichi-Sankyo Co., Ltd.), their combination, or hydralazine (HYD: 50 mg/kg body weight/day, p.o.; Wako Co., Ltd., Osaka, Japan). The doses of OLM and AZEL were chosen on the basis of results from previous rat studies [12] (link), [20] (link). Preliminary studies also showed that HYD at 50 mg/kg substantially decreased blood pressure in HS-treated DSS rats (data not shown). Kidneys were perfused with saline and harvested under sodium pentobarbital anesthesia (65 mg/kg) at 10, 15 and 20 weeks of age (n = 7 for each). All data from Protocol-1 are shown as in Supplemental Figures.

Rafiq K., Nishiyama A., Konishi Y., Morikawa T., Kitabayashi C., Kohno M., Masaki T., Mori H., Kobori H, & Imanishi M. (2014). Regression of Glomerular and Tubulointerstitial Injuries by Dietary Salt Reduction with Combination Therapy of Angiotensin II Receptor Blocker and Calcium Channel Blocker in Dahl Salt-Sensitive Rats. PLoS ONE, 9(9), e107853.

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Visualizing Embryonic Cell Development

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To visualize embryonic cells, 10 kDa Alexa 488-dex (total 10 ng) was injected into two blastomeres at the 2-cell stage. At stage 13–14, archenteron fluid was labeled by injecting 10 kDa Alexa 568-dex (2 nL, 10 mg/mL) into the archenteron. A 5 μm-thick, ∼100 μm-wide strip of aluminum foil (AL-013131; Nilaco, Tokyo, Japan) coated with 0.1% BSA was inserted into the blastopore of stage 14 embryos with the vitelline membrane removed. To confirm proper insertion, embryos were fixed with 2% TCA and 9.25% formaldehyde and then cleared with benzyl alcohol/benzyl benzoate (BABB) solution. Fluorescence images were acquired using an SP8 confocal microscope with a 10× objective (Leica).
Time-lapse images were acquired using a BZ-X710 fluorescence microscope (Keyence) with a 2× objective (Nikon). To prevent flow of culture medium, high-viscosity 0.1x Barth’s medium containing 3% methyl cellulose (#1500; Nacalai Tesque, Kyoto, Japan) was used.

Kato S, & Inomata H. (2023). Blastopore gating mechanism to regulate extracellular fluid excretion. iScience, 26(5), 106585.

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Screening Erythropoiesis Candidate Genes

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The process of screening candidate genes involved in erythropoiesis was performed as previously described [2] . In brief, 8 candidate genes with full-length insertions in transduced cells were selected from our previous report. cDNA from each gene was cloned into the pCSII-EF-RfA-IRES2-Venus lentiviral vector (kindly provided by H. Miyoshi, RIKEN, Tsukuba, Japan) using Gateway Clonase Enzyme Mix (Invitrogen, Carlsbad, CA). All constructs were verified by DNA sequencing. Specific lentiviral supernatant was produced from 293T cells and used to transduce UT-7/Epo cells. Cells transduced with each of the 8 lentiviruses were cultured in methylcellulose (Nacalai Tesque, Kyoto, Japan) without Epo for 1 month before analysis.

Kummalue T., Inoue T., Miura Y., Narusawa M., Inoue H., Komatsu N., Wanachiwanawin W., Sugiyama D, & Tani K. (2015). Ribosomal protein L11- and retinol dehydrogenase 11-induced erythroid proliferation without erythropoietin in UT-7/Epo erythroleukemic cells. Experimental hematology, 43(5).

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Effect of SGLT2 Inhibitor on Blood Pressure in Rats

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Rats were maintained in a temperature-controlled (24 ± 2 °C) room with a 12-h light/dark cycle and 55 ± 5% humidity. Experimental protocols and animal care were performed according to the guidelines for the care and use of animals established by Kagawa University, Japan.
Experiments were performed in 21 male 10-week-old OLETF rats and 8 age-matched lean Long-Evans Tokushima Otsuka (LETO) rats (Hoshino Laboratory Animals, Sakai-Higashi, Japan). 22 At 13 weeks of age, the radiotelemetry device was implanted under isoflurane anesthesia. After 2 weeks of acclimatization, all animals were treated with 1% NaCl in drinking water and normal salt diet (0.5% NaCl, SLC Japan, Hamamatsu, Japan) from 15 weeks of age. LETO rats were also treated with vehicle (0.5% methyl cellulose; Nacalai Tesque, Kyoto, Japan), while OLETF rats were randomly divided into two groups for treatment with vehicle (n = 10) or the selective SGLT2 inhibitor, empagliflozin (10 mg kg -1 per day; p.o., n = 11). The dose of empagliflozin was determined on the basis of previous studies in rats. 23, 24 Animals underwent treatment for 5 weeks. Mean arterial pressure (MAP) was measured in conscious rats by the telemetry device. 17 Twenty-four-hour urine samples were also collected every 2-3 weeks using metabolic cages.

Takeshige Y., Fujisawa Y., Rahman A., Kittikulsuth W., Nakano D., Mori H., Masaki T., Ohmori K., Kohno M., Ogata H, & Nishiyama A. (2016). A sodium-glucose co-transporter 2 inhibitor empagliflozin prevents abnormality of circadian rhythm of blood pressure in salt-treated obese rats. Hypertension research : official journal of the Japanese Society of Hypertension, 39(6).

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