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Gant58

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GANT58 is a small molecule inhibitor that targets the enzyme GANT58. It is commonly used in research applications to study the role of GANT58 in various biological processes.

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Lab products found in correlation

Cyclopamine, by Bio-Techne (1 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Rtgfβ1, by R&D Systems (1 mentions) Sb431542, by R&D Systems (1 mentions) Tubulin, by Merck Group (1 mentions) Rad001, by Selleck Chemicals (1 mentions) Prmt1, by Cell Signaling Technology (1 mentions) Gdc 0449, by Selleck Chemicals (1 mentions) Nvp lde225, by Selleck Chemicals (1 mentions) Ami 1, by Merck Group (1 mentions) Sybr green real time pcr kit, by Bio-Rad (1 mentions) Gant61, by Bio-Techne (1 mentions) Flag m2 magnetic beads, by Merck Group (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Tgf β, by Thermo Fisher Scientific (1 mentions) Actin, by Merck Group (1 mentions)

3 protocols using gant58

1

Chondrocyte signaling pathway regulation

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Antler chondrocytes were isolated by enzymatic digestion as previously described20 and cultured with DMEM‐high glucose (Hyclone) supplemented with 10% foetal bovine serum (Life Technologies). These cultured chondrocytes were treated with recombinant human/mouse/rat TGFβ1 protein (rTGFβ1, 100 ng/mL; R&D Systems) in the absence or presence of TGFβ receptor inhibitor SB431542 (10 µmol/L, MCE), Smad3 inhibitor SIS3 (10 µmol/L; Selleck), Notch signalling inhibitor DAPT (25 µmol/L; Sigma), Smo antagonist cyclopamine (10 µmol/L; Tocris Bioscience) and Gli1 antagonist GANT58 (10 µmol/L; Tocris Bioscience), respectively. Additionally, chondrocytes were incubated with SB431542 or SIS3 and then supplemented recombinant human Shh protein (rShh, 25 ng/mL; R&D Systems).
Ma L., Yang Z., Ding J., Liu S., Guo B, & Yue Z. (2019). Function and regulation of transforming growth factor β1 signalling in antler chondrocyte proliferation and differentiation. Cell Proliferation, 52(4), e12637.
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2

Hedgehog Signaling Pathway Assay

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Recombinant hedgehog (Shh) was purchased from R&D System (Minneapolis, MN), while Gant 58 was purchased from Tocris Bioscience (Ballwin, MO).
Lan X., Wen H., Cheng K., Plagov A., Shoshtari S.S., Malhotra A, & Singhal P.C. (2017). Hedgehog pathway plays a vital role in HIV-induced epithelial-mesenchymal transition of podocyte. Experimental cell research, 352(2), 193-201.
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3

Antibody Specifications and Peptide Details for Methylated Gli1 Analysis

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The antibodies used in this study were Gli1 (#3538, Cell Signaling Technology, for western blotting and chromatin immunoprecipitation (ChIP) assay, and#sc-20687, Santa Cruz Biotechnology, for immunohistochemistry), actin (#A2066, Sigma-Aldrich), Flag (#F3165, Sigma-Aldrich), Flag M2 magnetic beads (#M8823, Sigma-Aldrich), PRMT1 (#2449, Cell Signaling Technology), and tubulin (#T5168, Sigma-Aldrich). GDC-0449, NVP-LDE225, and RAD-001 were purchased from Selleck Chemicals LLC, GANT58 and GANT61 from Tocris Bioscience, and AMI-1 from Sigma-Aldrich. The SYBR Green real-time PCR kit was obtained from Bio-Rad and TGF-β from Peprotech (#100-21). The antibody against R597-methylated Gli1 was developed using the following synthetic peptide with asymmetric dimethylation at R597 as antigen: RARYASA-[R597(aMe2)]-GGGTS (C-terminal amidation and N-terminal KLH conjugation). Other peptides used in the study included the following:

Cold Gli1 peptide without R597 methylation [Gli1-R597]: RARYASA-[R597]-GGGTS;

Hot Gli1 peptide with R597 monomethylation [Gli1-R597(Me)]: RARYASA-[R597(Me)] -GGGTS;

Hot Gli1 peptide with R597 asymmetric dimethylation [Gli1-R597(aMe2)]: RARYASA-[R597(aMe2)]-GGGTS;

Hot Gli1 peptide with R597 symmetric dimethylation [Gli1-R597(sMe2)]: RARYASA-[R597(sMe2)]-GGGTS.

Wang Y., Hsu J.M., Kang Y., Wei Y., Lee P.C., Chang S.J., Hsu Y.H., Hsu J.L., Wang H.L., Chang W.C., Li C.W., Liao H.W., Chang S.S., Xia W., Ko H.W., Chou C.K., Fleming J.B., Wang H., Hwang R.F., Chen Y., Qin J, & Hung M.C. (2016). Oncogenic functions of Gli in pancreatic adenocarcinoma are supported by its PRMT1-mediated methylation. Cancer research, 76(23), 7049-7058.
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