Total RNA was extracted using either Qiagen miRNeasy or the RNeasy Plus mini kits and quantified by a nanodrop spectrophotometer (Thermo Sci.). For detection of mature miRNAs, the TaqMan® MicroRNA assay kit (Applied Biosystems) was used according to the manufacturer’s instructions. Mature miR-21 levels were assayed using RNU48 as internal control for normalization. Data are presented as fold changes in miR-21 levels (2-ΔΔCt). Relative expression of SMA and PDCD4 mRNA was normalized to GAPDH. The following primers were used: GAPDH 5′-ACCACAGTCCATGCCATCAC-3′ (forward) and 5′-TCCACCACCCTGTTGCTGTA-3′ (reverse); SMA: 5′-CCAGCTATGTGTGAAGAAGAGG-3′ (forward) and 5′-GTGATCTCCTTCTGCATTCGGT-3′ (reverse). PDCD4: 5′-TATGATGTGGAGGAGGTGGATGTGA-3′ (reverse) and 5′-TATGATGTGGAGGAGGTGGATGTGA-3′ (reverse). Statistical analysis was performed by ANOVA followed by Bonferroni’s multiple comparisons test. An average of three experiments, each performed in triplicate with standard errors, is presented. The TaqMan® probes for mature miR-21-5p (Cat. # 4427975, ID 000397) and RNU48 (human) and TaqMan® Control miRNA Assay were purchased from Life Technologies.
Taqman control mirna assay
Manufactured by Thermo Fisher Scientific
The TaqMan® Control miRNA Assay is a laboratory instrument used for the detection and quantification of microRNA (miRNA) expression levels. It provides a standardized and consistent method for measuring the abundance of specific miRNA molecules within a sample. The assay utilizes TaqMan® technology, a real-time PCR-based approach, to accurately measure miRNA levels.
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Lab products found in correlation
2 protocols using taqman control mirna assay
1
miRNA-21 Expression Analysis in Cells
2
miRNA-21 Expression Analysis in Cells
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Total RNA was extracted using either Qiagen miRNeasy or the RNeasy Plus mini kits and quantified by a nanodrop spectrophotometer (Thermo Sci.). For detection of mature miRNAs, the TaqMan® MicroRNA assay kit (Applied Biosystems) was used according to the manufacturer’s instructions. Mature miR-21 levels were assayed using RNU48 as internal control for normalization. Data are presented as fold changes in miR-21 levels (2-ΔΔCt). Relative expression of SMA and PDCD4 mRNA was normalized to GAPDH. The following primers were used: GAPDH 5′-ACCACAGTCCATGCCATCAC-3′ (forward) and 5′-TCCACCACCCTGTTGCTGTA-3′ (reverse); SMA: 5′-CCAGCTATGTGTGAAGAAGAGG-3′ (forward) and 5′-GTGATCTCCTTCTGCATTCGGT-3′ (reverse). PDCD4: 5′-TATGATGTGGAGGAGGTGGATGTGA-3′ (reverse) and 5′-TATGATGTGGAGGAGGTGGATGTGA-3′ (reverse). Statistical analysis was performed by ANOVA followed by Bonferroni’s multiple comparisons test. An average of three experiments, each performed in triplicate with standard errors, is presented. The TaqMan® probes for mature miR-21-5p (Cat. # 4427975, ID 000397) and RNU48 (human) and TaqMan® Control miRNA Assay were purchased from Life Technologies.
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