E500 580 epr spectrometer

Manufactured by Bruker

The E500/580 EPR spectrometer is a high-performance electron paramagnetic resonance (EPR) spectrometer designed for advanced research applications. It offers a versatile platform for the detection and analysis of paramagnetic species, providing researchers with the necessary tools to investigate a wide range of materials and samples. The spectrometer's core function is to generate a strong magnetic field and microwave radiation to induce and measure the resonant absorption of electromagnetic energy by unpaired electrons within the sample, enabling the identification and characterization of these paramagnetic species.

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Lab products found in correlation

Super high q cavity er 4122shqe, by Oxford Instruments (2 mentions) Esr900 helium, by Oxford Instruments (1 mentions) 4 mm quartz tubes, by Wilmad (1 mentions) Esr900 helium flow cryostat, by Oxford Instruments (1 mentions)

2 protocols using e500 580 epr spectrometer

X-band EPR and ENDOR Spectroscopy

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EPR and ENDOR spectra were obtained using a Bruker E500/580 EPR spectrometer. Continuous wave X-band EPR spectra employed a Bruker “Super High Q” cavity (ER 4122SHQE) coupled to an Oxford Instruments ESR900 helium flow cryostat for temperature control. Spectra were acquired at 20 K using 10-microwatt microwave power, 100-kHz field modulation frequency, and 1 G modulation amplitude. X-band FID-detected Davies pulsed ENDOR spectra were collected at 30 K and g = 2.0033 using a Bruker EN 4118X-MD4 dielectric ENDOR resonator coupled to an ER 4118HV-CF100 Cryo-Free cooling system. The length of the initial inversion pulse was 400 ns, the detection pulse was 200 ns, and the radiofrequency pulse was 9 μs.

Bailey S.S., Payne K.A., Fisher K., Marshall S.A., Cliff M.J., Spiess R., Parker D.A., Rigby S.E, & Leys D. (2017). The role of conserved residues in Fdc decarboxylase in prenylated flavin mononucleotide oxidative maturation, cofactor isomerization, and catalysis. The Journal of Biological Chemistry, 293(7), 2272-2287.

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EPR Spectroscopy of Cryogenic Samples

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EPR spectra were obtained using a Bruker E500/580 EPR spectrometer. Continuous wave X-band (~9.4 GHz) EPR spectra employed a Bruker “Super High Q” cavity (ER 4122SHQE) coupled to an Oxford Instruments ESR900 helium flow cryostat for temperature control. Spectra were acquired at 20 K using 10-microwatt microwave power, 100-kHz field modulation frequency, and 1 -G modulation amplitude. Samples were stored as 300 µL aliquots in 4 -mm quartz tubes (Wilmad) prior to recording spectra.

Marshall S.A., Payne K.A., Fisher K., White M.D., Ní Cheallaigh A., Balaikaite A., Rigby S.E, & Leys D. (2019). The UbiX flavin prenyltransferase reaction mechanism resembles class I terpene cyclase chemistry. Nature Communications, 10, 2357.

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