Polyclonal rabbit anti hsv 1 antibody

Manufactured by Agilent Technologies

Sourced in United States, Denmark

The Polyclonal rabbit anti-HSV-1 antibody is a laboratory reagent produced by immunizing rabbits with Herpes Simplex Virus type 1 (HSV-1) antigens. This antibody recognizes multiple epitopes on various HSV-1 proteins, enabling the detection of the virus in samples.

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Lab products found in correlation

Dmem low glucose, by Merck Group (2 mentions) Horseradish peroxidase conjugated secondary anti igg antibodies, by Merck Group (2 mentions) Alexa fluor 555 secondary antibody, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Sodium selenite, by Merck Group (1 mentions) Putrescine, by Merck Group (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Dibutyryl cyclic amp dbcamp, by Merck Group (1 mentions) Carboxymethylcellulose sodium salt cmc, by Merck Group (1 mentions) Alexa 488 and alexa 647 conjugated secondary antibodies, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Anti-HSV-1 (4 citations) HSV-1 (3 citations) Anti-HSV-1 antibody (2 citations) Rabbit polyclonal anti-HSV antibody (1 citations) Anti-TM (1 citations)

6 protocols using polyclonal rabbit anti hsv 1 antibody

Dynasore-mediated Endocytosis Inhibition

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Dynasore (D7693), chlorpromazine (C8138), nystatin (N6261), o-nitrophenyl-β-D-galactopyranoside (ONPG), low-glucose DMEM and FBS were purchased from Sigma Chemical Co. (St. Louis, MO, USA).
Rabbit anti-dynamin 2 polyclonal antibody and peroxidase-conjugated anti-rabbit immunoglobulin G (IgG) were from Invitrogen (CA, USA), and rabbit anti-HSV-1 polyclonal antibody was from Dako (CA, USA). Alexa Fluor 555 secondary antibody was from Molecular Probes (Eugene, OR, USA). To-Pro-3 was from ThermoFisher (MA, USA). Mowiol was from Calbiochem (Merck Chemicals, Darmstadt, Germany), and human transferrin (Trf) CF^®543 and cholera toxin subunit B (CT-B) CF^®543 conjugates were purchased from Biotum (San Francisco, CA, USA).

Praena B., Bello-Morales R, & López-Guerrero J.A. (2020). Hsv-1 Endocytic Entry into a Human Oligodendrocytic Cell Line Is Mediated by Clathrin and Dynamin but Not Caveolin. Viruses, 12(7), 734.

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Immunofluorescence Assay for HSV-1 Detection

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Immunofluorescence assay (IFA) was done as described earlier.[12 (link)] Rabbit anti HSV-1 polyclonal antibody (DAKO, Denmark) was used as primary antibody on fixed smears. A positive control of Vero cell line infected with HSV 1 was used for each batch of test.

Guda S.J., Sontam B., Bagga B., Ranjith K., Sharma S, & Joseph J. (2019). Evaluation of multiplex real-time polymerase chain reaction for the detection of herpes simplex virus-1 and 2 and varicella-zoster virus in corneal cells from normal subjects and patients with keratitis in India. Indian Journal of Ophthalmology, 67(7), 1040-1046.

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Immunohistochemical Analysis of HSV-1 Infection in Corneal Tissue

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Following 1 and 8 days of culture, corneas were harvested and portions of the tissue processed for immunohistochemical staining. Briefly, non-fixed tissue was embedded in OCT media, frozen in liquid nitrogen, and cut into sagittal section (20 μm thick) using a cryostat. Frozen sections were placed on slides, permeated with 1% Triton X-100 in 1X PBS, and blocked 1 hour with 10% donkey serum (Jackson Immuno Labs, West Grove, PA) in 1X PBS, followed by incubation with polyclonal rabbit anti-HSV1 antibody diluted 1:100 (Dako, Carpinteria, CA) in blocking solution. Following three washes using 1X PBS, the tissue was incubated with FITC-conjugated donkey anti-rabbit antibody (Jackson Immuno Labs) at a 1:100 dilution for 1 hour. Nuclei were counterstained with the dye DAPI, and following washes with 1X PBS, samples were mounted with 50% glycerol/1X PBS, and analyzed using Olympus confocal laser scanning microscope (FV500, version 5.0, Olympus, Center Valley, PA).

Drevets P., Chucair-Elliott A., Shrestha P., Jinkins J., Karamichos D, & Carr D.J. (2015). The Use of Human Cornea Organotypic Cultures to Study Herpes Simplex Virus Type 1 (HSV-1) Induced Inflammation. Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie, 253(10), 1721-1728.

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Antibody-mediated cell signaling analysis

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Anti nectin-1 monoclonal antibody CK41 and anti-HVEM polyclonal antibody R140 have been described previously [46] (link), [47] (link). Horseradish peroxidase-conjugated secondary anti-IgG antibodies were purchased from Millipore (Billerica, MA, USA). Anti-green fluorescent protein GFP rabbit polyclonal serum A6455, Alexa 488-, Alexa 647- and Alexa 594-conjugated secondary antibodies were obtained from Molecular Probes (Eugene, OR, USA). DNA size marker was from Invitrogen. Polyclonal rabbit anti-HSV-1 antibody was from DAKO. Monoclonal mouse anti-PLP MAB388 antibody was from Millipore. Anti-nectin-1 mouse monoclonal antibody CK6 was from Santa Cruz Biotechnology. Anti-HVEM mouse monoclonal antibody, low-glucose DMEM, fetal bovine serum (FBS), human insulin, triiodothyronine (T3), apo-transferrin, sodium selenite, putrescine, dibutyryl cyclic AMP (dbcAMP), carboxymethylcellulose sodium salt (CMC) medium-viscosity and protease inhibitor cocktail were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Mowiol was from Calbiochem (Merck Chemicals, Germany). HS4C3 antibody was a kind gift of Dr. R. Longnecker, (Northwestern Medical School, Chicago, USA).

Bello-Morales R., Crespillo A.J., García B., Dorado L.Á., Martín B., Tabarés E., Krummenacher C., de Castro F, & López-Guerrero J.A. (2014). The Effect of Cellular Differentiation on HSV-1 Infection of Oligodendrocytic Cells. PLoS ONE, 9(2), e89141.

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Fluorescent Labeling of Anti-HSV-1 Antibody

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Polyclonal rabbit anti-HSV-1 antibody and HRP-conjugated anti-HSV-1 antibody were purchased from Dako. The unlabeled antibody was fluorescently labeled using the Alexa Flour 488 Labeling Kit from Thermo Fisher (catalog number A20181).

Masci A.L., Menesale E.B., Chen W.C., Co C., Lu X, & Bergelson S. (2019). Integration of Fluorescence Detection and Image-Based Automated Counting Increases Speed, Sensitivity, and Robustness of Plaque Assays. Molecular Therapy. Methods & Clinical Development, 14, 270-274.

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Immunofluorescence Labeling Protocol

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Horseradish peroxidase-conjugated secondary anti-IgG antibodies were purchased from Millipore (Billerica, MA, USA). Alexa 488- and Alexa 647-conjugated secondary antibodies were obtained from Molecular Probes (Eugene, OR, USA). Polyclonal rabbit anti-HSV-1 antibody was from Dako. Living Colors anti-reef coral fluorescent protein (RCFP) polyclonal pan antibody was from Clontech. Anti-LAMP-1 mouse monoclonal antibody H4A3 was from DSHB (Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, Iowa, USA). Sheep anti-TGN46 polyclonal antibody was from Serotec. Low-glucose Dulbecco modified Eagle (DMEM), fetal bovine serum (FBS), pooled human serum, carboxymethylcellulose sodium salt (CMC), and medium-viscosity and protease inhibitor cocktail were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Mowiol was from Calbiochem (Merck Chemicals, Germany).

López-Guerrero J.A., de la Nuez C., Praena B., Sánchez-León E., Krummenacher C, & Bello-Morales R. (2020). Herpes Simplex Virus 1 Spread in Oligodendrocytic Cells Is Highly Dependent on MAL Proteolipid. Journal of Virology, 94(4), e01739-19.

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