De14 801f

Manufactured by Lonza

Sourced in Belgium

The DE14-801F is a laboratory equipment product. It is designed to perform specific functions in a laboratory setting. No further details are available without making interpretations or extrapolations beyond the core purpose of the product.

Automatically generated - may contain errors

Lab products found in correlation

Sodium pyruvate, by Thermo Fisher Scientific (1 mentions) Knockout dmem, by Thermo Fisher Scientific (1 mentions) Esg1107, by Merck Group (1 mentions) 2 mercaptoethanol, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Chir99021, by Axon Medchem (1 mentions) Pd0325901, by Axon Medchem (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Cls 2, by Worthington (1 mentions) Be17 605e, by Lonza (1 mentions) Dmem culture medium, by Lonza (1 mentions)

3 protocols using de14 801f

Generating mESCs from WT and Morc3 Mutant Embryos

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mESCs were generated from WT and Morc3^MD41/MD41 preimplantation embryos and were cultured on 0.1% gelatin without feeders in mESC medium [Knockout DMEM (10829-018; Gibco), 10% FBS (DE14-801F; BioWhittaker), NEAA (11140; Gibco), l-Glutamine (25030-123; Gibco), Sodium Pyruvate (11360; Gibco), 2-Mercaptoethanol (31350; Gibco) and Leukemia Inhibitory Factor (ESG1107; Millipore)] plus MEK inhibitor PD0325901 (1 mM) and GSK3 inhibitor CHIR99021 (3 mM, Axon Medchem). Cell cultures tested negative for mycoplasma on a regular basis.

Desai V.P., Chouaref J., Wu H., Pastor W.A., Kan R.L., Oey H.M., Li Z., Ho J., Vonk K.K., San Leon Granado D., Christopher M.A., Clark A.T., Jacobsen S.E, & Daxinger L. (2021). The role of MORC3 in silencing transposable elements in mouse embryonic stem cells. Epigenetics & Chromatin, 14, 49.

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Chondrocyte Isolation and Expansion

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Cartilage was digested overnight in Dulbecco’s modified Eagle Medium (DMEM; 42430, Invitrogen) containing 0.1% w/v collagenase type II (CLS2, Worthington), 1% v/v penicillin/streptomycin (P/S; 15140, Invitrogen). Isolated chondrocytes were washed in PBS and expanded at 5000 cells/cm² in DMEM containing 1% P/S, 10% v/v fetal bovine serum (DE14-801F, Lonza), 10 ng/mL FGF (223-FB, R&D Systems). At 80% confluency, cells were trypsinized and passaged. Passage two cells were used for redifferentiation culture or snap-frozen for RNA analysis.

Rutgers M., Bach F., Vonk L., van Rijen M., Akrum V., van Boxtel A., Dhert W, & Creemers L. (2019). PTH decreases in vitro human cartilage regeneration without affecting hypertrophic differentiation. PLoS ONE, 14(4), e0213483.

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Culturing Human Cancer Cell Lines

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Human cancer cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), the European Collection of Cell Culture (ECACC, Salisbury, UK) and the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ, Braunschweig, Germany). Human cervical adenocarcinoma HeLa cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS). Human mammary carcinoma MCF-7 cells were cultured in RPMI supplemented with 10% FBS. The U87 and U373 cells were cultured in DMEM culture medium (Lonza code 12-136F, Vervier, Belgium), while the SKMEL-28 and A549 cells were cultured in RPMI culture medium (Lonza; code 12-115F) supplemented with 10% heat-inactivated FBS (Lonza, FBS South America code DE14-801F). Cell culture media were supplemented with 4 mM glutamine (Lonza code BE17-605E), 100 μg/mL gentamicin (Lonza code 17-5182), and penicillin-streptomycin (200 units/ml and 200 μg/ml) (Lonza code 17-602E). All cell lines were cultured in T25 flasks, maintained and grown at 37° C, 95% humidity, 5% CO₂.

Scott R., Karki M., Reisenauer M.R., Rodrigues R., Dasari R., Smith W.R., Pelly S.C., van Otterlo W.A., Shuster C.B., Rogelj S., Magedov I.V., Frolova L.V, & Kornienko A. (2014). Synthetic and Biological Studies of Tubulin Targeting C2-Substituted 7-Deazahypoxanthines Derived from Marine Alkaloid Rigidins. ChemMedChem, 9(7), 1428-1435.

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