Anti epcam pe

Manufactured by Thermo Fisher Scientific

Anti-EpCAM-PE is a fluorescently labeled antibody that binds to the Epithelial Cell Adhesion Molecule (EpCAM) protein. It is used for the detection and analysis of EpCAM-expressing cells in various applications, such as flow cytometry and cell sorting.

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EpCAM (56 citations) Anti-EpCAM (12 citations) Epcam-PE (6 citations) Anti-human EpCAM-PE antibody clone 1B7 (3 citations) Anti-mouse EpCAM-PE (2 citations) PE-conjugated anti-human EpCAM (2 citations) Anti-mouse CD326 (EpCAM) PE-Cy7 (2 citations) Anti-mouse CD326 (EpCAM)-PE antibody (clone: G8.8, (2 citations) Anti-human EpCAM-PE (2 citations) Anti-mouse PE-conjugated CD326 EpCAM (2 citations)

3 protocols using anti epcam pe

Single-Cell RNA-Seq of Skin Immune Cells

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Wil-type FVB mice were treated with 10 μg of anti-Skint1 or rat IgG2a isotype control by i.d. injection into the ear pinna, as described above. Seven days later, epidermal suspensions were made from ear skin and were stained as described above using Live/Dead Fixable Near-IR dye (Molecular Probes), anti-EpCAM-PE (eBioscience) and anti-CD45-APC and anti-I-A/I-E-FITC (all BioLegend), and live CD45⁺MHCII^–EpCAM^– lymphocytes were sorted using an Aria Fusion (BD). Samples were prepared using the Chromium Single Cell 3′ GEM, Library and Gel Bead kit v3 (10x Genomics). Cell viability was assessed using an EVE cell counter and trypan blue viability stain. Approximately 10,000 cells were loaded into the 10x Chromium, which was operated according to manufacturer’s instructions. Sequencing was performed on an Illumina HiSeq 4000 with a read configuration recommended by 10x Genomics for the sequencing of these libraries (28-base pair (bp) read 1, 98-bp read 2, 8-bp index 1).

McKenzie D.R., Hart R., Bah N., Ushakov D.S., Muñoz-Ruiz M., Feederle R, & Hayday A.C. (2022). Normality sensing licenses local T cells for innate-like tissue surveillance. Nature Immunology, 23(3), 411-422.

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Multi-Marker Immunophenotyping of T Cells

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Anti-CD3-AlexaFluor®700 (UCHT1), anti-CD4-APC-eFluor®480 (RPAT4), anti-CD69-biotin (FN50), anti-CD127-PerCP-Cy5.5 (eBioRDRS), anti-CD27-APC-eFluor®780 (LG.7F9), anti-CD4-FITC (RPA-T4), anti-CD27-PE-Cy7 (LG.7F9), anti-CD45-APC (2D1), and anti-EpCAM-PE (IB7), anti-FoxP3-PE-Cy7 (PCH101), anti-IL-2-PE-Cy7 (MQ1-17H12), anti-IFNγ-APC-eFluor®780 (4S.B3), anti-TNFα-PerCP-Cy5.5 (Mab11), anti-PD-1-APC (MIH4), anti-PD-L1-PE-Cy7 (MIH1), mIgG1κ-PE-Cy7 (P3.6.2.8.1), anti-CD45RAFITC (HI100), anti-CD45RO-biotin (UCHL1), CD62L-PE-Cy7 (DREG-56), CCR7-APC-efl780 (3D12), anti-CD3 (OKT3), and anti-CD28 (CD28.2) were purchased from eBioscience (San Diego, CA). Anti-CD8-BV510 (RPA-T8) was purchased from BioLegend (San Diego, CA). Anti-CTLA-4-Biotin and anti-Ki67-PerCP-Cy5.5 (B56) were purchased from BD Biosciences (San Diego, CA). Anti-TIM-3-PE (344823) was purchased from R&D systems. Anti-LAG-3-FITC (17B4) was purchased from Enzo Life Sciences International, Inc. (Plymouth Meeting, PA). Anti-CD107a-PE-Cy7 (H4A3) and anti-perforin-PerCP-Cy5.5 (δG9) were purchased from BD Biosciences. Anti-galectin-9 (ab69630) was purchased from Abcam. Control rabbit IgG (BA-1000) was purchased from Vector Labs (Burlingame, CA). Goat-anti-Rabbit IgG-HRP was purchased from Dako (Carpinteria, CA). All antibodies were used per the manufacturer's recommendations.

Severson J.J., Serracino H.S., Mateescu V., Raeburn C.D., C.McIntyre R J.r., Sams S.B., Haugen B.R, & French J.D. (2015). PD-1+Tim-3+ CD8+ T Lymphocytes Display Varied Degrees of Functional Exhaustion in Patients with Regionally Metastatic Differentiated Thyroid Cancer. Cancer immunology research, 3(6), 620-630.

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Flow Cytometric Analysis of Cell Markers

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The following antibodies were used for flow cytometric analysis of cells: anti-CD24-FITC, anti-CD44-FITC, anti-CD90-FITC, anti-CD133-PE, anti-MDR1-PE, anti-EpCAM-PE, anti-CD13-PE (all from eBioscience, San Diego, CA) and anti-CDCP1-FITC and anti-ABCG2-PE (both from BioLegend, San Diego, CA). Cells were incubated with the indicated antibodies for 60 min and were then washed twice with PBS containing 2% FCS. Flow cytometric analysis was performed using a FACS-Calibur (BD Immunocytometry Systems, Franklin Lakes, NJ).

Nohara S., Kato K., Fujiwara D., Sakuragi N., Yanagihara K., Iwanuma Y, & Kajiyama Y. (2016). Aminopeptidase N (APN/CD13) as a target molecule for scirrhous gastric cancer. Clinics and Research in Hepatology and Gastroenterology, 40(4), 494-503.

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