Milliplex map human adipokine magnetic bead panel 1 hadk1mag 61k

All the participants underwent physical, anthropometric and biochemical assessments. Blood extractions were performed by specialized nurses through a BD Vacutainer^® system, after overnight fasting before the surgery. Venous blood samples were obtained using empty and ethylenediaminetetraacetic acid coated tubes, which were, respectively, separated into serum and plasma aliquots by centrifugation (3500 rpm, 4 °C, 15 min) (Fisher Scientific SL, Madrid, Spain). Biochemical parameters were analyzed using a conventional automated analyzer. Insulin resistance was estimated using HOMA1-IR.
IL-1β, IL-6, IL-7, IL-8, IL-10, IL-13, IL-17, IL-22, tumor necrosis factor α (TNF-α), PAI-1, MCP-1, adiponectin and resistin were determined using multiplex sandwich immunoassays and the MILLIPLEX MAP Human Adipokine Magnetic Bead Panel 1 (HADK1MAG-61K, Millipore, Billerica, MA, USA) and MILLIPLEX MAP Human High- Sensitivity T Cell Panel (HSTCMAG28SK, Millipore, Billerica, MA, USA), and the Bio- Plex 200 instrument (Bio-Rad Laboratories SA, Madrid, Spain). at the Center for Omic Sciences (Universitat Rovira i Virgili), according to the manufacturer’s instructions.

Physical, anthropometric, and biochemical evaluation were performed on all the studied cohort. Blood samples were extracted through a BD Vacutainer^® system by specialized nurses, after overnight fasting and before bariatric surgery. Venous blood samples were obtained in tubes with or without ethylenediaminetetraacetic acid, which were separated in plasma and serum aliquots by centrifugation (3500 rpm, 4 °C, 15 min). Conventional automated analyser was used to analyse biochemical parameters. IR was estimated using homeostatic model assessment for IR (HOMA1-IR). Cytokines, such as interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-17, TNF-α and adiponectin, were determined using multiplex sandwich immunoassays and the MILLIPLEX MAP Human Adipokine Magnetic Bead Panel 1 (HADK1MAG-61K, Millipore, Billerica, MA, USA), the MILLIPLEX MAP Human High-Sensitivity T Cell Panel (HSTCMAG28SK, Millipore, Billerica, MA, USA), and the Bio-Plex 200 instrument, according to the manufacturer’s instructions. Absolute quantification of circulating bile acids, choline, trimethylamine (TMA), trimethylamine N-oxide (TMAO), betaine and short-chain fatty acids were analysed by liquid chromatography coupled to triple-quadrupole-mass spectrometry (LC-QqQ). All these analyses were assessed at the Center for Omic Sciences (Rovira i Virgili University-Eurecat).

Prior to bariatric surgery and having fasted the night before, specialized nurses collected blood samples into tubes with or without ethylenediaminetetraacetic acid through a BD Vacutainer^® system. These samples were then separated into aliquots of serum and plasma centrifugation (3500 rpm, 4 °C, and 15 min). A conventional automatic analyzer was used to analyze the biochemical parameters, and the IR was estimated through the homeostatic model for IR (HOMA1-IR). Cytokines, such as interleukin IL-6, IL-8, IL-10, TNF-α, and adiponectin, were determined in all samples of the cohort by multiplex sandwich immunoassays and MILLIPLEX MAP Human Adipokine Magnetic Bead Panel 1 (HADK1MAG-61K, Millipore, Billerica, MA, USA), the MILLIPLEX MAP High Sensitivity Human T-Cell Panel (HSTCMAG28SK, Millipore, Billerica, MA, USA), and the Bio-Plex 200 instrument, according to manufacturer’s instructions. All of these analyses were evaluated at the Omic Sciences Center (Eurecat, Reus, Spain), and the physical, anthropometric, and biochemical evaluations were performed on the entire cohort.