It is a triterpenoid quinone methide, and one of the bioactive components of the extracts prepared from the Celastraceae family of plants [25 (link)–28 (link)]. Purified Pristimerin (C30H40O4) was purchased from Pi and Pi Technology, Guangzhou, China. A stock solution of Pristimerin, 10 mg in 0.6 ml of dimethylsulfoxide (DMSO) (Sigma-Aldrich) was prepared and frozen at −20 °C in small aliquots until needed. PBS containing 1.2% DMSO was used both for dilution of the stock Pristimerin and as a ‘vehicle’ control for Pristimerin in different experiments. (For simplicity, PBS-DMSO has been denoted as PBS.) The dose of Pristimerin (1 mg/kg) used in vivo in this study is based on our titrations in a pilot study. The dose of Pristimerin used for in vitro testing was much below the dose that was cytotoxic for M3CT3 and lymph node cells (LNC) (data not shown).
Pristimerin
Manufactured by Merck Group
Sourced in United States
Pristimerin is a chemical compound used in laboratory settings. It functions as a triterpenoid compound with reported biological activities. No further details on its intended use or applications can be provided in an unbiased and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Anti parp 1, by Santa Cruz Biotechnology (1 mentions)
Anti survivin, by Santa Cruz Biotechnology (1 mentions)
Anti bcl xl, by Santa Cruz Biotechnology (1 mentions)
Anti bcl 2, by Santa Cruz Biotechnology (1 mentions)
Anti caspase 3, by BD (1 mentions)
Caspase 9 antibodies, by BD (1 mentions)
96 aqueous one solution proliferation assay system, by Promega (1 mentions)
Fitc annexin 5 apoptosis detection kit, by BD (1 mentions)
Annexin 5 fitc pi, by Merck Group (1 mentions)
Akt sirna, by RiboBio (1 mentions)
Cathepsin k, by Thermo Fisher Scientific (1 mentions)
Tnf α, by Thermo Fisher Scientific (1 mentions)
Osteocalcin, by Thermo Fisher Scientific (1 mentions)
Il 1β, by Thermo Fisher Scientific (1 mentions)
5 protocols using pristimerin
1
Extraction and Purification of Pristimerin
2
Pristimerin-Induced Apoptosis in Cancer Cells
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Pristimerin was purchased from Sigma Chemicals (St. Louis, MO). Anti-caspase-3 and caspase-9 antibodies were purchased from BD Pharmingen (San Diego, CA). Anti-PARP-1, anti-Bcl-2, anti-Bcl-xL and anti-survivin antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). 96 AQueous One Solution Proliferation Assay System was from Promega (Madison, WI). Annexin V-FITC apoptosis detection kit was purchased from BD Pharmingen and mitochondrial potential sensor JC-1 was obtained from Molecular Probes, Invitrogen (San Diego, CA). Caspase-3 activity kit was from Clontech Laboratories (Mountain View, CA). Stock solution of PM (100 mM) was prepared in DMSO and all test concentrations were prepared by diluting stock solution in tissue culture medium.
3
Pristimerin-Induced Apoptosis Pathway
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Pristimerin, Annexin V‐FITC/PI, JC‐1 were purchased from Sigma‐Aldrich. Akt‐siRNA was obtained from Ribobio. Plasmid for FoxO3a, FoxO3a siRNA and Empty plasmid vectors N1 were kindly provided by Marten P. Smidt (Rudolf Magnus Institute of Neuroscience).
4
Pristimerin and Alendronate Antiosteoporosis Study
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Pristimerin was provided by Sigma Aldrich Ltd., USA, and alendronate was provided by Hangzhou Moshadong Pharmaceutical Co. Ltd., China. ELISA kits with collagen type I fragments, osteocalcin, cathepsin K, BGP, TNF-α, and IL-1β were purchased from Thermo Fisher Scientific Ltd., USA. Antibodies were procured from Bio-Techne China Ltd., China.
5
High-Purity Pristimerin Procurement
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Pristimerin (purity: ≥98 % as determined by HPLC) was purchased from Sigma Aldrich (St Louis, MO, USA).
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