The TOV21G cell line was purchased from ATCC (Manassas, VA, USA) and cultured in MCDB105 medium (Zhong Qiao Xin Zhou Biotechnology, Shanghai, China). The OVCAR3 cell line was purchased from the National Collection of Authenticated Cell Cultures (Shanghai, China) and cultured in RPMI-1640 medium (Zhong Qiao Xin Zhou Biotechnology, Shanghai, China) supplemented with 10% FBS (Gibco, USA) and 1% penicillin/streptomycin (Invitrogen, USA). Both cell lines were maintained in an incubator at 37 °C with 5% CO2.
Rpmi 1640 medium
Manufactured by Shanghai Zhong Qiao Xin Zhou Biotechnology
Sourced in China
RPMI-1640 medium is a widely used cell culture medium formulation designed to support the growth and maintenance of a variety of cell types, including human and animal cell lines. It provides the necessary nutrients, vitamins, and other components required for optimal cell proliferation and survival in an in vitro environment.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Shanghai Zhong Qiao Xin Zhou Biotechnology (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Tov 21g cell line, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Sartorius (1 mentions)
Streptomycin, by Sartorius (1 mentions)
Eca109, by American Type Culture Collection (1 mentions)
Kyse450, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by Shanghai Zhong Qiao Xin Zhou Biotechnology (1 mentions)
Acrylamide, by J&K Scientific (1 mentions)
Tryptophan, by J&K Scientific (1 mentions)
N hexane, by J&K Scientific (1 mentions)
Glycine, by J&K Scientific (1 mentions)
Sterile culture flasks and plates, by Thermo Fisher Scientific (1 mentions)
F12k medium, by Shanghai Zhong Qiao Xin Zhou Biotechnology (1 mentions)
H1299, by Shanghai Zhong Qiao Xin Zhou Biotechnology (1 mentions)
Phorbol myristate acetate (pma), by MedChemExpress (1 mentions)
Thp 1 cells, by Shanghai Zhong Qiao Xin Zhou Biotechnology (1 mentions)
Fetal bovine serum (fbs), by GE Healthcare (1 mentions)
5 protocols using rpmi 1640 medium
1
Culturing TOV21G and OVCAR3 Cell Lines
2
Cell Culture of Esophageal Cancer Lines
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ECA109 and KYSE450 cells were obtained from the American Type Culture Collection, while KYSE150 cells were purchased from Shanghai Zhongqiao Xinzhou Biotechnology Co., Ltd. The culture medium used contained 89% RPMI 1640 medium (cat. no. ZQ0449; Zhongqiao Xinzhou Biotechnology Co., Ltd.), 10% fetal bovine serum (cat. no. 04-001-1ACS; Biological Industries), 1% 100 IU/ml penicillin and 100 g/ml streptomycin (cat. no. CSP006; Zhongqiao Xinzhou Biotechnology Co., Ltd.). Cells were cultured at 37°C and 5% CO2 saturated humidity.
3
Determination of Acrylamide in Food Samples
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Acrylamide (AA, ≥99%), lysine (Lys, ≥98%), tryptophan (Trp, ≥99%), GABA (≥98%), glycine (Gly, ≥99.5%) and n-hexane were purchased from J&K Scientific Co., Ltd (Beijing, China). Methanol (HPLC), D2O and DMSO were purchased from Energy Chemical Reagent Co., Ltd (Shanghai, China). A-HG type octadecyl bonded 1 silica gel was purchased from YMC Co., Ltd (Tokyo, Japan). Dextran gel Sephadex LH-20 was purchased from Extrasynthese Co., Ltd (Lyon, France). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), RPMI-1640 medium, fetal bovine serum (FBS) and penicillin-streptomycin were purchased from Shanghai Zhongqiao Xinzhou Biotechnology Co., Ltd, (China). Ten food products (Potato chips A, Potato chips B, Potato chips C, brown rice cake, egg biscuit, cereal bars, dough twist, walnut cake and rice crust) were purchased from Utilize modern supermarkets (Guangzhou, China).
4
Cell Culture of Lung Cancer Lines
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H1299 and A549 lung cancer cell lines were purchased from Zhong Qiao Xin Zhou Biotechnology (Shanghai, China). A549 cells were cultured in F12K medium (Zhong Qiao Xin Zhou Biotechnology) and H1299 cells were cultured in RPMI 1640 medium (Zhong Qiao Xin Zhou Biotechnology). Both media were supplemented with 10% foetal bovine serum (FB15015; Clark Biosciences, Richmond, VA, USA). Sterile culture flasks and plates (Thermo Fisher Scientific, Waltham, MA, USA) were used to culture the cells. The cell lines were cultured as described previously 26 (link).
5
Culturing and Differentiating THP-1 and 293T Cells
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Human monocytic THP-1 cells were purchased from Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. and suspended in RPMI-1640 medium (Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd.) containing 10% FBS (GEHealthcare Life Sciences) maintained at 37°C in a humidified atmosphere containing 5% CO2. THP-1 cells were induced to differentiate into macrophages using the phorbol 12-myristate 13-acetate (PMA; MedChemExpress LLC; 100 nM) (22 (link)), for 48 h at 37°C prior to subsequent experimentation.
293T cells (Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd.) were cultured in DMEM (Gibco; Thermo Fisher Scientific, Inc.) with 10% FBS and maintained at 37°C in a humidified atmosphere containing 5% CO2.
293T cells (Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd.) were cultured in DMEM (Gibco; Thermo Fisher Scientific, Inc.) with 10% FBS and maintained at 37°C in a humidified atmosphere containing 5% CO2.
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