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8 protocols using cf3cooh

1

Copper Carboxylate Synthesis and Characterization

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CuCO3·Cu(OH)2 (>95%), anhydrous acetonitrile (99.8%), CF3COOH (99%), and RCOOH (R = CnF2n+1; n = 2–4; 97–98%) were purchased from Sigma Aldrich (Saint Louis, MO, USA) and used as received. Pentafluoropropylamidine C2F5C(=NH)NH2 (AMDH) (98.7%) was purchased from Apollo Scientific (Stockport, UK) and used as received. Copper(II) carboxylates [Cu2(µ-O2CRF)4], where RF = CnF2n+1, n = 1–4, were prepared as earlier reported [46 (link)].
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2

Synthesis of Ln-Doped Polystyrene Beads

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CF3COOH, Ln2O3 (Ln = Sc, Y, Gd, Yb, Er, Ho and Tm), Ln(CH3COO)3·4H2O, CF3COOK·H2O, oleic acid (OA), oleylamine (OM), and 1-octadecence (ODE) were purchased from Sigma-Aldrich (China). Ln(CF3COO)3·4H2O was prepared as reported in the literature.41 Polystyrene beads (9.0–9.9 μm) were purchased from Aladdin Reagent Co., Ltd. 1-Butanol, dichloromethane, cyclohexane, NaOH, NH4F, and ethanol were purchased from Sinopharm Chemical Reagent Co., China. Unless otherwise noted, all of the chemical reagents were used as received without further purification.
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3

Synthetic Peptide Production Protocol

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Protected amino acids were purchased from Chem-Impex International (Chicago, IL). H-Cys (Trt)-2-Cl-Trt resin was obtained from Novabiochem (San Diego, CA). 2-(1H-7-Azabenzotriazole-1-yl)-1,1,3,3-tetramethyl uronium hexafluorophosphate methanaminium (HATU) was obtained from Genscript Inc. (Piscataway, NJ). Sulfuric acid, methanol, DMSO, DMF, TFA, isopropyl alcohol, NH2-PEG12-COOH-tBu, diisopropylethylamine (DIPEA), piperidine, CF3COOH, CH2Cl2, K2CO3, tyramine and all other chemical reagents were purchased from Sigma Aldrich. Pure coat Amine 24-well microtiter plates were purchased from BD Biosciences (San Jose, CA). All other cell culture reagents, syringes and disposable items were purchased from VWR (Chicago, IL).
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4

Theranostic Cubosomes Preparation

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The following chemicals for
the preparation of theranostic cubosomes were purchased from Sigma-Aldrich:
daunorubicin hydrochloride (DNR), Pluronic F108 (PF108), chitosan
(CHIT, low molecular weight), DNA sodium salt from herring testes
(DNA), Y2O3 (99.99%), Yb2O3 (99.99%), Er2O3 (99.99%), CF3COOH
(reagent grade, 99%), oleic acid (technical grade, >93%), oleylamine
(technical grade, 70%), folic acid (reagent grade, ≥97%). Monoolein
(MO, 1-monooleoylglycerol, RYLO MG 19PHARMA, glycerol monooleate;
98.1 wt %) was a kind gift from Danisco A/S, DK-7200, Grinsted, Denmark.
Other reagents and solvents were of commercial grade and were used
as received. The water used for all experiments was doubly distilled
and purified by means of a Milli-Q purification system (Millipore,
Bedford, MA).
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5

Chemical Reagents and Materials

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2-(1H-7-Azabenzotriazole-1-yl)-1,1,3,3-tetramethyl uronium hexafluorophosphate methanaminium (HATU) was obtained from Genscript Inc. (Piscataway, NJ). Sulfuric acid, methanol, DMSO, DMF, TFA, isopropyl alcohol, NH2-PEG12-COOH-tBu, diisopropylethylamine (DIPEA), piperidine, CF3COOH, CH2Cl2, K2CO3, tyramine and all other chemical reagents were purchased from Sigma Aldrich. Purecoat Amine 24-well microtiter plates were purchased from BD Biosciences (San Jose, CA). All other cell culture reagents, syringes and disposable items were purchased from VWR (Chicago, IL).
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6

Molecular Mechanisms of Neuroinflammation

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DMEM was purchased from Hyclone (Beijing, China). Fetal bovine serum (FBS) was purchased from BI (Israel). Griess reagent was purchased from Beyotime (Shanghai, China). LPS, RIPA buffer, and CF3COOH (TFA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Rabbit antibodies against mouse iNOS, COX-2, Tau, p-Tau, IBA-1 TREM2, DAP12, TLR4, MyD88, Caspase-1, and NLRP3 were obtained from Abclonal (Wuhan, China). HPLC-grade methanol and acetonitrile were supplied from Merck KGaA (Darmstadt, Germany). PBS, CM5 sensing chips were obtained from from GE Healthcare (Sweden). HP-20 macroporous resin was purchased from Mitsubishi Chemical Company (Japan).
An ultrasonic cleaner, high-speed freezing centrifuge, and electric heater were obtained from Branson (EYELA, Tokyo, Japan), and Biacore T200 (GE Healthcare, Sweden). The Xevo G2-XS Qtof was acquired from Waters Inc (Milford, USA.). Deionized water was collected from a Milli-Qwater purification system (Merck KGaA, Darmstadt, Germany). The rotavapor tandem cold trap was from EYELA (EYELA, Tokyo, Japan). The Epoch 2 microplate reader was got by BioTek (USA). The Electrophoresis Device and scanning imager were acquired from BIO-RAD.
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7

Synthesis of Lead-based Perovskites

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RbF, CsF (Alfa Aesar, 99.5%), Pb(OAc)2·3H2O, PbF2, PbCO3 (Sigma-Aldrich, 99.9%), MeOH
(Sigma-Aldrich, 99%), and CF3COOH (Sigma-Aldrich, 99%)
were used as starting materials.
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8

Synthesis of Lanthanide-Doped Nanoparticles

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IR-780 iodide (99%), 4-mercaptobenzoic acid (99%), Y2O3 (99.9%), Yb2O3 (99.9%), Er2O3 (99.9%), CF3COONa (99.9%), CF3COOH, 1-octadecene (90%), oleic acid (90%), oleylamine (90%), N,N-dimethylformamide (DMF, anhydrous, 99.8%), and dichloromethane (DCM, AR grade) were all purchased from Sigma-Aldrich and used without further purification. The lanthanide (Ln) trifluoroacetates, Ln(CF3COO)3, were prepared as described in the literature.10 (link)
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