Spectromonitor 3200

Manufactured by Shimadzu

Sourced in Japan

The SpectroMonitor 3200 is a spectrophotometer designed for laboratory use. It measures the absorption or transmission of light by a sample at specific wavelengths. The device is capable of analyzing a wide range of materials and solutions.

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Lab products found in correlation

Shim pack gis 0.5 mm ods c18 column, by Shimadzu (1 mentions)

2 protocols using spectromonitor 3200

HPLC Analysis of Compounds

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For HPLC analysis, a Shimadzu SpectroMonitor 3200 digital UV-Vis detector equipped with a Shim-pack GIS 0.5 mm ODS C18 column (250 × 4.6 mm I.D.) was used (Shimadzu, Kyoto, Japan). The mobile phase consisted of water with 0.1% TFA (Solvent A) and acetonitrile (Solvent B). The gradient method was used with a flow rate of 1 mL/min and the analysis was done by increasing solvent B from 10% to 100% for 30 min.

Park T., Park J.S., Sim J.H, & Kim S.Y. (2020). 7-Acetoxycoumarin Inhibits LPS-Induced Inflammatory Cytokine Synthesis by IκBα Degradation and MAPK Activation in Macrophage Cells. Molecules, 25(14), 3124.

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HPLC Analysis of Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

HPLC analysis was performed using a Shimadzu SpectroMonitor 3200 equipped with a Shim-pack GIS 0.5 mm ODS C18 column (250 × 4.6 mm id), with the mobile phase consisting of solvent A (0.1% v/v trifluoroacetic acid in water) and B (acetonitrile). A gradient method was used in which the flow rate of solvent B (1 mL/min) was increased from 10/90 to 50/50 over 30 min. The column temperature was maintained at 40 °C. The wavelength was measured at 254 nm.

Kim J.H., Park T.J., Park J.S., Kim M.S., Chi W.J, & Kim S.Y. (2021). Luteolin-3′-O-Phosphate Inhibits Lipopolysaccharide-Induced Inflammatory Responses by Regulating NF-κB/MAPK Cascade Signaling in RAW 264.7 Cells. Molecules, 26(23), 7393.

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