Log In Sign up
The largest database of trusted experimental protocols

Anti mir nc

Manufactured by GeneCopoeia
Visit Supplier
Sourced in China

Anti-miR-NC is a negative control for miRNA inhibition experiments. It is a non-targeting small RNA molecule designed to have no known gene targets in mammalian cells.

Automatically generated - may contain errors

Lab products found in correlation

Mir nc, by GeneCopoeia (6 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (3 mentions) Si nc, by Keygen Biotech (3 mentions) Sh nc, by GeneCopoeia (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) A2058, by American Type Culture Collection (1 mentions) Pcdna3.1 vector, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Sh nc, by RiboBio (1 mentions) Ab16667, by Abcam (1 mentions) Balb c nude mice, by Charles River Laboratories (1 mentions) In situ cell death detection kit, by Roche (1 mentions) Lipofectamine 3000, by Solarbio (1 mentions) Pcdna3.1 vector, by Promega (1 mentions) Traf6, by GeneCopoeia (1 mentions) Si nc, by GeneCopoeia (1 mentions)

9 protocols using anti mir nc

1

Overexpression and Silencing of Circular FOXM1 in Melanoma

Check if the same lab product or an alternative is used in the 5 most similar protocols
Normal human epidermal melanocytes (HEMn) and melanoma cells (A2058 and A375) were all bought from the American Type Culture Collection (ATCC, Manassas, VA, USA). These cells were grown in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS; Gibco) and 1% penicillin-streptomycin (Gibco) at 37 °C in a humidified incubator with 5% CO2.
The overexpression vector of circ-FOXM1 (circ-FOXM1), the overexpression vector of FLOT2 (FLOT2) and their control (pcDNA), small interfering RNA (siRNA) against circ-FOXM1 (si-circ-FOXM1) and negative control (si-NC), mimics of miR-143-3p (miR-143-3p) and control mimic (miR-NC), inhibitors of miR-143-3p (anti-miR-143-3p) and its control (anti-miR-NC), and short hairpin RNA against circ-FOXM1 (sh-circ-FOXM1) and its control (sh-NC) were bought from GeneCopoeia (Guangzhou, China). The synthetic vectors or oligonucleotides were transfected into cells using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA).
Tian S., Han G., Lu L, & Meng X. (2020). Circ-FOXM1 contributes to cell proliferation, invasion, and glycolysis and represses apoptosis in melanoma by regulating miR-143-3p/FLOT2 axis. World Journal of Surgical Oncology, 18, 56.
+ Open protocol
+ Expand
2

Modulating hsa_circ_0010235 expression in NSCLC

Check if the same lab product or an alternative is used in the 5 most similar protocols
To knock down hsa_circ_0010235 expression in H1299 and A549 cells, small interference RNAs against hsa_circ_0010235 (si-hsa_circ_0010235#1 and si-hsa_circ_0010235#2; KeyGEN Biotech, Nanjing, China) were introduced, with si-NC (KeyGEN Biotech) as negative control. To overexpress hsa_circ_0010235, its overexpression vector (hsa_circ_0010235; GenePharma Co. Ltd., Shanghai, China) was applied, with pCD-ciR (GenePharma Co. Ltd.) empty vector as negative control. MiR-433-3p mimic (miR-433-3p), miR-433-3p inhibitor (anti-miR-433-3p) and their respective negative control (miR-NC and anti-miR-NC) were obtained from GeneCopoeia (Guangzhou, China). Overexpression vector of TIPRL (TIPRL) was constructed by inserting its full-length sequence into pcDNA 3.1 vector (Invitrogen, Carlsbad, CA, USA). Above-mentioned plasmids or oligonucleotides were introduced into NSCLC cells utilizing Lipofectamine 3000 (Invitrogen) following the user’s manual.
Zhang F., Cheng R., Li P., Lu C, & Zhang G. (2021). Hsa_circ_0010235 functions as an oncogenic drive in non-small cell lung cancer by modulating miR-433-3p/TIPRL axis. Cancer Cell International, 21, 73.
+ Open protocol
+ Expand
3

Establishing and Manipulating Docetaxel-Resistant Prostate Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Four PCa cell lines (22Rv1, VCaP, DU145, and PC3) and human normal prostate epithelial cells (RWPE-1) were obtained from COBIOER (Nanjing, China). All cells were allowed to grow in RPMI 1640 medium (Invitrogen, Carlsbad, CA, USA) that contained 10% FBS (Sigma-Aldrich, St. Louis, MO, USA) in a humidified air with 5% CO2 at 37°C. To establish DTX-resistant PCa cells (DU145/DTX and PC3/DTX), DU145 and PC3 cells were exposed to increasing doses of DTX (Sigma-Aldrich). Then, DTX-resistant cells were treated with DTX (5 nM) to maintain a resistant phenotype.
Small interference RNA against circ-XIAP (si-circ-XIAP) and matched negative control (si-NC), circ-XIAP or TPD52 overexpression vector (circ-XIAP or TPD52) and matched negative control (vector), miR-1182 mimics (miR-1182) and match negative control (miR-NC), miR-1182 inhibitor (anti-miR-1182) and match negative control (anti-miR-NC) were synthesized by GeneCopoeia (Guangzhou, China). Lentivirus packaged plasmid sh-circ-XIAP and its negative control (sh-NC) were provided by RiboBio (Guangzhou, China). Cells were transfected by using Lipofectamine 3000 (Invitrogen).
Zhang H., Li M., Zhang J., Shen Y, & Gui Q. (2021). Exosomal Circ-XIAP Promotes Docetaxel Resistance in Prostate Cancer by Regulating miR-1182/TPD52 Axis. Drug Design, Development and Therapy, 15, 1835-1849.
+ Open protocol
+ Expand
4

In Vivo Xenograft Model of Liver Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
BALB/c nude mice (male, four-week-old) were obtained from Vital River Laboratory Animal Technology (Beijing, China) and housed in specific pathogen-free conditions with a 12 h light/dark cycle and free access to water and food. Every experiment was made to minimize animals (n = 7 per group) under the approval of the Animal Research Committee of Luoyang Central Hospital Affiliated to Zhengzhou University. HepG2 cells were transfected with the lentiviral vectors with anti-miR-196a (Lenti-anti-miR-196a), anti-miR-196b (Lenti-anti-miR-196b), anti-miR-NC (Lenti-anti-miR-NC), or negative control (Lenti-NC) constructed by GeneCopoeia (Rockville, MD, USA). Stably transfected cells (5 × 106) were subcutaneously injected into the nude mice. Tumor volumes were measured every week for 5 weeks with the formula: volume (mm3) = width2 × length/2. After 5 weeks following the inoculation, the mice were killed and tumor samples were collected and weighted. The collected tumor tissues were used for further molecular studies. Cell apoptosis and proliferation in tumor tissues were measured by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining with in situ cell death detection kit (Roche, Mannheim, Germany) or Ki67 immunohistochemistry with anti-Ki67 antibody (ab16667, 1:1000 dilution, Abcam) following the manufacturer’s instructions as previous study20 (link).
Ren W., Wu S., Wu Y., Liu T., Zhao X, & Li Y. (2019). MicroRNA-196a/-196b regulate the progression of hepatocellular carcinoma through modulating the JAK/STAT pathway via targeting SOCS2. Cell Death & Disease, 10(5), 333.
+ Open protocol
+ Expand
5

Modulation of Circular RNA circ_CDR1as and Raf1 in Liver Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
To silence circ_CDR1as or Raf1, small interference RNA (siRNA) targeting circ_CDR1as (si-circ_CDR1as) or Raf1 (si-Raf1) and the negative control (si-NC) are synthesized by KeyGEN Biotech (Nanjing, China). For overexpression, overexpression vector of circ_CDR1as (circ_CDR1as) and Raf1 (Raf1) and their corresponding negative control Vector and pcDNA are supplied by Hanbio Biotechnology Co., ltd (Shanghai, China). Furthermore, miR-1287 mimic (miR-1287) and the negative control (miR-NC), miR-1287 inhibitor (anti-miR-1287) and the negative control (anti-miR-NC) are constructed by GeneCopoeia (Guangzhou, China). These oligonucleotides or plasmids are introduced into Hep3B and Huh7 cells using Lipofectamine 3000 (Solarbio) according to the recommended protocols.
Zhang B., Li F., Zhu Z., Ding A, & Luo J. (2020). CircRNA CDR1as/miR-1287/Raf1 Axis Modulates Hepatocellular Carcinoma Progression Through MEK/ERK Pathway. Cancer Management and Research, 12, 8951-8964.
+ Open protocol
+ Expand
6

Modulation of circPITX1 and miR-329-3p in Glioma

Check if the same lab product or an alternative is used in the 5 most similar protocols
Until 60% of coverage, glioma U251 and LN229 cells were transfected with Lipofectamine 3000 (Invitrogen). For silencing circPITX1, small interfering RNA (siRNA) of circPITX1 (si-circPITX1) and the control (si-NC) were synthesized by KeyGEN Biotech (Jiangsu, China). For up-regulating circPITX1 or NEK2, the sequence was cloned into pcDNA3.1 vector (Promega, Southampton, UK), namely pcDNA-circPITX1 or pcDNA-NEK2, generating in Hanbio Biotechnology Co., ltd (Shanghai, China), with pcDNA-NC as control. MiR-329-3p inhibitors (anti-miR-329-3p), miR-329-3p mimics (miR-329-3p) and their paired control (anti-miR-NC and miR-NC) were obtained from GeneCopoeia.
Guan Y., Cao Z., Du J., Liu T, & Wang T. (2020). Circular RNA circPITX1 knockdown inhibits glycolysis to enhance radiosensitivity of glioma cells by miR-329-3p/NEK2 axis. Cancer Cell International, 20, 80.
+ Open protocol
+ Expand
7

Modulation of Chondrocyte Homeostasis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Circ-BRWD1 short interfering RNA (si-circ-BRWD1) and scramble control (si-NC), the overexpression vector of circ-BRWD1 (circ-BRWD1) and its control (pCD5-ciR), miR-1277 mimics (miR-1277) and control mimics (miR-NC), miR-1277 inhibitors (anti-miR-1277) and anti-miR-NC, the overexpression vector of TRAF6 (TRAF6) and pcDNA were purchased from GeneCopoeia. CHON-001 cells (1 × 104 cells/well) were seeded into 24-well plates and the oligonucleotides (50 nM) or vectors (2 μg) were transfected into CHON-001 cells utilizing Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturers’ instructions.
Guo Z., Wang H., Zhao F., Liu M., Wang F., Kang M., He W, & Lv Z. (2021). Exosomal circ-BRWD1 contributes to osteoarthritis development through the modulation of miR-1277/TRAF6 axis. Arthritis Research & Therapy, 23, 159.
+ Open protocol
+ Expand
8

Modulation of TUG1 and miR-17-5p in Chondrocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Small interfering RNA (siRNA) targeting TUG1 (si-TUG1; 5'-CCAUCUCACAAGGCUUCAATT-3'), FUT1 (si-FUT1; 5'-UCGAUGUUUUCUUUACACCAC-3') and controls (si-NC; 5'-UUCUCCGAACGUGUCACGUTT-3'); the pcDNA3.1-TUG1 overexpression vector (pc-TUG1) and corresponding empty vector (vector); miR-17-5p mimics (miR-17-5p; 5'-CAAAGUGCUUACAGUGCAGGUAG-3') and controls (miR-NC; 5'-UUCUCCGAACGUGUCACGUTT-3'); and miR-17-5p inhibitors (anti-miR-17-5p; 5'-CAAAGUGCUUACAGUGCAGGUAG-3') and controls (anti-miR-NC; 5'-CAGUACUUUUGUGUAGUACAA-3') were purchased from GeneCopoeia, Inc. Chondrocytes were seeded into 24-well plates at a density of 1.0x104 cells/well and the oligonucleotides (50 nM) or vectors (2 µg) were transfected into chondrocytes using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.).
Chondrocytes were stimulated with 10 ng/ml IL-1β (Beyotime Institute of Biotechnology) for 24 h at 37˚C. Untreated normal primary chondrocytes were used as controls.
Li Z., Wang J, & Yang J. (2020). TUG1 knockdown promoted viability and inhibited apoptosis and cartilage ECM degradation in chondrocytes via the miR-17-5p/FUT1 pathway in osteoarthritis. Experimental and Therapeutic Medicine, 20(6), 154.
+ Open protocol
+ Expand
9

Modulating miR-519a Expression and PTEN Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
miRNA vectors, including miR-519a expression vector (HmiR0342-MR04), the control vector for miR-519a (CmiR0001-MR04, miR-control), miR-519a inhibitor (HmiR-AN0588-AM04, anti-miR-519a) and the negative control for the miR-519a inhibitor (CmiR-AN0001-AM04, anti-miR-NC), and PTEN expression plasmid (EX-I0450-M02-5) were purchased from Genecopoeia (Guangzhou, China). The PTEN siRNA duplex sequence, 5′-GUU AGC AGA AAC AAA AGG AGA UAU CAA-3′ (sense)/5′-UUG AUA UCU CCU UUU GUU UCU GCU AAC-3′ (antisense) and a nonspecific duplex oligonucleotide as a negative control were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China). Cells were transfected with oligonucleotides using Lipofectamine 2000 Reagent (Invitrogen Life Technologies) following the manufacturer's instructions.
TU K., LIU Z., YAO B., HAN S, & YANG W. (2015). MicroRNA-519a promotes tumor growth by targeting PTEN/PI3K/AKT signaling in hepatocellular carcinoma. International Journal of Oncology, 48(3), 965-974.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!