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Jnk sc 571

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The JNK (sc-571) is a laboratory product manufactured by Santa Cruz Biotechnology. It is used as a research tool to identify and study the c-Jun N-terminal kinase (JNK) protein, which is involved in various cellular processes. The product provides a specific and reliable way to detect and analyze the JNK protein in experimental settings.

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Lab products found in correlation

Phospho p53, by Cell Signaling Technology (3 mentions) Perk t202 y204, by Cell Signaling Technology (2 mentions) P mtors2448, by Cell Signaling Technology (2 mentions) Hsp70, by Cell Signaling Technology (2 mentions) α tubulin, by Merck Group (2 mentions) P p38 t180 y182, by Cell Signaling Technology (2 mentions) Pan akt, by Cell Signaling Technology (2 mentions) P jnk s63 73, by Santa Cruz Biotechnology (2 mentions) Ap2053a, by Abcepta (2 mentions) P her2 y1278, by Cell Signaling Technology (2 mentions) Pakt s473, by Cell Signaling Technology (2 mentions) β actin, by Merck Group (2 mentions) E cadherin, by BD (2 mentions) P hsf 1 s326, by Abcam (2 mentions) P38 sc 7149, by Santa Cruz Biotechnology (2 mentions) Phospho jnk, by Cell Signaling Technology (2 mentions) Phospho p38, by Cell Signaling Technology (2 mentions) Mtor substrates sampler kit cst 9862, by Cell Signaling Technology (2 mentions) E cadherin, by Cell Signaling Technology (2 mentions) Gapdh 60004 1 ig, by Proteintech (2 mentions)

6 protocols using jnk sc 571

1

Immunoblotting and Chromatin Immunoprecipitation Assays

Cited 1 time
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This was performed as described previously.35 (link), 36 (link) Antibodies used in WB included mouse monoclonal antibodies against β-actin (Sigma), α-tubulin (Sigma), E-cadherin (610404, BD), p-HSF-1/S326 (ab76076, Epitomics/Abcam), p-JNK/S63/73 (sc-6254; Santa Cruz), as well as, rabbit antibodies against Slug (AP2053a,ABGENT), HSF-1 (4356, Cell Signaling), Hsp70 (4876, Cell Signaling), p38 (9212, Cell Signaling), p-p38/T180/Y182 (4511, Cell Signaling), pERK/T202/Y204 (9109, Cell Signaling), Akt/pan (4691, Cell Signaling), p-Akt/S473 (4060, Cell Signaling), mTOR (2983, Cell Signaling), p-mTOR/S2448 (5536, Cell Signaling), JNK (sc-571, Santa Cruz), HER2 (2165, Cell Signaling), p-HER2/Y1278 (2247, Cell Signaling). Rabbit polyclonal HSF-1 antibody used in IP and ChIP assay was from Cell signaling (4356).
Carpenter R.L., Paw I., Dewhirst M.W, & Lo H.W. (2014). Akt phosphorylates and activates HSF-1 independent of heat shock, leading to Slug overexpression and epithelial-mesenchymal transition (EMT) of HER2-overexpressing breast cancer cells. Oncogene, 34(5), 546-557.
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2

Western Blot Analysis of Liver Proteins

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Whole-cell lysates of liver tissue or cells were obtained using RIPA lysis buffer containing protease and phosphatase inhibitors. Protein samples were separated by 5% or 10% SDS-PAGE. Then, the proteins were transferred onto a polyvinylidene fluoride membrane and incubated with primary antibodies at 4 °C overnight. Corresponding secondary antibodies were applied to the membrane and incubated for 1 h. Protein bands were visualized by chemiluminescence. The following primary antibodies were used: JNK (sc-571; Santa Cruz Biotechnology), P-JNK (sc-6254; Santa Cruz Biotechnology), TGF-β1 (ab106582, Abcam), αSMA (sc-53142; Santa Cruz Biotechnology), and β-actin (sc-47778, Santa Cruz Biotechnology).
Liang Q.S., Xie J.G., Yu C., Feng Z., Ma J., Zhang Y., Wang D., Lu J., Zhuang R, & Yin J. (2021). Splenectomy improves liver fibrosis via tumor necrosis factor superfamily 14 (LIGHT) through the JNK/TGF-β1 signaling pathway. Experimental & Molecular Medicine, 53(3), 393-406.
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3

Immunoblotting and Chromatin Immunoprecipitation Assays

Cited 1 time
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This was performed as described previously.35 (link), 36 (link) Antibodies used in WB included mouse monoclonal antibodies against β-actin (Sigma), α-tubulin (Sigma), E-cadherin (610404, BD), p-HSF-1/S326 (ab76076, Epitomics/Abcam), p-JNK/S63/73 (sc-6254; Santa Cruz), as well as, rabbit antibodies against Slug (AP2053a,ABGENT), HSF-1 (4356, Cell Signaling), Hsp70 (4876, Cell Signaling), p38 (9212, Cell Signaling), p-p38/T180/Y182 (4511, Cell Signaling), pERK/T202/Y204 (9109, Cell Signaling), Akt/pan (4691, Cell Signaling), p-Akt/S473 (4060, Cell Signaling), mTOR (2983, Cell Signaling), p-mTOR/S2448 (5536, Cell Signaling), JNK (sc-571, Santa Cruz), HER2 (2165, Cell Signaling), p-HER2/Y1278 (2247, Cell Signaling). Rabbit polyclonal HSF-1 antibody used in IP and ChIP assay was from Cell signaling (4356).
Carpenter R.L., Paw I., Dewhirst M.W, & Lo H.W. (2014). Akt phosphorylates and activates HSF-1 independent of heat shock, leading to Slug overexpression and epithelial-mesenchymal transition (EMT) of HER2-overexpressing breast cancer cells. Oncogene, 34(5), 546-557.
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4

Western Blot Analysis of Signaling Pathways

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Western blot analysis was performed according to the standard protocols. Briefly, aliquots of total protein (30 μg) were electrophoresed on sodium dodecyl sulfate polyacrylamide, appropriate Tris–HCl gels. The separated proteins were transferred to polyvinylidene difluoride membranes (Millipore Co, Billerica, MA, USA) and incubated with primary antibodies for 2 h. Chemiluminescent detection was performed, and images were captured by LAS-3000 system (Fujifilm, Tokyo, Japan). Antibodies against β-catenin (51067-2-AP1:800), LC3A/B (66139-1-1g, 1:1000), and GAPDH (60004-1-Ig,1:1000) were from Proteintech. Antibodies against MEK1/2 (9126, 1:1000), Phospho-MEK1/2 (2338, 1:1000), Phospho-ERK1/2 (4376, 1:1000), AKT (4691.1:1000), Phospho-AKT (4060P,1:1000), Phospho-P38 (4511.1:1000), Phospho-JNK (4668,1:1000), E-cadherin (3195,1:1000), Phospho-p53 (9284.1:1000), and mTOR Substrates Sampler kit (CST 9862) that conclude Phospho-mTOR, mTOR, Phospho-p70s6389 (Thr389), Phospho-P70389 (Thr371), Phospho-4EBP1 (Thr37/46), and anti-rabbit IgG were from Cell Signaling Technology. Antibodies against p38 (sc-7149,1:1000), P53 (sc-126,1:1000), and JNK (sc-571, 1:1000) were from Santa Cruz Biotechnology (Dallas TX, USA).
Yang X.L., Qi L.G., Lin F.J, & Ou Z.L. (2017). The role of the chemokine receptor XCR1 in breast cancer cells. Breast Cancer : Targets and Therapy, 9, 227-236.
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5

Delineating Apoptotic Signaling Pathways

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PR-619 was obtained from MedChemExpress (Junction, NJ, USA) and cisplatin from Merck Millipore (Billerica, MA, USA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Merck Millipore. For Western blot analysis, antibodies against cleaved caspase-3 (#9661), cleaved caspase-8 (#9496), B cell lymphoma (Bcl)-2 (#15071), phospho-Bcl-2 (#2824), phospho-p53 (#9284), caspase-4 (#4450), phospho-Janus kinase (JNK) (#9255), and c-Myc (#18683) were procured from Cell Signaling Technology (Danvers, MA, USA). The antibodies against β-actin (#109) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, #100118) were supplied by GeneTex (Irvine, CA, USA); those against α-tubulin (sc-5286), P21 (sc-6264), P27 (sc-1641), and JNK (sc-571) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). For IHC, the USP14 antibody (#MA5-32821) was purchased from Invitrogen, the USP21 antibody (#17856-1-AP) was purchased from Proteintech (Chicago, IL, USA), and the c-Myc (#ab32072) antibody was purchased from Abcam (Cambridge, UK).
Hsu F.S., Lin W.C., Kuo K.L., Chiu Y.L., Hsu C.H., Liao S.M., Dong J.R., Liu S.H., Chang S.C., Yang S.P., Chen Y.T., Chang R.J, & Huang K.H. (2021). PR-619, a General Inhibitor of Deubiquitylating Enzymes, Diminishes Cisplatin Resistance in Urothelial Carcinoma Cells through the Suppression of c-Myc: An In Vitro and In Vivo Study. International Journal of Molecular Sciences, 22(21), 11706.
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6

Gemcitabine-Induced Signaling Pathway Analysis

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Gemcitabine was purchased from Lilly France (St-Cloud, France) (Lot No A875303A). Antibodies against β-catenin (51067-2-AP, 1:800), LC3A/B (66139-1-1g, 1:1,000), Cyclin D1 (60186-1-1g, 1:1,000) and GAPDH (60004-1-Ig, 1:1,000) were from Proteintech Group, Inc., (Chicago, IL, USA). Antibodies against MEK1/2 (9126, 1:1,000), Phospho-MEK1/2 (2338, 1:1,000), Phospho-ERK1/2 (4376, 1:1,000), AKT (4691, 1:1,000), Phospho-AKT (4060P, 1:1,000), Phospho-P38 (4511, 1:1,000), Phospho-JNK (4668, 1:1,000), E-Cadherin (3195, 1:1,000), Phospho-p53 (9284, 1:1,000), and mTOR Substrates Sampler kit (CST 9862), which includes Phospho-mTOR, mTOR, Phospho-p70s6389 (Thr389), Phospho-P70389 (Thr371), Phospho-4EBP1 (Thr37/46), and anti-rabbit IgG, were purchased from Cell Signaling Technology, Inc., (Danvers, MA, USA). Antibodies against p38 (sc-7149, 1:1,000), P53 (sc-126, 1:1,000) and JNK (sc-571, 1:1,000) were from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). PD98059 (MEK inhibitor, 9900, 20 UM), rapamycin (mTOR inhibitor, 9904, 10 nM), and LY294002 (PI3K Inhibitor, 9901, 50 μM) were purchased from Cell Signaling Technology.
Yang X.L., Lin F.J., Guo Y.J., Shao Z.M, & Ou Z.L. (2014). Gemcitabine resistance in breast cancer cells regulated by PI3K/AKT-mediated cellular proliferation exerts negative feedback via the MEK/MAPK and mTOR pathways. OncoTargets and therapy, 7, 1033-1042.
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