Axiovert 200 M fluorescent light microscope for both brain and liver (Zeiss,
Thornwood, NY) equipped with an Axiocam b/w camera as previously described for
prefrontal cortex (37 (link)). Parameters used
for image acquisition were identical across treatments. To quantify K1 synthetic
dsRNA counts and fluorescence intensity in prefrontal cortex and liver, a single
in-focus plane was acquired and analyzed as previously described using Image J
(v1.49u, NIH) (37 (link), 38 (link)).