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4 protocols using ellagic acid

1

Quantification of FXII and PK Activation

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Materials used were as follows: normal human plasma (Precision BioLogic, Dartmouth, Canada); FXII-deficient plasma (George King Biomed); FXII, FXIIa, βFXIIa, PK, PKa, α-thrombin, corn trypsin inhibitor, and horseradish peroxidase–conjugated goat anti-human FXII and anti-human PK (Enzyme Research Laboratory, South Bend, IN); dextran sulfate (500 000 Da), polyethyleneimine (PEI; 750 000 Da), soybean trypsin inhibitor, and ε-aminocaproic acid (ε-ACA; Sigma-Aldrich, St. Louis, MO); ellagic acid (ACROS Organics, The Hague, Netherlands); S2302 (H-d-prolyl-l-phenylalanyl-l-arginine-p-nitroanilide) and S-2366 (l-pyro-Glu-l-Pro-l-Arg-p-nitroanilide; DiaPharma, Westchester, OH); PTT-A reagent (Diagnostica Stago, Asnieres-sur-Seine, France); argatroban (GlaxoSmithKline, Brentford, United Kingdom); polyphosphate (poly-P; 200-1300 units; provided by James Morrissey, University of Michigan); and horseradish peroxidase–conjugated antihemagglutinin immunoglobulin G (IgG; Invitrogen). Monoclonal IgGs 15H8 and 1B28,18 (link) are described.
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2

Ellagic Acid Extraction and Characterization

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Ellagic acid was purchased from Acros Organics (Belgium, WI, USA), with a purity of 97%. Diquat (average molecular weight: 344.05) was derived from Shanghai Fusheng Biotechnology Co., Ltd. (Shanghai, China). ML385 was derived from Glpbio (Montclair, CA, USA).
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3

Antioxidant Compounds Characterization

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Water was distilled and purified using a Stakpure GmpH water system (Niederahr, Germany). Gallic acid, ellagic acid, AlCl3, and Trolox were purchased from Acros Organics (Geel, Belgium). Chlorogenic acid was purchased from the HWI group (Rülzheim, Germany). Na2CO3 was obtained from Honeywell (Charlotte, NC, USA), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was obtained from Alfa Aesar (Kandel, Germany). Folin–Ciocalteu reagent was purchased from Fisher Scientific (Loughborough, UK). All solvents used were analytical or HPLC grade. Acetonitrile and formic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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4

Antioxidant Activity Evaluation of Styrene

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Styrene (99%, extra pure, stabilized), antioxidants (trolox (97%), rutin (97+%), ellagic acid (≥97%)), and polyvinylpyrrolidone (PVP, K-30, Mw 50,000) were purchased from Acros Organics (Geel, Belgium). The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical (95%) was bought from Alfa Aesar (Kandel, Germany). Potassium chloride (KCl), potassium peroxodisulphate (KPS), ethanol, and methanol were acquired from VWR (Debrecen, Hungary). Nitrogen (4.5) gas was obtained from Messer (Budapest, Hungary). All chemicals were used without further purification. Ultrapure water with a resistivity higher than 18.2 MΩ∙cm was obtained from a three-stage VWR Puranity TU+ (Debrecen, Hungary) device. The measurements were carried out at 25 °C and at pH 10 unless otherwise indicated.
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