Eos 77d

Powder XRD patterns of the as‐synthesized SAOCD were carried out on an XRD‐6100 (Shimadzu. Japan) X‐ray diffractometer with CuKα radiation (λ = 1.54178 Å). The morphology and element distribution of the samples were obtained by SEM (FEI Quanta 650 FEG) with energy dispersive X‐ray spectroscope fittings (EDAX ELEMENT). The TL curves were measured using a microcomputer thermoluminescence spectrometer (FJ427A1, Beijing Nuclear Instrument Factory) at a heating rate of 1 Κ s⁻¹. ML signals were collected in situ from a homemade tensile testing machine using a high‐throughput optical fiber. The ML spectra were recorded by a fluorescence spectrophotometer (Omni‐λ300i, Zolix Instruments Co., Ltd.) equipped with a CCD camera (iVac‐316, Edmund Optics Ltd.). The stress‐strain curve of SAOCD/PDMS elastomer was applied by a universal testing machine (Shimadzu AGS‐X‐500 N). The triboelectric potential was collected by using the electrostatic measuring probe (SK050, KEYENCE (Japan) Co., Ltd.) at a distance of 10 mm. The CL spectrum of SAOCD was detected on the modified Mp‐Micro‐S instrument attached to the SEM. High‐temperature environments were provided by the heating table (JF‐956A). All optical photos were taken by a digital camera (Canon EOS 77D).

Rats were anesthetized and perfused with normal saline (NS) and 4% paraformaldehyde via the ventricle. The prevascularized TENGs implanted subcutaneously and the bridge segment including nerve stumps on both sides were harvested. The bilateral gastrocnemius and anterior tibialis were removed and measured to calculate the wet weight ratio. The photos of implanted prevascularized TENGs and muscles were taken with a digital camera (EOS77D, Canon). The collected tissues were post-fixed for 6–8 ​h at 4 ​°C, frozen and cut into 12-μm-thick slices. The prevascularized TENGs both implanted and bridged were firstly transparented in gradient glycerol aqueous solutions from 50%, 75%, 85%–100% successively for 24 ​h, and photographed under the stereomicroscope (AZ100, Nikon). The frozen sections were then stained with hematoxylin-eosin (HE) and immunofluorescence staining to analyze angiogenesis, inflammation or nerve regeneration.

Specimens for this study were collected on Mount Jiulong, Zhejiang, China, in July 2019 by the authors. Adult crane flies were collected by insect net and at artificial light. Genitalic preparations of males were made by macerating the apical portion of the abdomen in cold 10% NaOH for 12–15 hours. Observations and illustrations were made using a ZEISS Stemi 2000-C stereomicroscope. Photographs were taken with a Canon EOS 77D digital camera through a macro lens. Type specimens of known Chinese species deposited in the National Museum of Natural History, Smithsonian Institution, Washington, DC, USA (USNM), the Natural History Museum, London, UK (NHM) and the Entomological Museum of China Agricultural University, Beijing, China (CAU) were examined. Type specimens of the new species were deposited in the Entomological Museum of Qingdao Agricultural University, Shandong, China (QAU).
The morphological terminology mainly follows McAlpine (1981) , and the venation is described after Alexander and Byers (1981) . Terminology of the male hypopygium follows Ribeiro (2006) (link). The following abbreviations are used: tg 9 = ninth tergite, tg 10 = tenth tergite, goncx = gonocoxite, c gonst = clasper of gonostylus, l gonst = lobe of gonostylus, aed = aedeagus, pm = paramere, cerc = cercus, hyp vlv = hypogynial valve, mm = millimeter.

Specimens for this study were collected from several localities in China by different entomologists between 2002–2016. Type specimens are deposited in the Entomological Museum of China Agricultural University, Beijing, China (CAU). Other studied specimens are deposited in Qingdao Agricultural University, Shandong, China (QAU). We also examined specimens from the National Museum of Natural History, Smithsonian Institution, Washington D.C., USA (USNM) and the Natural History Museum, London, UK (NHM) (Table 1). Genitalic preparations of males were made by macerating the apical portion of the abdomen in cold 10% NaOH for 12–15 hours. Observations and illustrations were made using a ZEISS Stemi 2000-C stereomicroscope. Photographs were taken with a Canon EOS 77D digital camera through a macro lens. Details of coloration were examined in specimens immersed in 75% C₂H₅OH.
The morphological terminology mainly follows McAlpine (1981) , and that for venation follows Alexander and Byers (1981) . The following abbreviations in figures are used: tg 9 = ninth tergite, tg 10 = tenth tergite, goncx = gonocoxite, o gonst = outer gonostylus, i gonst = inner gonostylus, aed = aedeagus, pm = paramere, cerc = cercus, hyp vlv = hypogynial valve.

All photos were taken using a Canon EOS 77D (Canon, Ōta, Tokyo, Japan) camera with a Dermlite Foto II Pro lens (3Gen Inc., San Juan Capistrano, CA, USA). The resolution of the images was 6000 × 4000 pixels with sRGB color space. All images have the same parameters: ISO 800, time of exposition 1/60 of a second. All lesions were photographed in the same exposition under non-polarized and polarized illumination. The applied lens enables shooting repeatable images on the same scale and the stable lighting condition, obtaining comparable photos of different lesion types. It also offers a built-in millimeter-scale enabling precise measuring of the lesion.

All photographs were taken using a Canon EOS 77D, Canon 60 mm f/2.8 EF-S USM Macro lens (Canon, Ōta, Tokyo, Japan) with Metz 15 MS-1 ring light (Metz, Markham, ON, Canada).
The same distance (45 cm) was achieved to take all of photos. An optical axis of the camera was kept perpendicular to the surface of the lesion. The focus plane was locked to 45 cm to obtain repeatability. Photographs during laser irradiation were taken in the same way as in the white light. Laser parameters were set to 0.8 W for single wavelengths (405, 450 nm), and 1.6 W for 405 + 450 nm. The optic fibre was equipped with a special diffuser of light which enables uniform irradiation of the surface. To achieve similar histogram filling, we used the following parameters of photographs: 405 nm—ISO 3200, f/9, time of exposure 1/90 s, 450 nm—ISO 3200, f/9, 1/250 s, 405 + 450 nm—ISO3200, f/9, 1/500 s. Examples of photographs taken in various wavelengths are shown in Figure 4.