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Anti hnf4a

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Anti-HNF4A is a laboratory reagent used for the detection and analysis of the HNF4A protein in biological samples. HNF4A is a transcription factor that plays a key role in the regulation of gene expression, particularly in the liver and pancreas. The Anti-HNF4A product provides a tool for researchers to investigate the expression and function of HNF4A in various biological systems.

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Lab products found in correlation

Anti sox9, by Merck Group (2 mentions) Anti cxcr4, by BD (1 mentions) Anti sox17, by R&D Systems (1 mentions) Cytofix cytoperm solution, by BD (1 mentions) Facscalibur instrument, by BD (1 mentions) Anti yap1, by Cell Signaling Technology (1 mentions) Anti brd4, by Fortis Life Sciences (1 mentions) Goat anti mouse hrp, by Bio-Rad (1 mentions) Normal rabbit mouse igg, by Santa Cruz Biotechnology (1 mentions) Anti h3k4me1, by Abcam (1 mentions) Anti total h3, by Abcam (1 mentions) Anti h3k122ac, by Abcam (1 mentions) Anti hnf4a, by Abcam (1 mentions) Anti h3k4me3, by Active Motif (1 mentions) Goat anti rabbit hrp, by Bio-Rad (1 mentions) Anti ki67, by Thermo Fisher Scientific (1 mentions) Anti tead4, by Santa Cruz Biotechnology (1 mentions) Anti yap1 63, by Santa Cruz Biotechnology (1 mentions) Anti tead4, by Aviva Systems Biology (1 mentions) Anti tead1, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

HNF4a (24 citations) Goat anti-Hnf4a (3 citations) Goat polyclonal anti-Hnf4a (2 citations)

4 protocols using anti hnf4a

1

Dissociation and Flow Cytometry Characterization of Cells

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Cells were dissociated for 10 min using 1× TrypLE Express (Thermo Fisher) and washed with phosphate-buffered saline (PBS) containing 1% foetal bovine serum (FBS). For intracellular marker staining, cells were fixed, permeabilized with BD Cytofix/Cytoperm solution (BD Biosciences), and stained with 1 μg of anti-SOX17 (R&D Systems), anti-ALB (Dako), and anti-HNF4A (Santa Cruz Biotechnology) antibodies. For surface marker staining, cells were fixed in 4% paraformaldehyde (Sigma-Aldrich) for 20 min at room temperature, washed with PBS containing 1% FBS and then incubated for 30 min at 4 °C with 1 μg of anti-CXCR4 (BD Biosciences) and anti-asialoglycoprotein receptor 1 (Santa Cruz Biotechnology) antibodies. Flow cytometry was performed using a BD FACSCalibur instrument (BD Biosciences).
Jo H.Y., Han H.W., Jung I., Ju J.H., Park S.J., Moon S., Geum D., Kim H., Park H.J., Kim S., Stacey G.N., Koo S.K., Park M.H, & Kim J.H. (2020). Development of genetic quality tests for good manufacturing practice-compliant induced pluripotent stem cells and their derivatives. Scientific Reports, 10, 3939.
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2

Investigating Transcriptional Regulation Mechanisms

Cited 1 time
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ChIP: anti-H3K27ac (Abcam, ab4729), anti-H3K4me3 (Active Motif, #39159), anti-H3K4me1 (Abcam, ab8895), anti-H3K122Ac (Abcam ab33309), anti-MycN262 (Santa Cruz, sc-764), anti-RNAPol2 N20-X (Santa Cruz, sc-899), anti-YAP1 63.7 (Santa Cruz, sc-101199), anti-TEAD4 (Aviva Systems Biology, ARP38276), anti-BRD4 (Bethyl, A301-985A100), anti-HNF4a (Abcam, ab41898). Normal rabbit/mouse IgG (Santa Cruz, sc-2027) was used as background control. Please note that the anti-TEAD4 (Aviva Systems Biology, ARP38276) was reported to recognize also TEAD1 and TEAD3 (33 (link)). Western-Blot: anti-YAP1 63.7 (Santa Cruz, sc 101199) and anti-TEAD4 (Santa Cruz, sc-101184); anti-HNF4a (Abcam, ab199431), anti-total H3 (Abcam, ab1791); anti-vinculin clone H (SigmaAldrich, V9131); anti-TEAD1 (Cell Signalling, #8526). Goat anti-rabbit HRP (Biorad, #1706515) and Goat anti-mouse HRP (Biorad, #1706516) were used as secondary antibodies. Immunohistochemistry: anti-YAP1 (Cell Signalling, #4911), anti-Ki67 (Thermo Scientific, #9106), anti-Sox9 (Millipore, #5535), anti-HNF4a (Santa Cruz, sc-8987 and Abcam, ab41898).
Biagioni F., Croci O., Sberna S., Donato E., Sabò A., Bisso A., Curti L., Chiesa A, & Campaner S. (2022). Decoding YAP dependent transcription in the liver. Nucleic Acids Research, 50(14), 7959-7971.
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3

Validation of NBCe1 and NBCe2 Antibodies

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The antibody to NBCe1 (Sigma WH0008671M1) has been well validated in human renal tissue in our previous studies in the RPT [17 (link)]. The NBCe2 antibody (Sigma HPA036621) used previously [17 (link)] and in the current studies was characterized in the Human Protein Atlas (http://www.proteinatlas.org/ENSG00000188687-SLC4A5/tissue/kidney#imid_20081509). We further verified its specificity using confocal imaging with dual staining for hRPTC-specific marker CD-13 (BD 347837, 1:500 dilution) or HNF4A (goat polyclonal anti-HNF4A, Santa Cruz sc-6556, 1:500). This was performed in cultured primary and immortalized hRPTCs, including overexpressed cell lines or those stably transfected with NBCe2-specific siRNA to knockdown SLC4A5, the NBCe2 gene.
Gildea J.J., Xu P., Kemp B.A., Carlson J.M., Tran H.T., Bigler Wang D., Langouët-Astrié C.J., McGrath H.E., Carey R.M., Jose P.A, & Felder R.A. (2018). Sodium bicarbonate cotransporter NBCe2 gene variants increase sodium and bicarbonate transport in human renal proximal tubule cells. PLoS ONE, 13(4), e0189464.
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4

Hepatocyte and Cholangiocyte Differentiation

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For hepatocyte differentiation, the collected hepatoblasts were plated on gelatin-coated (Sigma) 24-well dishes for 6 days. For cholangiocyte differentiation, the collected hepatoblasts were plated on Matrigel-coated (BD Bioscience) 24-well dishes for 10 days. Cells were cultured in DMEM/F12 (Gibco) medium supplemented with 10% FBS, 1× ITS-X (Invitrogen), 1× Penicillin-Streptomycin (Gibco), 25 ng/mL hEGF, 25 ng/mL hHGF, and 40 ng/mL dexamethasone (Sigma). For immunostaining, cells were stained with anti-SOX9 (Millipore, ab5535, 1:200), anti-HNF4A (Santa Cruz Biotechnology, sc-6556, 1:50), Alexa Fluor 488 donkey anti-goat IgG (Invitrogen, A11055, 1:1000), and Alexa Fluor 488 donkey anti-rabbit IgG (Invitrogen, A11008, 1:1000). DAPI (Sigma, D9564, 0.5 µg/mL) was used for nuclear staining. Images were acquired using an LSM 710 NLO and DuoScan System (Zeiss).
Wang X., Yang L., Wang Y.C., Xu Z.R., Feng Y., Zhang J., Wang Y, & Xu C.R. (2020). Comparative analysis of cell lineage differentiation during hepatogenesis in humans and mice at the single-cell transcriptome level. Cell Research, 30(12), 1109-1126.
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