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α pt202 y204erk 1 2

Manufactured by Cell Signaling Technology
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α-pT202/Y204Erk 1/2 is a lab equipment product that detects phosphorylation of Erk1/2 at threonine 202 and tyrosine 204. It is a specific antibody that can be used to identify the activation state of the Erk1/2 proteins.

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Lab products found in correlation

α gapdh, by Cell Signaling Technology (2 mentions) α actin, by Cell Signaling Technology (2 mentions) α erk1 2, by Abcam (2 mentions) α hk2, by Cell Signaling Technology (2 mentions) α hif1α, by Cell Signaling Technology (2 mentions) α tubulin, by Cell Signaling Technology (2 mentions) α drp1, by Abcam (2 mentions)

2 protocols using α pt202 y204erk 1 2

1

Western Blot Analysis of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole cell lysates were prepared in RIPA buffer and equivalent protein amounts (generally 50 μg) were resolved by SDS-Page. Gels were transferred to PVDF membranes and immunoblotted with the indicated antibodies. List of antibodies used in this study: α-Drp1 (Abcam), α-Erk1/2, α-pT202/Y204Erk 1/2, α-HK2, α-HIF1α, α-Tubulin, α-Actin, α-GAPDH (Cell Signaling Technologies).
Nagdas S., Kashatus J.A., Nascimento A., Hussain S.S., Trainor R.E., Pollock S.R., Adair S.J., Michaels A.D., Sesaki H., Stelow E.B., Bauer T.W, & Kashatus D.F. (2019). Drp1 Promotes KRas-Driven Metabolic Changes to Drive Pancreatic Tumor Growth. Cell reports, 28(7), 1845-1859.e5.
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2

Western Blot Analysis of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole cell lysates were prepared in RIPA buffer and equivalent protein amounts (generally 50 μg) were resolved by SDS-Page. Gels were transferred to PVDF membranes and immunoblotted with the indicated antibodies. List of antibodies used in this study: α-Drp1 (Abcam), α-Erk1/2, α-pT202/Y204Erk 1/2, α-HK2, α-HIF1α, α-Tubulin, α-Actin, α-GAPDH (Cell Signaling Technologies).
Nagdas S., Kashatus J.A., Nascimento A., Hussain S.S., Trainor R.E., Pollock S.R., Adair S.J., Michaels A.D., Sesaki H., Stelow E.B., Bauer T.W, & Kashatus D.F. (2019). Drp1 Promotes KRas-Driven Metabolic Changes to Drive Pancreatic Tumor Growth. Cell reports, 28(7), 1845-1859.e5.
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