Sirius red stain kit

Synovial tissues were collected after executing rats, and tissues were then fixed in 10% neutral formalin, embedded in paraffin, and cut into slices for routine HE staining. Sirius Red staining was carried out according to the instructions of the Sirius Red Stain Kit (Beyotime Biotechnology, Shanghai, China). Briefly, the tissue sections were dewaxed, dipped into water, stained with 1 g/l picric acid-Sirius Red at 37°C for 1 h, and then washed with water. The sections were mounted and viewed under a Leica DMI3000B microscope (Leica, Germany) with the use of a bright field.

Synovial tissues were fixed in 10% neutral formalin after rats were executed, embedded in paraffin, and cut into slices for routine staining. We followed Kenn's criteria in scoring and analyzing sections to evaluate synovitis [21 (link)].
Sirius Red staining was carried out according to the instructions of Sirius Red Stain kit (Beyotime Biotechnology, Shanghai, China). Sections were mounted and viewed under a Leica DMI3000B microscope (Leica, Germany), with the use of bright fields.

Synovial tissues were fixed in 10% neutral formalin after rats executed, embedded in paraffin, and cut into slices, for routine HE staining. Masson staining was performed in turn to potassium dichromate, ferryhematoxylin, bright spring red dye, phosphomolybdic acid, and aniline blue staining after paraffin section was prepared. Sirius Red staining was carried out according to the instructions of Sirius Red Stain Kit (Beyotime Biotechnology, Shanghai, China). Sections were mounted and viewed under a Leica DMI3000B microscope (Leica, Germany), with the use of bright field, or under a Nikon Eclipse E100 microscope (Nikon, Japan), with a polarized light field.