LC-MS-grade acetonitrile and ammonium acetate, as well as NH3, were obtained from VWR (Vienna, Austria). Analytical-grade acarbose, arabinose, erythritol, fructose, galactose, glucose, inositol, lactitol, lactose, maltitol, raffinose, rhamnose, ribose, sucrose, xylitol, potassium chloride, ammonium iodide and potassium bromide were purchased from Sigma Aldrich (Schnelldorf, Germany). Erythrose, isomaltulose, lyxose, maltose, maltotriose, mannitol, mannose, sorbitol, sorbose, xylose, sodium nitrate and sodium sulfate were purchased from VWR (Vienna, Austria). LC-MS-grade water (< 0.055 µS cm−1) from an ultrapure water purification system (Sartorius, Göttingen, Germany) was used for both elution and sample preparation. Food samples produced by various companies were obtained from local supermarkets. Sample matrices have been selected over a wide range of beverages and food to investigate the impact on sample preparation and robustness of the analytical method. Food and beverages were stored at the recommended temperature until analysis.
Maltose
Manufactured by Avantor
Sourced in Belgium, United States
Maltose is a disaccharide composed of two glucose molecules linked together. It is a common byproduct of the starch digestion process and is frequently used in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Sucrose, by Merck Group (2 mentions)
Sodium sulfate, by Avantor (1 mentions)
Inositol, by Merck Group (1 mentions)
Maltitol, by Merck Group (1 mentions)
Sodium nitrate, by Avantor (1 mentions)
Ribose, by Merck Group (1 mentions)
Ammonium iodide, by Merck Group (1 mentions)
Rhamnose, by Merck Group (1 mentions)
Arabinose, by Merck Group (1 mentions)
Ammonium acetate, by Avantor (1 mentions)
Xylitol, by Merck Group (1 mentions)
Mannitol, by Avantor (1 mentions)
Raffinose, by Merck Group (1 mentions)
Galactose, by Merck Group (1 mentions)
Potassium bromide, by Merck Group (1 mentions)
Xylose, by Avantor (1 mentions)
Lactitol, by Merck Group (1 mentions)
Lc ms grade acetonitrile, by Avantor (1 mentions)
Potassium chloride (kcl), by Merck Group (1 mentions)
Fructose, by Merck Group (1 mentions)
5 protocols using maltose
1
Comprehensive LC-MS Analytical Methodology
2
Electrochemical Analysis of Cocaine in Street Samples
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The cocaine·HCl standard was purchased from Lipomed (Arlesheim, Switzerland). Standards of phenacetin, diltiazem, lidocaine, procaine, hydroxyzine, benzocaine, paracetamol and myo-inositol were purchased from Sigma-Aldrich (Diegem, Belgium). Standards of benzoic acid and levamisole were purchased from Acros Organics (Geel, Belgium). Standards of caffeine, boric acid, glucose, maltose and starch were purchased from VWR Chemicals (Leuven, Belgium) and a standard of d -sorbitol was purchased from Merck Chemicals KGaA (Overijse, Belgium). Authentic cocaine street samples were obtained from the National Institute for Criminalistics and Criminology (Brussels, Belgium). Gelatine gel B was supplied by PB gelatins (United Kingdom). Carbon ItalSens IS-C Screen Printed Electrodes (SPE) were purchased from PalmSens (Utrecht, The Netherlands) and were used during all electrochemical measurements. The electrode surface area is 7.07 mm2. All laboratorium-based electrochemical measurements were performed using a Metrohm μAutolab III Potentiostat and NOVA 1.11 software.
3
Comparative Analysis of Carbohydrate Compounds
4
Purification and Cleavage of Fusion Proteins
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The hybrid protein and AβBP were cloned into pMAL-C5E MBP vector (New England Biolabs) with a cleavage site for 3C protease immediately after MBP. The fusion proteins were expressed in BL21 (DE3) E. coli, purified with amylose (New England Biolabs #E8022L) columns, eluted with maltose (VWR #1B1184), and dialyzed against 1X PBS. On the other hand, the MPDcontaining Tubby N-terminal was expressed and purified as described previously (Caberoy et al., 2010a) . The purified FLAG-tagged proteins were cleaved from MBP or Glutathione S-transferase (GST) using 3C protease (Genscript #Z03092-100) and their purity was analyzed by SDS-PAGE.
5
Enzymatic Glucose and Fructose Analysis
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As invertase standard α-glucosidase from Saccharomyces cerevisiae (EC 3.2.1.20, 125 U mg -1 protein, Sigma-Aldrich) was used.
Glucose, fructose, sucrose, maltose, p-nitrophenyl-α-D-glucopiranoside (pnf G, C 12 H 15 NO 8 ) and paranitrophenol (pnf, O 2 NC 6 H 4 OH) were purchased from VWR International LLC (Radnor, PA, USA).
Water purified with an ELGA Purelab Option DV 25 system (ELGA LabWater, Lane End, UK) was used.
All other chemicals were of analytical grade. The working electrolyte used for amperometric measurement was 0.1 M phosphate buffer (PBS).
Honey samples were purchased from a local shop, and were diluted to 1 m/V% concentration by the appropriate phosphate buffer solution.
The stock solutions of pnf G (10 mM) and the carbohydrates (25mM) were freshly prepared every working day.
The pnf standard samples (0.1 and 0.01 mM) were diluted from the stock solution (10 mM) with PBS before the measurement.
Glucose, fructose, sucrose, maltose, p-nitrophenyl-α-D-glucopiranoside (pnf G, C 12 H 15 NO 8 ) and paranitrophenol (pnf, O 2 NC 6 H 4 OH) were purchased from VWR International LLC (Radnor, PA, USA).
Water purified with an ELGA Purelab Option DV 25 system (ELGA LabWater, Lane End, UK) was used.
All other chemicals were of analytical grade. The working electrolyte used for amperometric measurement was 0.1 M phosphate buffer (PBS).
Honey samples were purchased from a local shop, and were diluted to 1 m/V% concentration by the appropriate phosphate buffer solution.
The stock solutions of pnf G (10 mM) and the carbohydrates (25mM) were freshly prepared every working day.
The pnf standard samples (0.1 and 0.01 mM) were diluted from the stock solution (10 mM) with PBS before the measurement.
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