Mouse gene mta 1.0 array

Neutrophils were treated with or without S1P, and total RNA was collected for further analysis. Converted RNA samples into individual cDNA libraries, and double-stranded cDNA was labeled and hybridized to an Affymetrix Mouse Gene MTA 1.0 array (Affymetrix, Santa Clara, CA, USA). To select the differentially expressed genes, we used threshold values of ≥2-fold change and corrected p value ≤0.05. The function and pathway of differentially expressed genes were explored by GO database. mRNA microarray experiments and technical assistance in bioinformatic analysis were handled by Capitalbio Technology (Beijing, China).

Briefly, samples (BMSCs treated with TGF-β1 with or without miR-26b-5p mimic) were used to synthesize double-stranded cDNA, and double-stranded cDNA was labeled and hybridized to an Affymetrix Mouse Gene MTA 1.0 array (Affymetrix, Santa Clara, CA, USA). To select the differentially expressed genes, we used threshold values of ≥1.3-fold change and corrected p value < 0.05. KEGG, Reactome, and GO analyses were performed to explore the function and pathways based on differentially expressed genes. mRNA microarray experiments and technical assistance in bioinformatic analysis were handled by Capitalbio Technology (Beijing, China).