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7 protocols using n hexane

1

Surface Modification of Polyester Fabrics

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Polyester fabrics (100% PET; 86 g/cm2) were purchased from the Aotai Textile Sales Company, China. DPOP and VTES were obtained from Shanghai Aladdin Biochemical Technology Co. Ltd., China. MTES and OTMS were supplied by Shanghai Aladdin Biochemical Technology Co. Ltd., China. Sodium hydroxide (NaOH) was obtained from Tianjin Beichenfangzheng Chemical Co. Ltd., China. Ethanol (AR), butanol, hexanol, acetone, THF, DMSO, TCM, toluene, n-hexane, and PE were supplied by Chengdu Kelong Chemical Co. Ltd., China. All materials were used without further treatment.
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2

Synthesis of Tailored Polymers via ATRP

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ε-Caprolactone (99%; Aladdin) was dehydrated by calcium hydride and filtrated by using microfilter (0.45 μm) before use. Poly(ethylene glycol) (Mn = 2 K; Aladdin) was dried by azeotropic distillation in the presence of toluene. Diphenyl phosphate (97%; Energy Chemical), cuprous bromide (99%; Aladdin), N,N’,N’,N”,N”- pentamethyldiethylenetriamine (PMDETA) (99%; Aladdin), 2-(dimethylamino)ethyl methacrylate (DMAEMA) (99%; Aladdin), α-bromo-γ-butyrolactone (αBrγBL) (98%; J&K Chemicals) were used without further purifications, toluene (AR; Chongqin Chuandong Chemical Group Co. Ltd.), ether (AR; Chongqing Chuandong Chemical Group Co. Ltd.), methylene dichloride (AR; Chengdu Jingshan Chemical Reagent Co. Ltd.), n-hexane (AR; Chengdu Kelong Chemical Reagent Factory) and basic alumina (100–200 mesh; Aladdin) were used as received.
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3

Detailed Reagent Procurement Protocol

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Vanillin was purchased Shanghai Sangon Biological Engineering Technology (Shanghai, China). o-Toluidine and N, N-dimethylformamide were purchased from Shanghai Aladdin Bio-Chem Technology Co., LTD (Shanghai, China). Sodium citrate tribasic dihydrate and Tris were purchased from Sigma-Aldrich (Steinheim, Germany). Glycine was purchased from GenStar Biosolutions Co., Ltd. (Beijing, China). Citric acid, disodium hydrogen, HCl, NaOH, Na2HPO4 and acetic acid were purchased from Guangzhou Chemical Reagent Factory (Guangzhou, China). Acetonitrile was purchased from Tianjin Damao Chemical Reagent Factory (Tianjin, China). n-Hexane was purchased from Chengdu Kelong Chemical Reagent Factory (Chengdu, China). Other chemicals were purchased from standard commercial sources and were of analytical grade purity.
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4

Polysulfone-Based Membrane Fabrication

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The polysulfone (PSF, Udel® p-3500, Mn = 22 000 g mol−1) was purchased from Solvay Co. Ltd. 1,3,5-Benzenetricarboxylic chloride (TMC, purity > 98%), m-phenylenediamine (MPD, purity > 99%), l-arginine (Arg, purity > 99%), bovine serum albumin (BSA, purity > 99%) and dodecyltrimethyl ammonium bromide (DTAB, purity > 99%) were purchased from Shanghai Aladdin Biochemical Technology Co. Ltd. Other reagents including n-hexane (purity > 97%), N,N-dimethylformamide (DMF, purity > 99.5%) and sodium chloride (NaCl, purity > 99.5%) were purchased from Chengdu Kelong Chemical Reagent Co. Ltd. Distilled water in all experiments was made by water distiller.
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5

Hydroxyapatite Porous Ceramics Fabrication

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The HAPPs were prepared by using paraffin spheres with diameters of about 3 mm and about 0.8 mm as the pore-forming agents, respectively, according to the preparation method of our earlier publication [30 (link)]. Briefly, HA powders were dispersed in a chitin-lithium chloride solution in dimethylformamide (all from Chengdu Kelong Chemical Co., Ltd., China) to form a suspension. Subsequently, the melting paraffin was dropped into a stirred polyvinyl alcohol (PVA, Chengdu Kelong Chemical Co., Ltd., China) to form the paraffin spheres. These spheres were then accumulated in a cylindrical mold and immersed in xylene (Chengdu Kelong Chemical Co., Ltd., China) and given pressure for a while to control the inter-sphere connectivity. The HA suspension was infused and gelled with water in air. Paraffin was wiped off by immersing in n-hexane (Chengdu Kelong Chemical Co., Ltd., China). The initial scaffolds were sintered into ceramic objects (Figure 2). The two macro-pore sizes of HAPPs were labeled L-HAPPs and S-HAPPs, respectively.
All the scaffolds were sealed in vacuum packages and sterilized with ethylene oxide for subsequent implantation.
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6

Collagen-targeting Nanomedicine Synthesis

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Acetylacetonate iron (99.9%), oleic acid (90%), oleylamine (70%), 1,2-hexadecanediol (97%), diphenyl ether (99%), monomethyl polyethylene glycol (mPEG-OH, molecular weight (Mw): 550 Da), and dopamine, were purchased from Sigma-Aldrich LLC. (St. Louis, MO, USA). Maleimide polyethylene glycol succinimidyl carboxymethyl ester (MAL-PEG2000-SCM) was purchased from Beijing Jenkem Technology Co., Ltd. (Beijing, China). Triethylamine was purchased from Beijing KeyKem Technology Co., Ltd. (Beijing, China). Tetrahydrofuran, acetone, n-hexane, anhydrous ethanol and other organic solvents were purchased from Chengdu Kelong Chemical Reagent Factory (Chengdu, China) and used directly. Peptide WYRGRL targeting cartilage matrix component collagen type II α1 was provided by ChinaPeptides Co. Ltd (Shanghai, China), which has been acetylated with a cysteine at the carboxy (C) terminus: Ac-WYRGRLC(Mw: 995.13, Purity: 97.43%, Supplementary Fig. S1). Papain: Molecular weight 23 406 Da, 10 units/mg, lyophilized powder, CAS number 9001-73-4, was from Sigma-Aldrich LLC. (St. Louis, MO, USA). Six-month-old New Zealand white rabbits with a body weight of 3.0–3.5 kg were purchased from Sichuan Dossy Experimental Animal Co., Ltd. (Chengdu, China).
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7

Superhydrophobic Surfaces for Oil-Water Separation

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Cornstarch (amylose content, ∼25%) was obtained from Xi'an Xiadian Corn Development Co., Ltd (Shanxi, China). Concentrated sulfuric acid (98%), phosphoric acid (85%), n-hexane (AR) were purchased from Chengdu KeLong Chemical Co., Ltd (Sichuan, China). Nickel foam (thickness 1.5 mm, surface density 350 ± 20 g m−2, PPI 110) was bought from Changsha Liyuan New Material Co., Ltd (Hunan, China). Hexamethyl disilylamine (HMDS) was supplied by Aladdin Industrial Corporation (Shanghai, China). In order to clearly demonstrate the oil/water separation, the distilled water used for oil/water separation was dyed by methyl orange (Chengdu KeLong Chemical Co., Ltd, Sichuan, China).
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