into a nonluminescent cell mounted
in a home-built liquid-nitrogen-cooled dewar whose bottom was made
of quartz. The samples were cooled to 77 K to increase the signal
intensity. Dried air was blown over the outside of the quartz windows
to avoid ice formation. The excitation pulse used was the fourth harmonic
generation of a Nd/YAG laser (Quanta Ray, Spectra Physics) coupled
with an optical parametric oscillator unit (Versa Scan and UV Scan)
to convert the wavelength of the laser light to 394 nm. The fluorescence
of the sample was collected at a right angle to the excitation beam
and directed via two lenses into the entrance slit of the spectrometer
(Shamrock RS 303i, 300 lines/mm, Andor Technology). The optically
triggered signals from the PIN photodiode to the controller in the
PC software were adjusted through a delay generator (DG535, Stanford
Research Inc.). The resultant spectra were recorded with a time-gated
ICCD camera (iStar, Andor Technology). The gate width was 25, 50,
or 100 μs, and the delay time after the excitation laser pulse
was 10 μs. Lifetime data were analyzed using the Origin (Light
Stone) software. Details on the detection system can be found in recent
refs.20 (link),22 (link)