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Quanta ray

Manufactured by Spectra-Physics
Sourced in United States, United Kingdom

The Quanta Ray is a pulsed Nd:YAG laser system designed for a wide range of laser spectroscopy and other research applications. It features a high-energy output, excellent beam quality, and flexible repetition rates. The core function of the Quanta Ray is to generate high-energy, short-duration laser pulses for use in various scientific and industrial settings.

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8 protocols using quanta ray

1

Time-Resolved Fluorescence Spectroscopy

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All samples were placed
into a nonluminescent cell mounted
in a home-built liquid-nitrogen-cooled dewar whose bottom was made
of quartz. The samples were cooled to 77 K to increase the signal
intensity. Dried air was blown over the outside of the quartz windows
to avoid ice formation. The excitation pulse used was the fourth harmonic
generation of a Nd/YAG laser (Quanta Ray, Spectra Physics) coupled
with an optical parametric oscillator unit (Versa Scan and UV Scan)
to convert the wavelength of the laser light to 394 nm. The fluorescence
of the sample was collected at a right angle to the excitation beam
and directed via two lenses into the entrance slit of the spectrometer
(Shamrock RS 303i, 300 lines/mm, Andor Technology). The optically
triggered signals from the PIN photodiode to the controller in the
PC software were adjusted through a delay generator (DG535, Stanford
Research Inc.). The resultant spectra were recorded with a time-gated
ICCD camera (iStar, Andor Technology). The gate width was 25, 50,
or 100 μs, and the delay time after the excitation laser pulse
was 10 μs. Lifetime data were analyzed using the Origin (Light
Stone) software. Details on the detection system can be found in recent
refs.20 (link),22 (link)
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2

Optical characterization of chiral plasmonic arrays

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We have used a custom-made polarimeter that measures the reflected light from our samples. It uses a tungsten halogen light source (Thorlabs), Glan-Thompson polarizers (Thorlabs) and a × 10 objective (Olympus). The samples are positioned with the help of a camera (Thorlabs, DCC1645C) and the spectrum is measured using a compact spectrometer (Ocean optics USB4000). Using Stokes methods, we can measure the intensity of light at four angles of the analyser and calculate the optical rotation dispersion of our chiral plasmonic arrays.
A nanosecond (8 ns)-pulsed Nd:YAG laser (Spectra Physics Quanta Ray) operating at a 10-Hz repetition rate was used for the irradiation. The sample was irradiated at normal incidence S-polarized light, using an unfocussed beam with an area of 1 cm2, for 1 min. Fluence was varied where stated. For all protein experiments, the fluence used was 15 mJ cm−2.
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3

Laser Flash Photolysis of CO-Ferrous Hemoglobin

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Laser flash photolysis (LFP) experiments were performed at 20°C using a laser photolysis system (Edinburgh Instruments LP920, UK) equipped with a frequency-doubled, Q-switched Nd:YAG laser (Quanta-Ray, Spectra Physics). The carbon monoxide (CO)–ferrous Hb complexes were prepared in sealed 4 × 10 mm quartz cuvettes with 1 ml of 100 mM potassium phosphate buffer (pH 7.0) containing 1 mM EDTA. In the case of LjGlb1-1 this buffer was supplemented with 200 mM NaCl to improve protein stability. The buffer was equilibrated with mixtures of CO and N2 in different ratios to obtain CO concentrations of 50–800 μM by using a gas mixer (High-Tech System; Bronkhorst, The Netherlands). Saturated sodium dithionite solution (10 μl) was added and the protein was injected to a final concentration of ∼4 μM. Formation of the CO–ferrous Hb complex was verified by UV/visible absorption spectroscopy. Recombination of the photo-dissociated CO-ligand was monitored at 417 nm.
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4

Laser-Assisted Wastewater Treatment

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Laser experiments were performed on 4 ml aliquots of IBP spiked (10 mg L -1 ), double distilled water or filtered effluent wastewater. Samples were placed inside 1 cm 2 base UV fused quartz cuvettes (CV10Q3500S, Thorlabs Inc., Newton, NJ, USA) and irradiated with a pulsed solid-state laser (Quanta Ray, Spectra-Physics, Newport Corp., Irvine, CA, USA). The laser emission was set at its fourth harmonic (=266 nm) with a pulse width of 4-5 ns and a pulse repetition frequency of 50 Hz. The nominal laser emission power was set at 0.9 W and the area covered by the beam was set at ca. 1 cm 2 . In consequence, the pulse fluence is ca. 18 mJ cm -2 and the pulse irradiance ca. 4 MW cm -2 . The laser beam was reflected on a narrowband laser line mirror (Y4-1025-45-UNP, CVI Laser Optics, Albuquerque, NM, USA) mounted onto a 45º round mount (Thorlabs Inc.), in order to vertically irradiate the cuvette contents from its top entry (Fig. 1). The laser power was measured employing a FieldMax II TO (Coherent Inc., Santa Clara, CA, USA) power meter equipped with a PowerMax PM150 sensor and placed at the same geometrical point as the quartz cuvettes.
It was observed during the treatment that the laser beam irradiated a complete, vertical liquid column, from the upper liquid meniscus to the bottom, inner base surface of the cuvette.
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5

Shadowgraphy of Laser-Produced Plasma

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Shadowgraphy used a second-harmonic probe beam from an Nd:YAG laser (Spectra Physics Quanta-Ray, 10 mJ, 10 ns pulse duration, 10 Hz repetition rate). The 8-mm diameter probe beam was incident onto a 1280 × 960 pixel CMOS detector (Mightex CGE-B013-U, 3.75 m pixel width). A laser-line filter was coupled to the detector in order to attenuate plasma emission. The probe was synchronized to every eighth pulse from the 80-Hz Lambda Cubed laser system. Images track the evolution of the shock front formed by the filament-target interaction at 100-ns increments following the onset of plasma formation up to 1 μs.
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6

Laser-Assisted Nanoparticle Synthesis

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A schematic illustration of the experimental set-up is shown in Figure 1. The second harmonic wavelength (532 nm) of a pulsed Q-switched Nd: YAG laser (Quanta Ray, Spectra-Physics) (Spectra-physics, Santa Clara, CA, USA) was focused on the sample using a lens (Thorlabs LB5284, CaF2 Bi-Convex Lens, f = 50.0 mm, uncoated) (Thorlabs, Newton, NJ, USA). The laser pulses had a repetition rate of 10 Hz with a duration of 6 ns. The laser output power was set to 0.1 W, which corresponds to a fluence of 0.02 J/cm2. To ensure stability, laser power was monitored throughout the experiment using a thermal power sensor (Surface Absorber, S350C, 0.19–1.1 μm and 10.6 μm, 40 W) (Thorlabs, Newton, NJ, USA) connected to a power meter (Thorlabs, PM 100A). As soon as the samples were prepared, as explained in Section 2.1, they were irradiated immediately in order to start the chemical reduction process and the laser irradiation simultaneously. Magnetic stirring of the sample was maintained during the irradiation process to allow homogeneous irradiation of the whole sample. The experiment was carried out at room temperature.
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7

Transient Absorption Spectroscopy of Photophysical Processes

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The transient absorption setup used as excitation source was the fourth harmonic of a nanosecond Nd:YAG laser (Quanta Ray, Spectra-Physics, Santa Clara, CA, USA). The excited area at the surface of the sample was 0.6 × 1.0 cm2. The analyzing beam, orthogonal to the exciting beam, was provided by a 150 W Xe-arc lamp (OSRAM XBO, Munich, Germany); its optical path length through the sample was 1 cm while its thickness was limited to 0.1 cm in order to use the most homogeneous part of the light. The probed volume was located in the very first 0.1 cm part of the cell along the propagation of the exciting laser beam delimited by four dedicated slits. Then, the analyzing light was dispersed in a SPEX 270M monochromator (Horiba Jobin-Yvon, Longjumeau, France), detected by a Hamamatsu R928 photomultiplier (Hamamatsu Photonics France, Massy, France) and recorded by a Waverunner 6084 oscilloscope (Lecroy, Teledyn Lecroy, Courtaboeuf, France). For measurements on the sub-microsecond scale, the Xe-arc lamp was intensified via an electric discharge (Applied Photophysics, Leatherhead, UK). Transient absorption spectra were recorded using a wavelength-by-wavelength approach. The excitation rate was 0.2 Hz. The incident pulse energy at the surface of the sample was measured using a NIST traceable pyroelectric sensor (Nova2/PE25, Ophir Spiricon Europe GmbH, Darmstadt, Germany).
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8

Measuring CO and O2 Binding Kinetics

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Laser flash photolysis experiments were performed to measure the CO and O 2 binding kinetics at 20 °C using a laser photolysis system (Edinburgh Instruments LP920, UK) equipped with a frequency-doubled, Q-switched Nd:YAG laser (Quanta-Ray, Spectra Physics). A laser flash of 532 nm was used.
The carbon monoxide (CO)-ferrous Hb complexes were prepared in sealed 4 × 10 mm quartz cuvettes with 1 ml of 100 mM potassium phosphate buffer (pH 7.0) containing 1 mM EDTA. The buffer was equilibrated with mixtures of CO and N 2 in different ratios to obtain CO concentrations of 50-800 µM by using a gas mixer (HighTech System; Downloaded by SYRACUSE UNIVERSITY from www.liebertpub.com at 10/21/19. For personal use only.
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