Bi3730xl sequenator

Retsch MM400 Ball Mill (Laichi co., Berlin, Germany); PTC200PCR (Bio‐Rad, Berkeley, CA, USA); GelDox XR gel imaging system (Bio‐Rad, USA); DYY‐8C Electrophoretic apparatus (Beijing Liuyi Instrument, China); BI3730XL sequenator (Applied Biosystems, Foster City, CA, USA); KRQ‐300P manual climatic box (ChongQing YinHe Test Instrument, China); Plant DNA Extraction Kit (TIANGEN Biotech Co., Chengdu, China); 2 × Taq PCR Master Mix (TIANGEN Biotech Co.,); primer was compounded by Sangon Co., China. Shimadzu; DM4000M microscope (Leica, Frankfurt, Germany); TruSeq^® DNA PCR‐Free Sample Preparation Kit (Illumina Co., San Diego, CA, USA); QIAquick gum recovery kit (QIAGEN, Duesseldorf, Germany); Phusion^® High‐Fidelity PCR Master Mix with GC Buffer (New England Biolabs, USA); High‐efficient enzyme (New England Biolabs, Boston, MA, USA).

They were as follows: Retsch MM400 Ball Mill(Laichi co., Germany); PTC200PCR(BIO-Rad, American); GelDox XR gel imaging system (BIO-Rad, American); DYY-8C Electrophoretic apparatus (Beijing Liuyi Instrument, China); BI3730XL sequenator (Applied Bio-systems, American); KRQ-300P manual climatic box (ChongQing YinBe Test Instrument, China); Plant DNA Extraction Kit (Tiangen Biotech Co., China); 2×Taq PCR MasterMix (Tiangen Biotech Co., China); primer was compounded by Sangon Co., China. Shimadzu; LC-20AT HPLC (Shimadzu Co., Japan); DM4000M microscope (Leica, Germany); Agilent 8453 ultraviolet and visible spectrophotometer (Agilent Technologies Co., American); hesperidin (MUST-12041206); narirutin (MUST-14081915); hesperetin (MUST-14100914); naringenin (MUST-14112310), four reference substance were bought from Chengdu Mansite biological technology co. LTD, purity>98.0%. The other reagents were analytically pure.