The largest database of trusted experimental protocols

5 protocols using ethanol special grade

1

Phytochemical Compound Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Piceatannol (>98.0%), resveratrol (>99.0%), rhapontigenin (>98.0%), isorhapontigenin (>96.0%), piceid (>95.0%), pinostilbene (>97.0%), caffeic acid (>98.0%), and p-coumaric acid (>98.0%) were obtained from Tokyo Chemical Industry (Tokyo, Japan). Gnetin C (>97.0%), and special grade ethanol (>99.5%) were obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Astringin was obtained from Sequoia Research Products limited company (Pangbourne, United Kingdom). Epicatechin (>90.0%) was obtained from Sigma-Aldrich Japan (Tokyo, Japan). LC-MS grade acetonitrile (>99.9%), LC-MS grade distilled water, and HPLC grade formic acid (>98.0%) were obtained from Kanto Chemical Co., Inc. (Tokyo, Japan).
+ Open protocol
+ Expand
2

Okinawan Vegetable Polyphenol Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols
Freeze-dried hosoba-wadan and nishi-yomogi cultivated
at Okinawa Prefecture Agricultural Research Center, Japan was extracted
twice (50 °C, 1 h) with methanol, and the solvent was evaporated. The polyphenol contents of
Okinawan vegetable methanol extracts were approximately 5 mg/g for
hosoba-wadan and 20–30 mg/g for nishi-yomogi. To adjust a concentration
of 1 mg/mL for the in vitro study, extracts were resolved with an
appropriate volume of special grade ethanol (99.5%, Wako Pure Chemical
Industries, Ltd. Osaka, Japan).
+ Open protocol
+ Expand
3

Ethanol and Ethylene Glycol Purification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ethanol (special grade) and ethylene glycol (special grade) were purchase from Wako Pure Chemical Industries (Osaka, Japan) and used without further purification.
+ Open protocol
+ Expand
4

Skin Permeability of Anti-Inflammatory Drugs

Check if the same lab product or an alternative is used in the 5 most similar protocols
FLU was purchased from Tokyo Kasei Kogyo Co., Ltd. (Tokyo, Japan). Etodolac, IDM, loxoprofen sodium, naproxen, piroxicam, LDC (for biochemistry), and ethanol (special grade) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). As the base for the skin permeability test, a gel base prepared by hydroxypropyl methylcellulose (HPMC, ~15 mPa·S, 2% in H2O (25 °C), Sigma-Aldrich Japan K.K., Tokyo, Japan) to 2 w/w% and white petrolatum (JP grade, Yoshida Pharmaceutical Co., Ltd., Tokyo, Japan) were used. A Franz-type diffusion cell (Osawa Shokai Co. Ltd., Tokyo, Japan) was used. Phosphate buffer solution (PBS, pH 7.4) was prepared from one tablet of phosphate buffered saline (PBS) (Sigma-Aldrich Japan, Tokyo, Japan) dissolved in 200 mL water. All other reagents used were commercially available special-grade products.
+ Open protocol
+ Expand
5

Cold-Cure Acrylic Resin Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
A multi-purpose cold-cure acrylic resin (PROCAST DSP clear shade, GC, Tokyo, Japan) was used, which consisted of a liquid component (MMA with a small amount of accelerator) and a powder component (fine PMMA spheres with a small amount of initiator on them). In the following sections, these liquid and powder components are referred to as MMA and PMMA, respectively. Ethanol (special grade, Wako, Osaka, Japan) was used as an MMA-compatible solvent.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!