Compounds were purchased from Sigma-Aldrich (Buchs, Switzerland) except for Sazetidine A dihydrochloride, NS9283 and dihydro-β-erythroidine hydrobromide that came from Tocris (Tocris bioscience, a Bio-Techne brand, Switzerland). The modulatory compounds were prepared as 20 mM stock solutions in dimethyl sulfoxide (DMSO) and were dissolved in oocyte Ringer’s OR2 medium (see below), resulting in a maximal final DMSO concentration of 0.5% when compounds were tested at 100 μM. ACh was obtained from Sigma-Aldrich and prepared as stock solution at 100 mM in ddH2O.
Ns9283
Manufactured by Bio-Techne
Sourced in Switzerland
NS9283 is a Biotinylated Antibody from Bio-Techne. It is a detection reagent used in immunoassay applications.
Automatically generated - may contain errors
Lab products found in correlation
Dihydro β erythroidine hydrobromide, by Bio-Techne (1 mentions)
Sazetidine a dihydrochloride, by Bio-Techne (1 mentions)
Scopolamine hydrobromide, by Bio-Techne (1 mentions)
Epibatidine, by Bio-Techne (1 mentions)
4 diphenylacetoxy n methylpiperidine methiodide 4 damp, by Cayman Chemical (1 mentions)
Nicotine, by Fujifilm (1 mentions)
Methyllycaconitine citrate mla, by Merck Group (1 mentions)
Dihydro β erythroidine hydrobromide dhβe, by Bio-Techne (1 mentions)
Nicotine, by Thermo Fisher Scientific (1 mentions)
Tetrodotoxin ttx, by Fujifilm (1 mentions)
2 protocols using ns9283
1
Modulatory Compounds for Oocyte Assays
2
Pharmacological Reagents for Nicotinic and Muscarinic Receptor Studies
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ACh, methyllycaconitine citrate (MLA), and 1-(4-methoxybenzyl)-4-oxo-1,4-dihydroquinoline-3-carboxylic acid (BQCA) were purchased from Sigma-Aldrich (St. Louis, MO). (-)-Nicotine, mecamylamine hydrochloride, and tetrodotoxin (TTX) were obtained from Wako (Osaka, Japan). NS 9283, (±)-epibatidine, 4-(4-bromophenyl)-3a,4,5,9btetrahydro-3H-cyclopenta[c]quinolone-8-sulfonamide (4BP-TQS), scopolamine hydrobromide, and dihydro-βerythroidine hydrobromide (DHβE) were purchased from Tocris Bioscience (Bristol, UK). VU 0255035 was obtained from Abcam (Cambridge, UK). (+)-Muscarine iodide was purchased from Toronto Research Chemicals (Toronto, Canada). 4-Diphenylacetoxy-N-methyl-piperidine methiodide (4-DAMP) was obtained from Cayman Chemical (Ann Arbor, MI). ACh, MLA, (-)-Nicotine, mecamylamine hydrochloride, DHβE, (+)-muscarine iodide, scopolamine hydrobromide, and TTX were dissolved in distilled water (Thermo Fisher Scientific, Waltham, MA). 4BP-TQS, NS 9283, (±)-epibatidine, BQCA, VU 0255035, and 4-DAMP were dissolved in DMSO (Wako).
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