Blood samples were acquired by either tail bleed or terminal cardiac puncture. Red blood cells and platelets were cleared using in RBC lysis buffer (BD PharmLyse 555899) and Hanks Balanced Salt Solution (Gibco 14185). Dead cells were eliminated using Live-Dead Fixable Yellow (0.55 μL/mL; Thermo-Fisher L34959). Prior to surface staining, Fc receptors were blocked with TruStain fcX anti-mouse CD16/32 antibody (10 μg/mL; Biolegend; 101320). Cells were stained with the following antibodies CD45 (5 μg/mL; eBiosciences; clone: 30-F11), CD11b (2.5 μg/mL; eBiosciences; clone: M1/70), Ly6C (2.5 μg/mL; Biolegend, clone: HK1.4), Ly6G (2.5 μg/uL; Biolegend; clone: 1A8), and CD115 (0.6 μg/mL; eBiosciences; clone: AFS98). OneComp ebeads (50 μL/sample; eBiosciences; 01-1111-42) were used for single stain controls and all experiments used fluorescent minus one (FMO) controls for each marker. All samples were run on a Beckman Coulter CyAn ADP LX flow cytometer equipped with 405-nm, 488-nm, and 633-nm lasers and analyzed using FlowJo Version 10 software.
Cyan adp lx flow cytometer
Manufactured by FlowJo
The CyAn ADP LX flow cytometer is a versatile instrument designed for high-performance flow cytometry applications. It features a compact and modular design, allowing for the analysis of a wide range of sample types. The CyAn ADP LX is capable of detecting and analyzing multiple parameters simultaneously, making it a reliable tool for various research and clinical applications.
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2 protocols using cyan adp lx flow cytometer
1
Murine Myeloid Cell Immunophenotyping
2
Murine Myeloid Cell Immunophenotyping
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Blood samples were acquired by either tail bleed or terminal cardiac puncture. Red blood cells and platelets were cleared using in RBC lysis buffer (BD PharmLyse 555899) and Hanks Balanced Salt Solution (Gibco 14185). Dead cells were eliminated using Live-Dead Fixable Yellow (0.55 μL/mL; Thermo-Fisher L34959). Prior to surface staining, Fc receptors were blocked with TruStain fcX anti-mouse CD16/32 antibody (10 μg/mL; Biolegend; 101320). Cells were stained with the following antibodies CD45 (5 μg/mL; eBiosciences; clone: 30-F11), CD11b (2.5 μg/mL; eBiosciences; clone: M1/70), Ly6C (2.5 μg/mL; Biolegend, clone: HK1.4), Ly6G (2.5 μg/uL; Biolegend; clone: 1A8), and CD115 (0.6 μg/mL; eBiosciences; clone: AFS98). OneComp ebeads (50 μL/sample; eBiosciences; 01-1111-42) were used for single stain controls and all experiments used fluorescent minus one (FMO) controls for each marker. All samples were run on a Beckman Coulter CyAn ADP LX flow cytometer equipped with 405-nm, 488-nm, and 633-nm lasers and analyzed using FlowJo Version 10 software.
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