Autoflex speed tof tof instrument

All NMR spectroscopy were measured on a Bruker 500 (500 MHz) spectrometer (Bruker, Karlsruh, Germany) for ¹³C and ¹H. MALDI-TOF MS was carried out on an autoflex speed TOF/TOF instrument (Bruker, Karlsruhe, Germany). FTIR spectroscopy (Perkin Elmer, Boston, MA, USA) was performed on a Spectrum 100 instrument. UV–vis spectroscopy data were acquired on Lamda 950 instrument (Thermo Fisher, Waltham, MA, USA). X-ray diffraction (XRD) measurements were performed on a D8 Advance X-ray diffractometer (Bruker, Karlsruhe, Germany) with a scanning speed of 6° min⁻¹ over the range 20–70° (2θ) with Cu Kα radiation (k = 1.54 Å) as the incident beam at a generator voltage of 40 kV and a generator current of 50 mA. Scanning electron microscopy (SEM, FEI, Portland, OR, USA) images were measured on a FEI Nova NanoSEM 450 instrument. Transmission electron microscopy (TEM, FEI, Portland, OR, USA) images were acquired using a FEI Tecnai G2 F20 S-TWIN instrument operated at 200 kV. Thermogravimetric analysis (TGA, TA, New Castle, DE, USA) measurements were performed on a Discovery TGA550 instrument. X-ray photoemission spectroscopy (XPS, Shimadzu, Kyoto, Japan) were performed on an AXIS UltraDLD instrument. Solid-state NMR spectra measurements were performed on an AVANCE NEO SK2-2-12a spectrometer operating (Bruker, Karlsruhe, Germany) at 600.13 MHz for ¹³C NMR.

Propionic acid (≥99.5%), pyrrole (99.0%), ethanol (C₂H₅OH, ≥99.8%), dichloromethane (CH₂Cl₂, ≥99.9%), ABDA (≥95.0%), MB (≥98.0%), and NADH (98.0%) were purchased from Aladdin Biochemical Technology Co., Ltd., Shanghai, China, Sulfuric acid (H₂SO₄, ≥96.0%), fuming Sulfuric acid (20%), p-methoxy benzaldehyde (≥99.0%), silver nitrate (AgNO₃, ≥99.9%), sodium carbonate (Na₂CO₃, ≥99.8%), sodium hydroxide (NaOH, ≥99.9%), Luria-Bertani (LB) agar, and LB broth were purchased from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China, The experimental consumables were mostly purchased from Corning (Corning, NY, USA) or BioFil (JET, Guangzhou, China).
The MALDI-TOF mass spectra were measured using an Autoflex Speed TOF/TOF instrument (Bruker, Karlsruhe, Germany) under the positive charge mode. Proton NMR (¹H NMR) spectra were acquired using an AVANCE Ⅲ HD 500 instrument (Bruker, Karlsruhe, Germany). FT-IR spectra were recorded on a Nicolet 6700 spectrometer (Thermo Fisher, MA, USA) in the range of 400–4000 cm⁻¹ using KBr tablets. XPS profiles were obtained using an XPS system (AXIS Ultra DLD, Kratos, Manchester, UK) to investigate the chemical state of the samples. The UV-vis absorption spectra were recorded on a Shimadzu UV-1800 spectrophotometer (Kyoto, Japan). The fluorescence emission spectra were detected using an FLS 1000 system (Edinburgh Instruments, Edinburgh, UK).