Daf fm staining

Manufactured by Thermo Fisher Scientific

DAF-FM is a fluorescent dye used in the detection and measurement of nitric oxide (NO) in biological samples. It binds to NO, producing a fluorescent signal that can be detected using appropriate excitation and emission wavelengths. The core function of DAF-FM is to provide a method for the quantitative analysis of NO levels in cells, tissues, or other biological systems.

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Lab products found in correlation

Brefeldin a, by Thermo Fisher Scientific (2 mentions) Fixation and intracellular staining permeabilization wash buffers for cytoplasmic, by Sony (2 mentions) Foxp3 transcription factor staining buffer set for nuclear proteins, by Thermo Fisher Scientific (2 mentions)

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DAF-FM (93 citations)

2 protocols using daf fm staining

Multiparametric Flow Cytometry Staining

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Cells were stained with primary and secondary antibodies in pre-determined dilutions at 4 °C for 15 min (see Supplementary Table 3). Intracellular antigens were stained after cell surface antigens, using Sony’s Fixation and Intracellular Staining Permeabilization Wash Buffers for cytoplasmic, (Sony, 2704005, 2705010), and eBioscience’s Foxp3/Transcription Factor Staining Buffer Set for nuclear proteins (Thermo, 00-5523-00). Cytokines and secretory molecules were analyzed after Brefeldin A treatment (eBioscience, 00-4506-51); 5 µg/ml for 2 h at 37 °C before staining. Intracellular nitric oxide (NO) levels were determined using DAF-FM staining (Thermo Fisher, D23844), as recommended by the manufacturer.

Érsek B., Silló P., Cakir U., Molnár V., Bencsik A., Mayer B., Mezey E., Kárpáti S., Pós Z, & Németh K. (2020). Melanoma-associated fibroblasts impair CD8+ T cell function and modify expression of immune checkpoint regulators via increased arginase activity. Cellular and Molecular Life Sciences, 78(2), 661-673.

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Comprehensive Cell Immunophenotyping Protocol

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Cells were stained with primary and secondary antibodies in pre-determined dilutions at 4°C for 15 min (see Supplementary table 3). Intracellular antigens were stained after cell surface antigens, using Sony’s Fixation and Intracellular Staining Permeabilization Wash Buffers for cytoplasmic, (Sony, 2704005, 2705010), and eBioscience’s Foxp3 / Transcription Factor Staining Buffer Set for nuclear proteins (Thermo, 00–5523-00). Cytokines and secretory molecules were analyzed after Brefeldin A treatment (eBioscience, 00–4506-51); 5 μg/ml for 2 hours at 37°C before staining. Intracellular nitric oxide (NO) levels were determined using DAF-FM staining (Thermo Fisher, D23844), as recommended by the manufacturer.

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