2 2 azobis 2 methylpropionamidine dihydrochloride v 50

Inhibited 1-Vinyl-2-pyrrolidone >99%, inhibited poly(ethylene glycol) diacrylate (575 Da), potassium persulfate (KPS) >99%, Tween 20, SPAN 80 and sodium hexametaphosphate crystalline, +200 mesh, 96% were bought from Sigma Aldrich. Potassium phosphate monobasic, ACS, crystals, cyclohexane 99%+ and 2,2^,-Azobis(2-methylpropionamidine)dihydrochloride (V-50) 98% were bought from Acros Organics. Sodium Chloride, >99.5% and acetone, HPLC grade were bought from Fisher Chemical. 2,2′-Azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (VA-044) was bought from Wako Chemicals and 2,2′-Azobis(2,4-dimethylvaleronitrile) (V-65) was bought from Fujifilm. Sodium hexametaphosphate, potassium monophosphate, Tween 20, SPAN 80, KPS, V-50, VA-044, V-65, and the solvents cyclohexane and acetone were used as received. The NVP was filtered through a 0.22 μm filter to remove the sodium hydroxide inhibitor present, while the PEGDA was passed through an inhibitor removal column to remove the hydroquinone monomethyl ether (MEHQ) inhibitor.

Acryl-PEG-SVA (MW= 5,000 g/mol) was purchased from Laysan Bio (Arab, AL). PEGDA (MW= 575 g/mol), Vinyl-2-pyrrolidone (NVP), potassium persulfate (KPS), sodium hexametaphosphate crystalline, Span® 80, Tween® 20, Eosin Y, triethanolamine (TEA), fluorescein isothiocyanate (FITC), 2,2′-(Ethylenedioxy)bis(ethylamine) (EDEA) and piperidine were purchased from Sigma Aldrich (St Louis, MO). Cyclohexane 99%, potassium phosphate monobasic crystals and 2,2’-Azobis(2-methylpropionamidine) dihydrochloride (V-50) were purchased from Acros Organics. Acetone, 1-Ethyl-3-(3-Dimethylaminopropyl)carbodiimide (EDAC), triisopropylsilane, diethyl ether and N,N-diisopropylethylamine (DIEA), were purchased from Fisher Scientific (Hanover Park, IL). All amino acids, resin, O-benzotriazole-N,N,N’,N’-tetramethyluronium-hexafluoro-phosphate (HBTU), N,N-dimethylformamide (DMF) and trifluoroacetic acid (TFA) were purchased from AAPPTec (Louisville, KY). Please add Pi and PPi names and vendor information here. Ultra-high purity (UHP) nitrogen was purchased from Airgas (Radnor, PA, USA). All the other reagents were used without further processing.