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C11440 orca flash 4

Manufactured by Hamamatsu Photonics
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The C11440 ORCA-flash 4.0 is a digital CMOS camera designed for scientific and industrial applications. It features a 4-megapixel CMOS image sensor and is capable of capturing images at high frame rates. The camera provides a straightforward interface and is suitable for a variety of imaging tasks.

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Lab products found in correlation

Alexa fluor 568, by Thermo Fisher Scientific (2 mentions) Eclipse ti2 microscope, by Nikon (2 mentions) Elements analysis 5, by Nikon (2 mentions) Micro slide 4 well plates, by Ibidi (2 mentions) Janelia fluor 646, by Novus Biologicals (2 mentions) Bx51w1, by Olympus (1 mentions) Advasep 7, by Merck Group (1 mentions) Fm1 43, by Abcam (1 mentions)

Spelling variants of this product

Orca Flash 4.0 (385 citations) Flash 4.0 (72 citations) C11440 (57 citations) Orca Flash 4.0 C11440 (13 citations) ORCA-Flash 4.0 LT C11440 camera (3 citations) Orca®-flash 4.0 V2 Digital CMOS camera C11440-22CU (3 citations) ORCA Flash 4.0 V2 C11440-22C camera (3 citations) C11440 Orca-Flash 4.0LT (2 citations) C11440-22C ORCA-Flash 4.0 v2 (2 citations) Orca Flash 4.0 C11440-22C sCMOS camera (1 citations)

3 protocols using c11440 orca flash 4

1

Super-Resolution Imaging of INTS1 and RPB1

Cited 1 time
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Cells (4x103) were plated onto micro slide 4 well plates (Ibidi), fixed in 4% formaldehyde, and immunostained with appropriate primary and secondary antibodies. Imaging experiments were carried out with a Nikon eclipse Ti2 microscope equipped with Nikon Instruments (NSTORM). For two color imaging dSTORM imaging, Janelia Fluor 646 [Novus Bio NBP1-7-2739JF646 Lot #36481-051615] was used as a secondary staining for INTS1 and Alexa Fluor 568 [Invitrogen A11077 Lot #1917936] was used as a secondary staining for RPB1 Tyr-1. The secondary antibodies were used with MEA STORM imaging buffer and were imaged continuously with 5000 frames collected per filter range at a frequency of 30 ms. The images were acquired using a 100x, 1.49 NA objective, and imaged onto a Hamamatsu C11440 ORCA-flash 4.0. Storm analysis was carried out with Nikon Elements Analysis 5.02.01 for identification of molecules. Molecule list files were then exported from Nikon elements to be further analyzed using software Clus-Doc software in MATLAB R2018b (Pageon et al., 2016 (link)). Cluster density analysis, specifically DBSCAN function, was carried out after manually selecting region of interest corresponding to the nucleus. Statistical significance and graphing were performed using Prism software.
Beckedorff F., Blumenthal E., daSilva L.F., Aoi Y., Cingaram P.R., Yue J., Zhang A., Dokaneheifard S., Valencia M.G., Gaidosh G., Shilatifard A, & Shiekhattar R. (2020). The Human Integrator Complex Facilitates Transcriptional Elongation by Endonucleolytic Cleavage of Nascent Transcripts. Cell reports, 32(3), 107917.
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2

Super-Resolution Imaging of INTS1 and RPB1

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells (4x103) were plated onto micro slide 4 well plates (Ibidi), fixed in 4% formaldehyde, and immunostained with appropriate primary and secondary antibodies. Imaging experiments were carried out with a Nikon eclipse Ti2 microscope equipped with Nikon Instruments (NSTORM). For two color imaging dSTORM imaging, Janelia Fluor 646 [Novus Bio NBP1-7-2739JF646 Lot #36481-051615] was used as a secondary staining for INTS1 and Alexa Fluor 568 [Invitrogen A11077 Lot #1917936] was used as a secondary staining for RPB1 Tyr-1. The secondary antibodies were used with MEA STORM imaging buffer and were imaged continuously with 5000 frames collected per filter range at a frequency of 30 ms. The images were acquired using a 100x, 1.49 NA objective, and imaged onto a Hamamatsu C11440 ORCA-flash 4.0. Storm analysis was carried out with Nikon Elements Analysis 5.02.01 for identification of molecules. Molecule list files were then exported from Nikon elements to be further analyzed using software Clus-Doc software in MATLAB R2018b (Pageon et al., 2016 (link)). Cluster density analysis, specifically DBSCAN function, was carried out after manually selecting region of interest corresponding to the nucleus. Statistical significance and graphing were performed using Prism software.
Beckedorff F., Blumenthal E., daSilva L.F., Aoi Y., Cingaram P.R., Yue J., Zhang A., Dokaneheifard S., Valencia M.G., Gaidosh G., Shilatifard A, & Shiekhattar R. (2020). The Human Integrator Complex Facilitates Transcriptional Elongation by Endonucleolytic Cleavage of Nascent Transcripts. Cell reports, 32(3), 107917.
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3

Imaging Presynaptic Vesicle Release in Spinal Cord

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Lumbar spinal cord slices were incubated in Mg2+-free ACSF that contained (mM): 125 NaCl, 2.5 KCl, 1.25 NaH2PO4, 25 NaHCO3, 2.5 CaCl2, 10 glucose and 0.1 4-AP for 10 min to increase neuronal activity. Slices were transferred to Mg2+-free ACSF with 4-AP containing 8 μM FM1–43 (Abcam, Australia) for 3 min, washed with ACSF for 2 min, and then incubated in 1 mM ADVASEP-7 (Sigma) for 2 min. Slices were washed with ACSF for 2 min and the ADVASEP-7 incubation was repeated. Slices were placed in the recording chamber and washed for a further 15 min in ACSF prior to imaging. A bipolar stimulating electrode was placed in the dorsal root entry zone of the spinal cord and stimulated for 10 s at 1Hz, 4–6V to facilitate the release of vesicles from the presynaptic terminals. Optical recordings were completed on an upright fluorescence microscope (BX51W1, Olympus) under 40x magnification using a Cy3/TRITC filter set CCD camera (C11440 Orca Flash 4.0, Hamamatsu). Image sequences were analyzed using Fiji NIH image software.
Tonello R., Anderson W.B., Davidson S., Escriou V., Yang L., Schmidt B.L., Imlach W.L, & Bunnett N.W. (2022). The contribution of endocytosis to sensitization of nociceptors and synaptic transmission in nociceptive circuits. Pain, 164(6), 1355-1374.
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