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Elisa kits for measuring irisin

Manufactured by Abcam
Sourced in Canada

ELISA kits for measuring Irisin are laboratory tools used to quantify the concentration of the protein Irisin in biological samples. These kits utilize the Enzyme-Linked Immunosorbent Assay (ELISA) technique to detect and measure the level of Irisin present in the sample.

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2 protocols using elisa kits for measuring irisin

1

Molecular Markers of Metabolic Health

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The primary antibodies against p-Akt (Ser473), Akt, p-Smad2, p-Smad3, PPARγ, p-ACC1 and PGC1-α were from Cell Signaling Technology (Beverly, MA). Antibodies against F4/80, CD68, Fndc5 and UCP-1 were from Abcam (Cambridge, MA). ELISA kits for measuring irisin, IL-6 or adiponectin were from BioVision (Milpitas, CA), eBioscience (San Diego, CA) or R&D Systems (Minneapolis, MN) respectively. The recombinant proteins, irisin or myostatin were obtained from Enzo life Sciences (Farmingdale, NY) or R&D systems. The anti-myostatin peptibody (peptibody) was from Atara Biotherapeutics (Westlake Village, CA) (21 (link);22 (link)). Serum insulin concentration was measured using the Rat/Mouse Insulin ELISA kit (Millipore, Billerica, MA). Serum free fatty acid levels were measured using the NEFA C kit from Wako (Richmond, VA).
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2

Molecular Markers of Metabolic Health

Check if the same lab product or an alternative is used in the 5 most similar protocols
The primary antibodies against p-Akt (Ser473), Akt, p-Smad2, p-Smad3, PPARγ, p-ACC1 and PGC1-α were from Cell Signaling Technology (Beverly, MA). Antibodies against F4/80, CD68, Fndc5 and UCP-1 were from Abcam (Cambridge, MA). ELISA kits for measuring irisin, IL-6 or adiponectin were from BioVision (Milpitas, CA), eBioscience (San Diego, CA) or R&D Systems (Minneapolis, MN) respectively. The recombinant proteins, irisin or myostatin were obtained from Enzo life Sciences (Farmingdale, NY) or R&D systems. The anti-myostatin peptibody (peptibody) was from Atara Biotherapeutics (Westlake Village, CA) (21 (link);22 (link)). Serum insulin concentration was measured using the Rat/Mouse Insulin ELISA kit (Millipore, Billerica, MA). Serum free fatty acid levels were measured using the NEFA C kit from Wako (Richmond, VA).
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