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Spike 2

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Spike 2 is a lab equipment product developed by ACROBiosystems. It is a tool used for the analysis and characterization of protein structures, specifically the spike protein of various viruses. The core function of Spike 2 is to provide researchers with a reliable and efficient means to study the structural properties of spike proteins, which are crucial components of viral entry mechanisms.

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Lab products found in correlation

Magnetic carboxylated beads, by Bio-Rad (2 mentions) Tetanus toxoid, by Merck Group (2 mentions) Sars cov 2 spike 1, by Sino Biological (2 mentions) Sars cov 2 and hku 1 spike trimers, by BPS Biosciences (2 mentions) Sars cov and nl63 spike trimers, by BPS Biosciences (2 mentions) Influenza hemagglutinin h1cal2009, by Sino Biological (2 mentions) Siv gp120, by Sino Biological (2 mentions) Nucleoprotein, by ACROBiosystems (1 mentions)

Spelling variants of this product

Anti-SARS-CoV-2 spike broadly neutralizing antibody (human IgG4; clone no. AM359b) (2 citations) Recombinant SARS-CoV-2 Spike protein (2 citations) SARS-CoV-2 Spike RBD human monoclonal antibody (1 citations)

2 protocols using spike 2

1

Multiplex SARS-CoV-2 Antibody Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
As previously described25 , a custom multiplex bead array was designed and coupled with SARS-CoV-2 spike 1 (Sino Biological), spike 2 (ACRO Biosystems), RBD (BEI Resources) and nucleoprotein (ACRO Biosystems), as well as SARS and hCoV (229E, NL63, HKU1, OC43) spikes and nucleoproteins (Sino Biological) (Supplementary Fig. 5). In addition, SARS-CoV-2 and HKU-1 spike trimers (kind gifts from Adam K. Wheatley), as well as SARS-CoV and NL63 spike trimers (BPS Bioscience) were also coupled. Tetanus toxoid (Sigma-Aldrich), influenza hemagglutinin (H1Cal2009; Sino Biological) and SIV gp120 (Sino Biological) were also included in the assay as positive and negative controls respectively. Antigens were covalently coupled to magnetic carboxylated beads (Bio Rad) using a two-step carbodiimide reaction and blocked with 0.1% BSA, before being resuspended and stored in PBS 0.05% sodium azide.
Zhang W., Chua B., Selva K., Kedzierski L., Ashhurst T., Haycroft E., Shoffner S., Hensen L., Boyd D., James F., Mouhtouris E., Kwong J., Chua K., Drewett G., Copaescu A., Dobson J., Rowntree L., Habel J., Allen L., Koay H.F., Neil J., Gartner M., Lee C., Andersson P., Seemann T., Sherry N., Amanat F., Krammer F., Londrigan S., Wakim L., King N., Godfrey D., Mackay L., Thomas P., Nicholson S., Arnold K., Chung A., Holmes N., Smibert O., Trubiano J., Gordon C., Nguyen T, & Kedzierska K. (2021). Immune responses in COVID-19 respiratory tract and blood reveal mechanisms of disease severity. Research Square.
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2

SARS-CoV-2 Multiplex Serological Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
As previously described25 (link), a custom multiplex bead array was designed and coupled with SARS-CoV-2 spike 1 (Sino Biological), spike 2 (ACRO Biosystems), RBD (BEI Resources) and nucleoprotein (ACRO Biosystems), as well as SARS and hCoV (229E, NL63, HKU1, OC43) spikes and nucleoproteins (Sino Biological) (Supplementary Table 5). In addition, SARS-CoV-2 and HKU-1 spike trimers (kind gifts from Adam K. Wheatley), as well as SARS-CoV and NL63 spike trimers (BPS Bioscience) were also coupled. Tetanus toxoid (Sigma-Aldrich), influenza hemagglutinin (H1Cal2009; Sino Biological) and SIV gp120 (Sino Biological) were included in the assay as positive and negative controls respectively. Antigens were covalently coupled to magnetic carboxylated beads (Bio Rad) using a two-step carbodiimide reaction and blocked with 0.1% BSA, before being resuspended and stored in PBS 0.05% sodium azide.
Zhang W., Chua B.Y., Selva K.J., Kedzierski L., Ashhurst T.M., Haycroft E.R., Shoffner-Beck S.K., Hensen L., Boyd D.F., James F., Mouhtouris E., Kwong J.C., Chua K.Y., Drewett G., Copaescu A., Dobson J.E., Rowntree L.C., Habel J.R., Allen L.F., Koay H.F., Neil J.A., Gartner M.J., Lee C.Y., Andersson P., Khan S.F., Blakeway L., Wisniewski J., McMahon J.H., Vine E.E., Cunningham A.L., Audsley J., Thevarajan I., Seemann T., Sherry N.L., Amanat F., Krammer F., Londrigan S.L., Wakim L.M., King N.J., Godfrey D.I., Mackay L.K., Thomas P.G., Nicholson S., Arnold K.B., Chung A.W., Holmes N.E., Smibert O.C., Trubiano J.A., Gordon C.L., Nguyen T.H, & Kedzierska K. (2022). SARS-CoV-2 infection results in immune responses in the respiratory tract and peripheral blood that suggest mechanisms of disease severity. Nature Communications, 13, 2774.
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